1887

Abstract

A slightly thermophilic, Gram-staining-negative and strictly aerobic bacteria, designated strain YIM 78141, was isolated from a sediment sample collected at Hehua hot spring, Tengchong, Yunnan province, south-west China. Cells of the strain were short-rod-shaped and colonies were yellowish and circular. The strain grew at pH 6.0–10.0 (optimum, pH 8.0–9.0) and 10–55 °C (optimum, 40–50 °C). Phylogenetic analyses based on 16S rRNA gene sequence comparison demonstrated that strain YIM 78141 belongs to the family , and strain YIM 78141 also showed low levels of 16S rRNA gene sequence similarity (below 93.4 %) with all other genera in this family. The only quinone was ubiquinone 8 and the genomic DNA G+C content was 67.3 mol%. Major fatty acids (>5 %) were C C Cω7 and summed feature 3. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phospholipids of unknown structure containing aminoglycophospholipid and three unidentified polar lipids. On the basis of the morphological, physiological and biochemical characteristics as well as genotypic data, this strain should be classified as a representative of a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is YIM 78141 ( = BCRC 80650 = KCTC 32558 = DSM 27258).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.060996-0
2015-01-01
2019-11-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/1/214.html?itemId=/content/journal/ijsem/10.1099/ijs.0.060996-0&mimeType=html&fmt=ahah

References

  1. Cerny G.. ( 1978;). Studies on aminopeptidase for the distinction of Gram-negative from Gram-positive bacteria. . Appl Microbiol Biotechnol 5:, 113–122. [CrossRef]
    [Google Scholar]
  2. Chern L. L., Stackebrandt E., Lee S. F., Lee F. L., Chen J. K., Fu H. M.. ( 2004;). Chitinibacter tainanensis gen. nov., sp. nov., a chitin-degrading aerobe from soil in Taiwan. . Int J Syst Evol Microbiol 54:, 1387–1391. [CrossRef][PubMed]
    [Google Scholar]
  3. Collins M. D., Jones D.. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2,4-diaminobutyric acid. . J Appl Microbiol 48:, 459–470. [CrossRef]
    [Google Scholar]
  4. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in Actinomycetes and Corynebacteria.. J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  5. Cui X. L., Mao P. H., Zeng M., Li W. J., Zhang L. P., Xu L. H., Jiang C. L.. ( 2001;). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. . Int J Syst Evol Microbiol 51:, 357–363.[PubMed]
    [Google Scholar]
  6. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  9. Gillis M., Logan N. A.. ( 2005;). Genus IV. Chromobacterium Bergonzini 1881, 153AL. . In Bergey’s Mannual of Systematic Bacteriololgy, , 2nd edn., vol. 2, Part C, pp. 824–827. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M... New York:: Springer;. [CrossRef]
    [Google Scholar]
  10. Grimes D. J., Woese C. R., MacDonell M. T., Colwell R. R.. ( 1997;). Systematic study of the genus Vogesella gen. nov. and its type species, Vogesella indigofera comb. nov.. Int J Syst Bacteriol 47:, 19–27. [CrossRef][PubMed]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  12. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  13. Krieg N. R.. ( 2005;). Genus XIII. Vogesella Grimes, Woese, Macdonell and Colwell 1997, 25VP. . In Bergey’s Mannual of Systematic Bacteriololgy, , 2nd edn., vol. 2, Part C, pp. 858–859. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M... New York:: Springer;. [CrossRef]
    [Google Scholar]
  14. Kroppenstedt R. M.. ( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  15. Kwon S. W., Kim B. Y., Kim W. G., Yoo K. H., Yoo S. H., Son J. A., Weon H. Y.. ( 2008;). Paludibacterium yongneupense gen. nov., sp. nov., isolated from a wetland, Yongneup, in Korea. . Int J Syst Evol Microbiol 58:, 190–194. [CrossRef][PubMed]
    [Google Scholar]
  16. Lau H. T., Faryna J., Triplett E. W.. ( 2006;). Aquitalea magnusonii gen. nov., sp. nov., a novel Gram-negative bacterium isolated from a humic lake. . Int J Syst Evol Microbiol 56:, 867–871. [CrossRef][PubMed]
    [Google Scholar]
  17. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L.. ( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef][PubMed]
    [Google Scholar]
  18. Lin M. C., Chou J. H., Arun A. B., Young C. C., Chen W. M.. ( 2008;). Pseudogulbenkiania subflava gen. nov., sp. nov., isolated from a cold spring. . Int J Syst Evol Microbiol 58:, 2384–2388. [CrossRef][PubMed]
    [Google Scholar]
  19. Martin P. A. W., Gundersen-Rindal D., Blackburn M., Buyer J.. ( 2007;). Chromobacterium subtsugae sp. nov., a betaproteobacterium toxic to Colorado potato beetle and other insect pests. . Int J Syst Evol Microbiol 57:, 993–999. [CrossRef][PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  21. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  22. Patureau D., Godon J.-J., Dabert P., Bouchez T., Bernet N., Delgenes J. P., Moletta R.. ( 1998;). Microvirgula aerodenitrificans gen. nov., sp. nov., a new Gram-negative bacterium exhibiting co-respiration of oxygen and nitrogen oxides up to oxygen-saturated conditions. . Int J Syst Bacteriol 48:, 775–782. [CrossRef][PubMed]
    [Google Scholar]
  23. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  25. Vaz-Moreira I., Nobre M. F., Nunes O. C., Manaia C. M.. ( 2007;). Gulbenkiania mobilis gen. nov., sp. nov., isolated from treated municipal wastewater. . Int J Syst Evol Microbiol 57:, 1108–1112. [CrossRef][PubMed]
    [Google Scholar]
  26. Vela A. I., Collins M. D., Lawson P. A., García N., Domínguez L., Fernández-Garayzábal J. F.. ( 2005;). Uruburuella suis gen. nov., sp. nov., isolated from clinical specimens of pigs. . Int J Syst Evol Microbiol 55:, 643–647. [CrossRef][PubMed]
    [Google Scholar]
  27. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L.. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef][PubMed]
    [Google Scholar]
  28. Yuen K.-Y., Woo P. C. Y., Teng J. L. L., Leung K.-W., Wong M. K. M., Lau S. K. P.. ( 2001;). Laribacter hongkongensis gen. nov., sp. nov., a novel Gram-negative bacterium isolated from a cirrhotic patient with bacteremia and empyema. . J Clin Microbiol 39:, 4227–4232. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.060996-0
Loading
/content/journal/ijsem/10.1099/ijs.0.060996-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error