%0 Journal Article %A Suarez, Christian %A Ratering, Stefan %A Geissler-Plaum, Rita %A Schnell, Sylvia %T Rheinheimera hassiensis sp. nov. and Rheinheimera muenzenbergensis sp. nov., two species from the rhizosphere of Hordeum secalinum %D 2014 %J International Journal of Systematic and Evolutionary Microbiology, %V 64 %N Pt_4 %P 1202-1209 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.061200-0 %I Microbiology Society, %X Two motile, Gram-staining-negative, aerobic, rod-shaped bacteria designated strains E48T and E49T were isolated from the rhizosphere of Hordeum secalinum from a natural salt meadow near Münzenberg, Germany. 16S rRNA gene sequence similarity analysis revealed that strains E48T and E49T shared similarities of 97.6 % with Rheinheimera pacifica KMM 1406T and 98.5 % with Rheinheimera nanhaiensis E407-8T, respectively. Major fatty acids of strain E48T were C16 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C17 : 1ω8c, and of strain E49T were C16 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C18 : 1ω7c. The DNA G+C contents were 50.5 mol% (E48T) and 50.0 mol% (E49T). Strains E48T and E49T grew at 4–37 °C (optimum 28 °C) and with 0–6 % NaCl (optimum 0–3 %) and 0–5 % NaCl (optimum 0–3 %), respectively. The potential for nitrogen fixation by strains E48T and E49T was evaluated by molecular techniques and the acetylene reduction assay. The DNA–DNA hybridization, physiological and molecular data demonstrated that strains E48T and E49T represent two novel species of the genus Rheinheimera , and therefore the names Rheinheimera hassiensis sp. nov. (type strain E48T = LMG 27268T = KACC 17070T) and Rheinheimera muenzenbergensis sp. nov. (type strain E49T = LMG 27269T = KACC 17071T) are proposed. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.061200-0