sp. nov. isolated from a chicken Free

Abstract

A Gram-stain-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (strain 280) isolated from a chicken was studied for its taxonomic allocation. 16S rRNA gene sequence analyses clearly allocated the isolate in the genus group with a 16S rRNA gene sequence similarity of 98.8 % to the currently recognized species, and . This allocation was confirmed by the fatty acid data (major fatty acids: Cω7 and C cyclo ω8) and a polyamine pattern with the major compound putrescine and relatively high amounts of spermidine. Also, the polar lipid profile with phosphatidylethanolamine, phosphatiylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine and the genus-specific ‘stretched aminolipid’ was well in line with the description of the genus . The quinone system consisted predominantly of ubiquinone Q-10 with traces of Q-9 and Q-11. DNA–DNA hybridization of strain 280 with Sa25 and KMM 3858 showed relatedness values of 38.8 % (reciprocal 20.2 %) and 30.2 % (reciprocal 29.8 %), respectively. These results in combination with differentiating physiological and biochemical data clearly showed that strain 280 merits species status. We propose the name sp. nov. to accommodate this strain with the type strain 280 ( = LMG 28095 = CIP 110700).

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2014-05-01
2024-03-28
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