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Volume 64, Issue Pt_8

sp. nov., a bacterium isolated from activated sludge Free

Abstract

A Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, aerobic bacterial strain, designated BUT-2, was isolated from activated sludge of one herbicide-manufacturing wastewater-treatment facility in Kunshan, Jiangsu province, China, and subjected to polyphasic taxonomic studies. Analysis of the 16S rRNA gene sequence indicated that strain BUT-2 shared the highest similarity with (98.98 %), followed by (98.88 %), with less than 96 % similarlity to members of the genera , , and . Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain BUT-2 clustered with JC16 and MW10, occupying a distinct phylogenetic position. The major fatty acid (>10 % of total fatty acids) type of strain BUT-2 was iso-C The quinone system comprised menaquinone MK-7 (77.8 %), MK-6 (11.9 %) and MK-8 (10.3 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and some unidentified phospholipids. The cell-wall peptidoglycan type of strain BUT-2 was -Orn--Glu. The genomic DNA G+C content of strain BUT-2 was 48.5 mol%. Furthermore, the DNA–DNA relatedness in hybridization experiments against the reference strain was lower than 70 %, confirming that strain BUT-2 did not belong to previously described species of the genus . On the basis of its morphological, physiological and chemotaxonomic characteristics as well as phylogenetic analysis, strain BUT-2 is considered to represent a novel species of the genus , for which the name Chryseomicrobium sp. nov. is proposed. The type strain is BUT-2 ( = CCTCC AB2013082 = KACC 17219).

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Funding
This study was supported by the:
  • National Science and Technology Support Plan (Award 2012BAD15B03 and 2013AA102804)
  • National Natural Science Foundation of China (Award 31200087 and 31270157)
  • Fundamental Research Funds for the Central Universities (Award KYZ201122)
© 2014 IUMS
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2014-08-01
2024-04-19
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Chryseomicrobiumaureum sp. nov., a bacterium isolated from activated sludge
Int J Syst Evol Microbiol 64, 2682 (2014); https://doi.org/10.1099/ijs.0.061143-0
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