1887

Abstract

A novel actinomycete, strain FMN03, was isolated from a soil sample collected from Yuga Zapadnaya South-West Forest Park, Moscow, Russia. The isolate had chemical and morphological properties typical of members of the genus and formed a distinct 16S rRNA gene subclade with the type strains subsp. NBRC 15903 and subsp. NBRC 14098. The organism formed extensively branched substrate and aerial hyphae, which generated spiral chains of spores with smooth surfaces. The cell wall contained -diaminopimelic acid and the whole cell sugars were glucose, galactose and trace amounts of madurose, mannose and xylose. The polar lipids were phosphatidylethanolamine, hydroxyphosphatidylethanolamine, four unidentified phospholipids, four unidentified glycolipids and one unidentified lipid. The predominant menaquinone was MK-9(H). The major fatty acids were iso-C 2-OH, C 10-methyl, C 9 and iso-C. Analyses of its morphological, physiological and biochemical characteristics, together with DNA–DNA relatedness data, confirmed that strain FMN03 is a representative of a novel species of the genus Nonomuraea, which is distinct from closely related reference strains. Strain FMN03 ( = DSM 45913 = KCTC 29233) is proposed as the type strain of a novel species, for which the name sp. nov. is proposed.

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2014-07-01
2019-10-19
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