1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_5

sp. nov., isolated from a plant stem Free

Abstract

A yellow-pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain JM-1070) was isolated as a presumptive endophyte from internal stem tissue of a healthy corn stem. Phylogenetic analyses based on the 16S rRNA gene sequence placed strain JM-1070 in the monophyletic cluster of the genus , with closest affiliation to the type strains of (98 % similarity) and (97.2 %). 16S rRNA gene sequence similarity to type strains of other species of the genus was 93.4–96.0 %. DNA–DNA hybridizations between strain JM-1070 and the type strains LMG 23497 and CCUG 63418 showed low relatedness values of 13 % (reciprocal 18 %) and 52 % (reciprocal 54.4 %). Major respiratory quinones were menaquinones MK-6 and MK-7. The predominant fatty acids (>15 %) were iso-C, iso-C 2-OH/Cω7 (measured as summed feature 3) and iso-C 3-OH. Several other iso-branched and hydroxylated fatty acids were detected. The polar lipid profile was composed of the major components phosphatidylethanolamine and an unidentified aminophospholipid. The polyamine pattern contained predominantly -homospermidine. Characterization by 16S rRNA gene sequencing, physiological parameters and polyamine, ubiquinone, polar lipid and fatty acid compositions revealed that strain JM-1070 represents a novel species of the genus . For this reason, we propose the name sp. nov., with the type strain JM-1070 ( = CIP 110694 = LMG 28078).

  • Published Online:
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.060848-0
2014-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/5/1736.html?itemId=/content/journal/ijsem/10.1099/ijs.0.060848-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H. J. ( 1996 ). Classification of bacteria isolated from a medieval wall painting. . J Biotechnol 47, 39–52. [View Article]
    [Google Scholar]
  2. An D. S., Yin C.-R., Lee S.-T. T., Cho C.-H. ( 2009 ). Mucilaginibacter daejeonensis sp. nov., isolated from dried rice straw. . Int J Syst Evol Microbiol 59, 1122–1125. [View Article] [PubMed]
    [Google Scholar]
  3. Baik K. S., Park S. C., Kim E. M., Lim C. H., Seong C. N. ( 2010 ). Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter . . Int J Syst Evol Microbiol 60, 134–139. [View Article] [PubMed]
    [Google Scholar]
  4. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. ( 1978 ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . . Proc Natl Acad Sci U S A 75, 4801–4805. [View Article] [PubMed]
    [Google Scholar]
  5. Busse H.-J., Auling G. ( 1988 ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . . Syst Appl Microbiol 11, 1–8. [View Article]
    [Google Scholar]
  6. Busse H.-J., Bunka S., Hensel A., Lubitz W. ( 1997 ). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. . Int J Syst Bacteriol 47, 698–708. [View Article]
    [Google Scholar]
  7. Cui C.-H., Choi T.-E., Yu H., Jin F., Lee S.-T., Kim S.-C., Im W.-T. ( 2011 ). Mucilaginibacter composti sp. nov., with ginsenoside converting activity, isolated from compost. . J Microbiol 49, 393–398. [View Article] [PubMed]
    [Google Scholar]
  8. Euzéby J. P. ( 1997 ). List of bacterial names with standing in nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47, 590–592. [View Article] [PubMed]
    [Google Scholar]
  9. Felsenstein J. ( 1985 ). Confidence limits of phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  10. Felsenstein J. ( 2005 ). phylip (Phylogeny Inference Package) version 3.6. . Distributed by the author. Department of Genome Sciences, University of Washington;, Seattle, USA:.
     [Google Scholar]
  11. Hamana K., Matsuzaki S. ( 1991 ). Polyamine distributions in the Flavobacterium-Cytophaga-Sphingobacterium complex. . Can J Microbiol 37, 885–888. [View Article] [PubMed]
    [Google Scholar]
  12. Hamana K., Nakagawa Y. ( 2001 ). Chemotaxonomic significance of polyamine distribution patterns in the Flavobacterium-Cytophaga complex and related genera. . Microbios 81, 135–145.
