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Abstract

An actinomycete strain, designated I12A-02593, was isolated from a desert soil crust sample collected in the Shapotou region of Tengger Desert, north-west China. The isolate grew well on International Project (ISP) media 2, 3, 5 and 7, YS and Bennett’s agar; it produced spherical bodies and formed clumps on the aerial mycelia on ISP 5 agar plates. Chemotaxonomically, the strain contained -diaminopimelic acid as the diagnostic diamino acid, arabinose and galactose as the diagnostic sugars in whole-cell hydrolysates, MK-9(H) as the sole isoprenoid quinone, and iso-C, iso-C 2-OH and iso-C H as the major cellular fatty acids, without mycolic acids. The profile of the phospholipids mainly comprised diphosphatidylglycerol, phosphatidylethanolamine and hydroxyphosphatidylethanolamine. The genomic DNA G+C content was 70.1 mol%. The 16S rRNA gene sequence of strain I12A-02593 exhibited 96.4–97.4 % similarities with members of the genus . In the phylogenetic tree based on 16S rRNA gene sequences, the isolate formed a robust cluster with NBRC 105245, NBRC 105524, NBRC 105525, NBRC 105883and NBRC 106673. DNA–DNA hybridization values between strain I12A-02593 and the five species of the genus were all less than 70 %. On the basis of the polyphasic taxonomy evidence, a novel species of the genus is proposed, with the name sp. nov. The type strain is I12A-02593 ( = CPCC 203543 = DSM 45828 = NBRC 109453 = KCTC 29165). An emended description of the genus is also provided.

Funding
This study was supported by the:
  • , National Infrastructure of Microbial Resources (NIMR)
  • , National Natural Science Foundation of China (NSFC) , (Award 81173026 and 30970008)
  • , National S&T Major Special Project on Major New Drug Innovation , (Award 2010ZX09401–403)
  • , Special Fund for Health-scientific Research in the Public Interest , (Award 201002021)
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2014-09-01
2021-03-08
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