1887

Abstract

A Gram-stain-negative, motile, mesophilic, aerobic, rod-shaped bacterium, strain 8-8, was isolated from surface seawater at Muroto, Kochi, Japan. The strain exhibited agar-degrading activity. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the family and clustered distantly with members of the genus (≤94.0 % similarity). The DNA G+C content was 41.8 mol%. The major fatty acids were Cω7 and/or iso-C 2-OH, C and Cω7 and the major hydroxy fatty acid was C 3-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unidentified polar lipid; lysophosphatidylethanolamine and unidentified polar lipids were found as minor components. The major quinone was Q-8. On the basis of phenotypic, genotypic and chemotaxonomic data, strain 8-8 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is 8-8 ( = NBRC 110023 = LMG 28167).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.061150-0
2014-07-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/7/2416.html?itemId=/content/journal/ijsem/10.1099/ijs.0.061150-0&mimeType=html&fmt=ahah

References

  1. Akagawa-Matsushita M., Itoh T., Katayama Y., Kuraishi H., Yamasato K.. ( 1992;). Isoprenoid quinone composition of some marine Alteromonas, Marinomonas, Deleya, Pseudomonas and Shewanella species. . Microbiology 138:, 2275–2281.
    [Google Scholar]
  2. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J.. ( 1990;). Basic local alignment search tool. . J Mol Biol 215:, 403–410. [CrossRef][PubMed]
    [Google Scholar]
  3. Baik K. S., Park Y. D., Seong C. N., Kim E. M., Bae K. S., Chun J.. ( 2006;). Glaciecola nitratireducens sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 56:, 2185–2188. [CrossRef][PubMed]
    [Google Scholar]
  4. Barbeyron T., L’Haridon S., Corre E., Kloareg B., Potin P.. ( 2001;). Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov.. Int J Syst Evol Microbiol 51:, 985–997. [CrossRef][PubMed]
    [Google Scholar]
  5. Barrow G. I., Feltham R. K. A.. (editors) ( 1993;). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  6. Baumann L., Baumann P., Mandel M., Allen R. D.. ( 1972;). Taxonomy of aerobic marine eubacteria. . J Bacteriol 110:, 402–429.[PubMed]
    [Google Scholar]
  7. Baumann P., Baumann L., Bowditch R. D., Beaman B.. ( 1984;). Taxonomy of Alteromonas: A. nigrifaciens sp. nov., nom. rev.; A. macleodii; and A. haloplanktis. . Int J Syst Bacteriol 34:, 145–149. [CrossRef]
    [Google Scholar]
  8. Bowman J. P., McCammon S. A., Brown J. L., McMeekin T. A.. ( 1998;). Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats. . Int J Syst Bacteriol 48:, 1213–1222. [CrossRef]
    [Google Scholar]
  9. Chen L. P., Xu H. Y., Fu S. Z., Fan H. X., Liu Y. H., Liu S. J., Liu Z. P.. ( 2009;). Glaciecola lipolytica sp. nov., isolated from seawater near Tianjin city, China. . Int J Syst Evol Microbiol 59:, 73–76. [CrossRef][PubMed]
    [Google Scholar]
  10. Conklin E. J., Smith J. E.. ( 2005;). Abundance and spread of the invasive red algae, Kappaphycus spp., in Kane’ohe Bay, Hawai’i and an experimental assessment of management options. . Biol Invasions 7:, 1029–1039. [CrossRef]
    [Google Scholar]
  11. DeLong E. F., Preston C. M., Mincer T., Rich V., Hallam S. J., Frigaard N. U., Martinez A., Sullivan M. B., Edwards R.. & other authors ( 2006;). Community genomics among stratified microbial assemblages in the ocean’s interior. . Science 311:, 496–503. [CrossRef][PubMed]
    [Google Scholar]