     [Google Scholar]
  13. Han S.-I., Lee H.-J., Lee H.-R., Kim K. K., Whang K.-S. ( 2012 ). Mucilaginibacter polysacchareus sp. nov., an exopolysaccharide-producing bacterial species isolated from the rhizoplane of the herb Angelica sinensis . . Int J Syst Evol Microbiol 62, 632–637. [View Article] [PubMed]
    [Google Scholar]
  14. Hwang Y. M., Baik K. S., Seong C. N. ( 2014 ). Mucilaginibacter defluvii sp. nov., isolated from a dye wastewater treatment facility. . Int J Syst Evol Microbiol 64, 565–571. [View Article] [PubMed]
    [Google Scholar]
  15. Jeon Y., Lee S.-S., Chung B. S., Kim J. M., Bae J.-W., Park S. K., Jeon C. O. ( 2009 ). Mucilaginibacter oryzae sp. nov., isolated from soil of a rice paddy. . Int J Syst Evol Microbiol 59, 1451–1454. [View Article] [PubMed]
    [Google Scholar]
  16. Jiang F., Dai J., Wang Y., Xue X., Xu M., Guo Y., Li W., Fang C., Peng F. ( 2012 ). Mucilaginibacter soli sp. nov., isolated from Arctic tundra soil. . Int J Syst Evol Microbiol 62, 1630–1635. [View Article] [PubMed]
    [Google Scholar]
  17. Joung Y., Joh K. ( 2011 ). Mucilaginibacter myungsuensis sp. nov., isolated from a mesotrophic artificial lake. . Int J Syst Evol Microbiol 61, 1506–1510. [View Article] [PubMed]
    [Google Scholar]
  18. Joung Y., Kim H., Kang H., Lee B.-I., Ahn T.-S., Joh K. ( 2014 ). Mucilaginibacter soyangensis sp. nov., isolated from a lake. . Int J Syst Evol Microbiol 64, 413–419. [View Article] [PubMed]
    [Google Scholar]
  19. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  20. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 989–1005. [View Article]
    [Google Scholar]
  21. Kämpfer P., Steiof M., Dott W. ( 1991 ). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. . Microb Ecol 21, 227–251. [View Article] [PubMed]
    [Google Scholar]
  22. Kang S. J., Jung Y. T., Oh K. H., Oh T. K., Yoon J. H. ( 2011 ). Mucilaginibacter boryungensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 61, 1549–1553. [View Article] [PubMed]
    [Google Scholar]
  23. Kang C.-H., Jung Y.-T., Yoon J.-H. ( 2013 ). Mucilaginibacter sabulilitoris sp. nov., isolated from marine sand in a firth. . Int J Syst Evol Microbiol 63, 2865–2871. [View Article] [PubMed]
    [Google Scholar]
  24. Khan H., Chung E. J., Jeon C. O., Chung Y. R. ( 2013a ). Mucilaginibacter gynuensis sp. nov., isolated from rotten wood. . Int J Syst Evol Microbiol 63, 3225–3231. [View Article] [PubMed]
    [Google Scholar]
  25. Khan H., Chung E. J., Kang D. Y., Jeon C. O., Chung Y. R. ( 2013b ). Mucilaginibacter jinjuensis sp. nov., with xylan-degrading activity.. Int J Syst Evol Microbiol 63, 1267–1272. [View Article] [PubMed]
    [Google Scholar]
  26. Kim M. K., Na J.-R., Cho D. H., Soung N.-K., Yang D.-C. ( 2007 ). Parapedobacter koreensis gen. nov., sp. nov.. Int J Syst Evol Microbiol 57, 1336–1341. [View Article] [PubMed]
    [Google Scholar]
  27. Kim B. C., Lee K. H., Kim M. N., Lee J., Shin K. S. ( 2010a ). Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum . . FEMS Microbiol Lett 309, 130–135.[PubMed]
    [Google Scholar]
  28. Kim S.-J., Weon H.-Y., Kim Y.-S., Yoo S.-H., Kim B.-Y., Anandham R., Kwon S.-W. ( 2010b ). Parapedobacter luteus sp. nov. and Parapedobacter composti sp. nov., isolated from cotton waste compost. . Int J Syst Evol Microbiol 60, 1849–1853. [View Article] [PubMed]
    [Google Scholar]
  29. Kim B.-C., Poo H., Lee K. H., Kim M. N., Kwon O.-Y., Shin K.-S. ( 2012a ). Mucilaginibacter angelicae sp. nov., a novel bacterium isolated from the rhizosphere of Angelica polymorpha Maxim. . Int J Syst Evol Microbiol 62, 55–60. [View Article] [PubMed]
    [Google Scholar]
  30. Kim J.-H., Kang S.-J., Jung Y.-T., Oh T.-K., Yoon J.-H. ( 2012b ). Mucilaginibacter lutimaris sp. nov., isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 62, 515–519. [View Article] [PubMed]
    [Google Scholar]
  31. Kim J. K., Choi T. E., Liu Q. M., Park H. Y., Yi T. H., Yoon M. H., Kim S. C., Im W. T. ( 2013 ). Mucilaginibacter ginsenosidivorax sp. nov., with ginsenoside converting activity isolated from sediment. . J Microbiol 51, 394–399. [View Article] [PubMed]
