1887

Abstract

Two bacterial strains, HKU33 and HKU34, were isolated in Hong Kong from the pus aspirated from the right peritonsillar abscess of a patient with quinsy and the left elbow joint fluid of another patient with tophaceous gout and left elbow septic arthritis, respectively. The bacteria were Gram-stain-negative, non-motile, non-spore-forming, non-haemolytic pleomorphic bacilli. They grew best on Columbia agar with 5 % defibrinated sheep blood in an anaerobic environment or aerobic environment with 5 % CO. They also grew on chocolate agar but not on MacConkey agar. They were catalase- and cytochrome oxidase-negative. They showed a unique profile of enzyme activities distinguishable from their closely related species. Phylogenetic analysis of the complete 16S rRNA gene, and partial , and gene sequences showed the two isolates formed a distinct branch within the family , being related most closely to . Hierarchical cluster analysis of mass spectra of whole-cell protein contents showed that strains HKU33 and HKU34 were closely related to each other, but were distinct from , and ‘’. The DNA G+C content of strain HKU33 was 26.0±2.1 mol% (mean±;  = 3). DNA–DNA hybridization demonstrated ≤45.02 % DNA relatedness between the two isolates and CCUG 13453. A novel species, sp. nov., is proposed to accommodate strains HKU33 and HKU34, with HKU33 ( = JCM 18691 = NCTC 13659 = DSM 26322) designated the type strain. Emended descriptions of the genus and are also given.

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2014-09-01
2019-11-12
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