  12. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  13. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  14. Gauthier G., Gauthier M., Christen R.. ( 1995;). Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. . Int J Syst Bacteriol 45:, 755–761. [CrossRef][PubMed]
    [Google Scholar]
  15. Hansman R. L., Griffin S., Watson J. T., Druffel E. R., Ingalls A. E., Pearson A., Aluwihare L. I.. ( 2009;). The radiocarbon signature of microorganisms in the mesopelagic ocean. . Proc Natl Acad Sci U S A 106:, 6513–6518. [CrossRef][PubMed]
    [Google Scholar]
  16. Ivanova E. P., Zhukova N. V., Svetashev V. I., Gorshkova N. M., Kurilenko V. V., Frolova G. M., Mikhailov V. V.. ( 2000;). Evaluation of phospholipid and fatty acid compositions as chemotaxonomic markers of Alteromonas-like proteobacteria. . Curr Microbiol 41:, 341–345. [CrossRef][PubMed]
    [Google Scholar]
  17. Jean W. D., Hsu C. Y., Huang S.-P., Chen J.-S., Lin S., Su M.-H., Shieh W. Y.. ( 2013;). Reclassification of [Glaciecola] lipolytica and [Aestuariibacter] litoralis in Aliiglaciecola gen. nov., as Aliiglaciecola lipolytica comb. nov. and Aliiglaciecola litoralis comb. nov., respectively. . Int J Syst Evol Microbiol 63:, 2859–2864. [CrossRef][PubMed]
    [Google Scholar]
  18. Katayama-Fujimura Y., Komatsu Y., Kuraishi H., Kaneko T.. ( 1984;). Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. . Agric Biol Chem 48:, 3169–3172. [CrossRef]
    [Google Scholar]
  19. Kurahashi M., Yokota A.. ( 2004;). Agarivorans albus gen. nov., sp. nov., a γ-proteobacterium isolated from marine animals. . Int J Syst Evol Microbiol 54:, 693–697. [CrossRef][PubMed]
    [Google Scholar]
  20. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A.. & other authors ( 2007;). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef][PubMed]
    [Google Scholar]
  21. Leifson E.. ( 1963;). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  22. Marmur J.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  23. Matsuyama H., Hirabayashi T., Kasahara H., Minami H., Hoshino T., Yumoto I.. ( 2006;). Glaciecola chathamensis sp. nov., a novel marine polysaccharide-producing bacterium. . Int J Syst Evol Microbiol 56:, 2883–2886. [CrossRef][PubMed]
    [Google Scholar]
  24. Nakagawa Y., Yamasato K.. ( 1996;). Emendation of the genus Cytophaga and transfer of Cytophaga agarovorans and Cytophaga salmonicolor to Marinilabilia gen. nov.: phylogenetic analysis of the Flavobacterium-Cytophaga complex. . Int J Syst Bacteriol 46:, 599–603. [CrossRef]
    [Google Scholar]
  25. Nedashkovskaya O. I., Kim S. B., Vancanneyt M., Shin D. S., Lysenko A. M., Shevchenko L. S., Krasokhin V. B., Mikhailov V. V., Swings J., Bae K. S.. ( 2006;). Salegentibacter agarivorans sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the sponge Artemisina sp.. Int J Syst Evol Microbiol 56:, 883–887. [CrossRef][PubMed]
    [Google Scholar]
  26. Nishijima M., Araki-Sakai M., Sano H.. ( 1997;). Identification of isoprenoid quinones by frit-FAB liquid chromatography–mass spectrometry for the chemotaxonomy of microorganisms. . J Microbiol Methods 28:, 113–122. [CrossRef]
    [Google Scholar]
  27. Park S., Yoon J. H.. ( 2013;). Glaciecola aquimarina sp. nov., a gammaproteobacterium isolated from coastal seawater. . Antonie van Leeuwenhoek 103:, 1141–1148. [CrossRef][PubMed]
    [Google Scholar]
