1887

Abstract

A novel pink-pigmented, facultatively methylotrophic strain, designated M6-76, was isolated from glacial foreland soil near Ny-Ålesund, Svalbard Archipelago, Norway. Cells of strain M6-76 were rod-shaped (0.4–0.7×0.8–2.0 µm), Gram-stain-negative, aerobic and motile by a single polar flagellum. Growth occurred at 4–28 °C (optimum 18 °C) and at pH 5–8 (optimum pH 7). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M6-76 belonged to the family . The 16S rRNA gene sequence of the novel strain showed 94.6 %, 94.0 % and 93.9 % sequence similarity to those of MR, ATCC 27886 and FaiI4, respectively. Cells could utilize methanol as the sole source of carbon and energy but not formate. The major respiratory quinone was Q-10. The polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and two unknown polar lipids. The predominant cellular fatty acids were summed feature 8 (Cω7 and/or Cω6), summed feature 3 (Cω6 and/or Cω7) and C. The DNA G+C content was 67 mol%. The polyphasic data presented in this study indicated that the isolate should be classified as representing a novel species of a new genus within the family . The name gen. nov., sp. nov. is therefore proposed for the isolate. The type strain of the type species is M6-76 ( = CCTCC AB 2013033 = KACC 17684).

Funding
This study was supported by the:
  • National Basic Research Program of China (973 Program) (Award 2011CB808800)
  • National Infrastructure of Natural Resources for Science and Technology Program of China (Award NIMR-2012-8)
  • National Natural Science Foundation of China (Award 364003)
  • State Oceanic Administration, PR China (Award 10/11YR06)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.060913-0
2014-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/5/1817.html?itemId=/content/journal/ijsem/10.1099/ijs.0.060913-0&mimeType=html&fmt=ahah

References

  1. Bousfield I. J., Green P. N. ( 1985 ). Reclassification of bacteria of the genus Protomonas Urakami and Komagata 1984 in the genus Methylobacterium (Patt, Cole, and Hanson) emend. Green and Bousfield 1983. . Int J Syst Bacteriol 35, 209. [View Article]
    [Google Scholar]
  2. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. . Int J Syst Evol Microbiol 50, 18611868.[PubMed]
    [Google Scholar]
  3. Doetsch R. N. ( 1981 ). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 2133. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  4. Dorsch M., Lane D., Stackebrandt E. ( 1992 ). Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences. . Int J Syst Bacteriol 42, 5863. [View Article] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  6. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  7. Garrity G. M., Holt J. G. ( 2001 ). The road map to the Manual . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., pp. 119166. Edited by Boone D. R., Castenholz R. W., Garrity G. M. . New York:: Springer;. [View Article]
    [Google Scholar]
  8. Green P. N. ( 2006 ). Methylobacterium . . In The Prokaryotes. A Handbook on the Biology of Bacteria, , 3rd edn., vol. 5, pp. 257265. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;.
    [Google Scholar]
  9. Green P. N., Bousfield I. J. ( 1982 ). A taxonomic study of some Gram-negative facultatively methylotrophic bacteria. . J Gen Microbiol 128, 623638. [View Article]
    [Google Scholar]
  10. Kanso S., Patel B. K. C. ( 2003 ). Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer. . Int J Syst Evol Microbiol 53, 401406. [View Article] [PubMed]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., Yi H., Won S., Chun J. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62, 716721. summed feature 8 contains C18 : 1ω7c and/or C18 : 1ω6c [CrossRef]
    [Google Scholar]
  12. Kovács N. ( 1956 ). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178, 703704. [View Article] [PubMed]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  14. Patt T. E., Cole G. C., Hanson R. S. ( 1976 ). Methylobacterium, a new genus of facultatively methylotrophic bacteria. . Int J Syst Bacteriol 26, 226229. [View Article]
    [Google Scholar]
  15. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. ( 1989 ). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  17. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
    [Google Scholar]
  18. Skerman V. B. D., McGowan V., Sneath P. H. A. ( 1980 ). Approved lists of bacterial names. . Int J Syst Bacteriol 30, 225420. [View Article] [PubMed]
    [Google Scholar]
  19. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  20. Tani A., Sahin N., Kimbara K. ( 2012 ). Methylobacterium gnaphalii sp. nov., isolated from leaves of Gnaphalium spicatum . . Int J Syst Evol Microbiol 62, 26022607. [View Article] [PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  22. Thomsen T. R., Blackall L. L., de Muro M. A., Nielsen J. L., Nielsen P. H. ( 2006 ). Meganema perideroedes gen. nov., sp. nov., a filamentous alphaproteobacterium from activated sludge. . Int J Syst Evol Microbiol 56, 18651868. [View Article] [PubMed]
    [Google Scholar]
  23. Tindall B. J. ( 1990 ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  24. Urakami T., Komagata K. ( 1984 ). Protomonas, a new genus of facultatively methylotrophic bacteria. . Int J Syst Bacteriol 34, 188201. [View Article]
    [Google Scholar]
  25. Urakami T., Araki H., Suzuki K.-I., Komagata K. ( 1993 ). Further studies of the genus Methylobacterium and description of Methylobacterium aminovorans sp. nov.. Int J Syst Bacteriol 43, 504513. [View Article]
    [Google Scholar]
  26. Ventosa A., Marquez M. C., Kocur M., Tindall B. J. ( 1993 ). Comparative study of “Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus . . Int J Syst Bacteriol 43, 245248. [View Article] [PubMed]
    [Google Scholar]
  27. Wang X., Sahr F., Xue T., Sun B. ( 2007 ). Methylobacterium salsuginis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 57, 16991703. [View Article] [PubMed]
    [Google Scholar]
  28. Weon H. Y., Kim B. Y., Joa J. H., Son J. A., Song M. H., Kwon S. W., Go S. J., Yoon S. H. ( 2008 ). Methylobacterium iners sp. nov. and Methylobacterium aerolatum sp. nov., isolated from air samples in Korea. . Int J Syst Evol Microbiol 58, 9396. [View Article] [PubMed]
    [Google Scholar]
  29. Weon H.-Y., Kwon S.-W., Son J.-A., Jo E.-H., Kim S.-J., Kim Y.-S., Kim B.-Y., Ka J.-O. ( 2010 ). Description of Microvirga aerophila sp. nov. and Microvirga aerilata sp. nov., isolated from air, reclassification of Balneimonas flocculans Takeda et al. 2004 as Microvirga flocculans comb. nov. and emended description of the genus Microvirga . . Int J Syst Evol Microbiol 60, 25962600. [View Article] [PubMed]
    [Google Scholar]
  30. Xie C. H., Yokota A. ( 2003 ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49, 345349. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.060913-0
Loading
/content/journal/ijsem/10.1099/ijs.0.060913-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error