@article{mbs:/content/journal/ijsem/10.1099/ijs.0.061119-0, author = "Wang, Huimin and Xiang, Tingsheng and Wang, Yanwei and Song, Jinlong and Zhai, Yi and Chen, Xiaorong and Li, Yanting and Zhao, Bingqiang and Zhao, Bin and Ruan, Zhiyong", title = "Microbacterium petrolearium sp. nov., isolated from an oil-contaminated water sample", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2014", volume = "64", number = "Pt_12", pages = "4168-4172", doi = "https://doi.org/10.1099/ijs.0.061119-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.061119-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-staining-positive, rod-shaped, aerobic bacterium, designated strain LAM0410T, was isolated from an oil-contaminated water sample from the Dagang Oilfield, PR China. The temperature and pH ranges for growth were 4 °C to 50 °C and pH 4.4 to pH 12.0, respectively. The strain did not need NaCl for growth, but could tolerate a concentration of up to 12 % (w/v). Cell wall hydrolysates from the isolate showed that the diamino acid was ornithine. The cell wall sugars contained ribose and galactose. The glycan moiety of the cell wall contained N-glycolyl residues. The major respiratory quinones were MK-10, MK-11 and MK-12. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and nine unknown glycolipids. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Microbacterium and was closely related to Microbacterium sediminis MCCC 1A06153T and Microbacterium murale DSM 22178T with 97.5 % and 97.4 % sequence similarities, respectively; while the DNA–DNA hybridization values were 33.1±3.4 % and 21.8±1.6 %, respectively. The DNA G+C content was 67.4 mol%, as determined by the T m method. Based on its phenotypic and genotypic properties, strain LAM0410T represents a novel species of the genus Microbacterium , for which the name Microbacterium petrolearium sp. nov. is proposed; the type strain is LAM0410T ( = ACCC 00719T = JCM 19612T).", }