    [Google Scholar]
  32. Lee H. R., Han S. I., Rhee K. H., Whang K. S. ( 2013 ). Mucilaginibacter herbaticus sp. nov., isolated from the rhizosphere of the medicinal plant Angelica sinensis . . Int J Syst Evol Microbiol 63, 2787–2793. [View Article] [PubMed]
    [Google Scholar]
  33. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 1363–1371. [View Article] [PubMed]
    [Google Scholar]
  34. Luo X., Zhang L., Dai J., Liu M., Zhang K., An H., Fang C. ( 2009 ). Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil. . Int J Syst Evol Microbiol 59, 1447–1450. [View Article] [PubMed]
    [Google Scholar]
  35. Madhaiyan M., Poonguzhali S., Lee J. S., Senthilkumar M., Lee K. C., Sundaram S. ( 2010 ). Mucilaginibacter gossypii sp. nov. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils. . Int J Syst Evol Microbiol 60, 2451–2457. [View Article] [PubMed]
    [Google Scholar]
  36. Männistö M. K., Tiirola M., McConnell J., Häggblom M. M. ( 2010 ). Mucilaginibacter frigoritolerans sp. nov., Mucilaginibacter lappiensis sp. nov. and Mucilaginibacter mallensis sp. nov., isolated from soil and lichen samples. . Int J Syst Evol Microbiol 60, 2849–2856. [View Article] [PubMed]
    [Google Scholar]
  37. Pankratov T. A., Tindall B. J., Liesack W., Dedysh S. N. ( 2007 ). Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. . Int J Syst Evol Microbiol 57, 2349–2354. [View Article] [PubMed]
    [Google Scholar]
  38. Pitcher D. G., Saunders N. A., Owen R. J. ( 1989 ). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. . Lett Appl Microbiol 8, 151–156. [View Article]
    [Google Scholar]
  39. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. ( 2007 ). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb . . Nucleic Acids Res 35, 7188–7196. [View Article] [PubMed]
    [Google Scholar]
  40. Pruesse E., Peplies J., Glöckner F. O. ( 2012 ). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28, 1823–1829. [View Article] [PubMed]
    [Google Scholar]
  41. Schleifer K. P. ( 1985 ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18, 123–156. [View Article]
    [Google Scholar]
  42. Stamatakis A. ( 2006 ). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22, 2688–2690. [View Article] [PubMed]
    [Google Scholar]
  43. Stolz A., Busse H.-J., Kämpfer P. ( 2007 ). Pseudomonas knackmussii sp. nov.. Int J Syst Evol Microbiol 57, 572–576. [View Article] [PubMed]
    [Google Scholar]
  44. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128–130. [View Article]
    [Google Scholar]
  45. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199–202. [View Article]
    [Google Scholar]
  46. Urai M., Aizawa T., Nakagawa Y., Nakajima M., Sunairi M. ( 2008 ). Mucilaginibacter kameinonensis sp., nov., isolated from garden soil. . Int J Syst Evol Microbiol 58, 2046–2050. [View Article] [PubMed]
    [Google Scholar]
  47. Yarza P., Richter M., Peplies J., Euzéby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R. ( 2008 ). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31, 241–250. [View Article] [PubMed]
    [Google Scholar]
  48. Yoon J. H., Kang S. J., Park S., Oh T. K. ( 2012 ). Mucilaginibacter litoreus sp. nov., isolated from marine sand. . Int J Syst Evol Microbiol 62, 2822–2827. [View Article] [PubMed]
    [Google Scholar]
  49. Yoon J. H., Park S., Jung Y. T. ( 2013 ). Mucilaginibacter calamicampi sp. nov., a member of the family Sphingobacteriaceae isolated from soil at a field of reeds. . Antonie van Leeuwenhoek 104, 37–45. [View Article] [PubMed]
    [Google Scholar]
  50. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. ( 1998 ). Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov.. Int J Syst Bacteriol 48, 179–186. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.060848-0
Loading
Mucilaginibacter auburnensis sp. nov., isolated from a plant stem
Int J Syst Evol Microbiol 64, 1736 (2014); https://doi.org/10.1099/ijs.0.060848-0
/content/journal/ijsem/10.1099/ijs.0.060848-0
/content/journal/ijsem/10.1099/ijs.0.060848-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error