1887

Abstract

A Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, aerobic bacterial strain, designated BUT-2, was isolated from activated sludge of one herbicide-manufacturing wastewater-treatment facility in Kunshan, Jiangsu province, China, and subjected to polyphasic taxonomic studies. Analysis of the 16S rRNA gene sequence indicated that strain BUT-2 shared the highest similarity with (98.98 %), followed by (98.88 %), with less than 96 % similarlity to members of the genera , , and . Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain BUT-2 clustered with JC16 and MW10, occupying a distinct phylogenetic position. The major fatty acid (>10 % of total fatty acids) type of strain BUT-2 was iso-C The quinone system comprised menaquinone MK-7 (77.8 %), MK-6 (11.9 %) and MK-8 (10.3 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and some unidentified phospholipids. The cell-wall peptidoglycan type of strain BUT-2 was -Orn--Glu. The genomic DNA G+C content of strain BUT-2 was 48.5 mol%. Furthermore, the DNA–DNA relatedness in hybridization experiments against the reference strain was lower than 70 %, confirming that strain BUT-2 did not belong to previously described species of the genus . On the basis of its morphological, physiological and chemotaxonomic characteristics as well as phylogenetic analysis, strain BUT-2 is considered to represent a novel species of the genus , for which the name Chryseomicrobium sp. nov. is proposed. The type strain is BUT-2 ( = CCTCC AB2013082 = KACC 17219).

Funding
This study was supported by the:
  • , National Science and Technology Support Plan , (Award 2012BAD15B03 and 2013AA102804)
  • , National Natural Science Foundation of China , (Award 31200087 and 31270157)
  • , Fundamental Research Funds for the Central Universities , (Award KYZ201122)
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2014-08-01
2020-10-24
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