  28. Romanenko L. A., Zhukova N. V., Rohde M., Lysenko A. M., Mikhailov V. V., Stackebrandt E.. ( 2003a;). Glaciecola mesophila sp. nov., a novel marine agar-digesting bacterium. . Int J Syst Evol Microbiol 53:, 647–651. [CrossRef][PubMed]
    [Google Scholar]
  29. Romanenko L. A., Zhukova N. V., Rohde M., Lysenko A. M., Mikhailov V. V., Stackebrandt E.. ( 2003b;). Pseudoalteromonas agarivorans sp. nov., a novel marine agarolytic bacterium. . Int J Syst Evol Microbiol 53:, 125–131. [CrossRef][PubMed]
    [Google Scholar]
  30. Saito H., Miura K. I.. ( 1963;). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72:, 619–629. [CrossRef][PubMed]
    [Google Scholar]
  31. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  32. Svetashev V. I., Yysotskii M. V., Ivanova E. P., Mikhailov V. V.. ( 1995;). Cellular fatty acids of Alteromonas species. . Syst Appl Microbiol 18:, 37–43. [CrossRef]
    [Google Scholar]
  33. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  34. Tanaka N., Romanenko L. A., Frolova G. M., Mikhailov V. V.. ( 2010;). Aestuariibacter litoralis sp. nov., isolated from a sandy sediment of the Sea of Japan. . Int J Syst Evol Microbiol 60:, 317–320. [CrossRef][PubMed]
    [Google Scholar]
  35. Teramoto M., Suzuki M., Okazaki F., Hatmanti A., Harayama S.. ( 2009;). Oceanobacter-related bacteria are important for the degradation of petroleum aliphatic hydrocarbons in the tropical marine environment. . Microbiology 155:, 3362–3370. [CrossRef][PubMed]
    [Google Scholar]
  36. Teramoto M., Ohuchi M., Hatmanti A., Darmayati Y., Widyastuti Y., Harayama S., Fukunaga Y.. ( 2011;). Oleibacter marinus gen. nov., sp. nov., a bacterium that degrades petroleum aliphatic hydrocarbons in a tropical marine environment. . Int J Syst Evol Microbiol 61:, 375–380. [CrossRef][PubMed]
    [Google Scholar]
  37. Van Trappen S., Tan T. L., Yang J., Mergaert J., Swings J.. ( 2004;). Glaciecola polaris sp. nov., a novel budding and prosthecate bacterium from the Arctic Ocean, and emended description of the genus Glaciecola. . Int J Syst Evol Microbiol 54:, 1765–1771. [CrossRef][PubMed]
    [Google Scholar]
  38. Yi H., Bae K. S., Chun J.. ( 2004;). Aestuariibacter salexigens gen. nov., sp. nov. and Aestuariibacter halophilus sp. nov., isolated from tidal flat sediment, and emended description of Alteromonas macleodii. . Int J Syst Evol Microbiol 54:, 571–576. [CrossRef][PubMed]
    [Google Scholar]
  39. Yong J. J., Park S. J., Kim H. J., Rhee S. K.. ( 2007;). Glaciecola agarilytica sp. nov., an agar-digesting marine bacterium from the East Sea, Korea. . Int J Syst Evol Microbiol 57:, 951–953. [CrossRef][PubMed]
    [Google Scholar]
  40. Yoon J. H., Kang S. J., Lee M. H., Oh T. K.. ( 2008;). Tamlana agarivorans sp. nov., isolated from seawater off Jeju Island in Korea. . Int J Syst Evol Microbiol 58:, 1892–1895. [CrossRef][PubMed]
    [Google Scholar]
  41. Zhang D. C., Yu Y., Chen B., Wang H. X., Liu H. C., Dong X. Z., Zhou P. J.. ( 2006;). Glaciecola psychrophila sp. nov., a novel psychrophilic bacterium isolated from the Arctic. . Int J Syst Evol Microbiol 56:, 2867–2869. [CrossRef][PubMed]
    [Google Scholar]
  42. Zhang Y. J., Zhang X. Y., Mi Z. H., Chen C. X., Gao Z. M., Chen X. L., Yu Y., Chen B., Zhang Y. Z.. ( 2011;). Glaciecola arctica sp. nov., isolated from Arctic marine sediment. . Int J Syst Evol Microbiol 61:,2338–2341. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.061150-0
Loading
/content/journal/ijsem/10.1099/ijs.0.061150-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error