An actinomycete strain, designated I12A-02593T, was isolated from a desert soil crust sample collected in the Shapotou region of Tengger Desert, north-west China. The isolate grew well on International Streptomyces Project (ISP) media 2, 3, 5 and 7, YS and Bennett’s agar; it produced spherical bodies and formed clumps on the aerial mycelia on ISP 5 agar plates. Chemotaxonomically, the strain contained meso-diaminopimelic acid as the diagnostic diamino acid, arabinose and galactose as the diagnostic sugars in whole-cell hydrolysates, MK-9(H4) as the sole isoprenoid quinone, and iso-C16 : 0, iso-C16 : 0 2-OH and iso-C16 : 1 H as the major cellular fatty acids, without mycolic acids. The profile of the phospholipids mainly comprised diphosphatidylglycerol, phosphatidylethanolamine and hydroxyphosphatidylethanolamine. The genomic DNA G+C content was 70.1 mol%. The 16S rRNA gene sequence of strain I12A-02593T exhibited 96.4–97.4 % similarities with members of the genus Actinophytocola. In the phylogenetic tree based on 16S rRNA gene sequences, the isolate formed a robust cluster with Actinophytocola oryzae NBRC 105245T, Aactinophytocola timorensis NBRC 105524T, Actinophytocola corallina NBRC 105525T, Actinophytocola burenkhanensis NBRC 105883Tand Actinophytocola xinjiangensis NBRC 106673T. DNA–DNA hybridization values between strain I12A-02593T and the five species of the genus Actinophytocola were all less than 70 %. On the basis of the polyphasic taxonomy evidence, a novel species of the genus Actinophytocola is proposed, with the name Actinophytocola gilvus sp. nov. The type strain is I12A-02593T ( = CPCC 203543T = DSM 45828T = NBRC 109453T = KCTC 29165T). An emended description of the genus Actinophytocola is also provided.
Published Online:
Funding
This study was supported by the:
National Infrastructure of Microbial Resources (NIMR)
National Natural Science Foundation of China (NSFC)
(Award 81173026 and 30970008)
National S&T Major Special Project on Major New Drug Innovation
(Award 2010ZX09401–403)
Special Fund for Health-scientific Research in the Public Interest
(Award 201002021)
CollinsM. D.,
PirouzT.,
GoodfellowM.,
MinnikinD. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol100, 221–230. [View Article][PubMed]
De LeyJ.,
CattoirH.,
ReynaertsA.(1970). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem12, 133–142. [View Article][PubMed]
GrothI.,
SchumannP.,
RaineyF. A.,
MartinK.,
SchuetzeB.,
AugstenK.(1997).Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol47, 1129–1133. [View Article][PubMed]
IndanandaC.,
MatsumotoA.,
InahashiY.,
TakahashiY.,
DuangmalK.,
ThamchaipenetA.(2010).Actinophytocola oryzae gen. nov., sp. nov., isolated from the roots of Thai glutinous rice plants, a new member of the family Pseudonocardiaceae
. . Int J Syst Evol Microbiol60, 1141–1146. [View Article][PubMed]
KelloggJ. A.,
BankertD. A.,
WithersG. S.,
SweimlerW.,
KiehnT. E.,
PfyfferG. E.(2001). Application of the Sherlock Mycobacteria Identification System using high-performance liquid chromatography in a clinical laboratory. . J Clin Microbiol39, 964–970. [View Article][PubMed]
KimuraM.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16, 111–120. [View Article][PubMed]
KroppenstedtR. M.(1985). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by
GoodfellowM.,
MinnikinD. E.
. New York:: Academic Press;.
LechevalierH.,
LechevalierM. P.(1965). Classification des actinomycètes aérobies basée sur leur morphologie et leur composition chimique. . Ann Inst Pasteur (Paris)108, 662–673. (in French).[PubMed]
LechevalierM. P.,
LechevalierH. A.(1980). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Special Publicationno. 6), pp. 227–291. Edited by
DietzA.,
ThayerD. W.
. Arlington, VA:: Society for Industrial Microbiology;.
LiW.-J.,
XuP.,
SchumannP.,
ZhangY.-Q.,
PukallR.,
XuL.-H.,
StackebrandtE.,
JiangC.-L.(2007).Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia
. . Int J Syst Evol Microbiol57, 1424–1428. [View Article][PubMed]
MarmurJ.,
DotyP.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol5, 109–118. [View Article][PubMed]
MinnikinD. E.,
O’DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods2, 233–241. [View Article]
SmibertR. M.,
KriegN. L.(1994). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
. Washington, DC:: American Society for Microbiology;.
StackebrandtE.,
GoebelB. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol44, 846–849. [View Article]