gen. nov., sp. nov., isolated from a hot spring Free

Abstract

A slightly thermophilic, Gram-staining-negative and strictly aerobic bacteria, designated strain YIM 78141, was isolated from a sediment sample collected at Hehua hot spring, Tengchong, Yunnan province, south-west China. Cells of the strain were short-rod-shaped and colonies were yellowish and circular. The strain grew at pH 6.0–10.0 (optimum, pH 8.0–9.0) and 10–55 °C (optimum, 40–50 °C). Phylogenetic analyses based on 16S rRNA gene sequence comparison demonstrated that strain YIM 78141 belongs to the family , and strain YIM 78141 also showed low levels of 16S rRNA gene sequence similarity (below 93.4 %) with all other genera in this family. The only quinone was ubiquinone 8 and the genomic DNA G+C content was 67.3 mol%. Major fatty acids (>5 %) were C C Cω7 and summed feature 3. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phospholipids of unknown structure containing aminoglycophospholipid and three unidentified polar lipids. On the basis of the morphological, physiological and biochemical characteristics as well as genotypic data, this strain should be classified as a representative of a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is YIM 78141 ( = BCRC 80650 = KCTC 32558 = DSM 27258).

Funding
This study was supported by the:
  • China Ministry of Science & Technology (MOST) (Award 2013DFA31980)
  • Yunnan Provincial Natural Science Foundation (Award 2013FA004)
  • Education Department of Henan Province of China (Award 2011A180025)
  • Henan Provincial Undergraduate Training Programs for Innovation and Entrepreneurship (Award 2011060)
  • National Science Foundation (Award OISE-0968421)
  • Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme
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2015-01-01
2024-03-29
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