1887

Abstract

A Gram-stain-negative, elongated rod-shaped, motile by gliding, green-pigmented, aerobic bacterial strain, designated LY03, was isolated from lake water in Xiamen, Fujian Province, China. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the isolate was a member of the genus , which belongs to the family . Strain LY03 was most closely related to LMG 22011 (96.02 % 16S rRNA gene sequence similarity), followed by KACC 11467 (94.85 %), and the three strains formed a distinct lineage from other strains in the phylogenetic analyses. Optimum conditions for growth were 37 °C, pH 7–9 and without NaCl. The major fatty acids were summed feature 3 (Cω6 and/or Cω7), C and C 3-OH. The DNA G+C content of strain LY03 was 63.6 mol% and the major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unknown polar lipids and unidentified phospholipids. Differential phenotypic properties and phylogenetic distinctiveness distinguished strain LY03 from all other members of the genus . On the basis of its morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, strain LY03 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LY03 ( = MCCC 1F01209 = KCTC 32574).

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2014-09-01
2019-11-17
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References

  1. Ausubel F. M. , Brent R. , Kingston R. E. , Moore D. D. , Seidman J. G. , Smith J. A. , Struhl K. . (editors) ( 1995; ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, , 3rd edn.. New York:: Wiley;.
    [Google Scholar]
  2. Bernardet J.-F. , Nakagawa Y. , Holmes B. . Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bowman J. P. . ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  4. Chang S.-C. , Wang J.-T. , Vandamme P. , Hwang J.-H. , Chang P.-S. , Chen W.-M. . ( 2004; ). Chitinimonas taiwanensis gen. nov., sp. nov., a novel chitinolytic bacterium isolated from a freshwater pond for shrimp culture. . Syst Appl Microbiol 27:, 43–49. [CrossRef] [PubMed]
    [Google Scholar]
  5. DeLong E. F. . ( 1992; ). Archaea in coastal marine environments. . Proc Natl Acad Sci U S A 89:, 5685–5689. [CrossRef] [PubMed]
    [Google Scholar]
  6. Kates M. . ( 1986; ). Techniques of Lipidology isolation, analysis and identification of lipids, , 2nd ed. rev.., pp. 106–107, 241–246. Amsterdam:: Elsevier;.
    [Google Scholar]
  7. Kim B.-Y. , Weon H.-Y. , Yoo S.-H. , Chen W.-M. , Kwon S.-W. , Go S.-J. , Stackebrandt E. . ( 2006; ). Chitinimonas koreensis sp. nov., isolated from greenhouse soil in Korea. . Int J Syst Evol Microbiol 56:, 1761–1764. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  9. Li Y. , Bai S. , Yang C. , Lai Q. , Zhang H. , Chen Z. , Wei J. , Zheng W. , Tian Y. , Zheng T. . ( 2013a; ). Mangrovimonas yunxiaonensis gen. nov., sp. nov., isolated from mangrove sediment. . Int J Syst Evol Microbiol 63:, 2043–2048. [CrossRef] [PubMed]
    [Google Scholar]
  10. Li Y. , Wei J. , Yang C. , Lai Q. , Chen Z. , Li D. , Zhang H. , Tian Y. , Zheng W. , Zheng T. . ( 2013b; ). Tenacibaculum xiamenense sp. nov., an algicidal bacterium isolated from coastal seawater. . Int J Syst Evol Microbiol 63:, 3481–3486. [CrossRef] [PubMed]
    [Google Scholar]
  11. Rzhetsky A. , Nei M. . ( 1993; ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. . Mol Biol Evol 10:, 1073–1095.[PubMed]
    [Google Scholar]
  12. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  13. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  14. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS microbiology letters 25:, 125–128.[CrossRef]
    [Google Scholar]
  15. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  16. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
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