1887

Abstract

A yellow-pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain JM-1070) was isolated as a presumptive endophyte from internal stem tissue of a healthy corn stem. Phylogenetic analyses based on the 16S rRNA gene sequence placed strain JM-1070 in the monophyletic cluster of the genus , with closest affiliation to the type strains of (98 % similarity) and (97.2 %). 16S rRNA gene sequence similarity to type strains of other species of the genus was 93.4–96.0 %. DNA–DNA hybridizations between strain JM-1070 and the type strains LMG 23497 and CCUG 63418 showed low relatedness values of 13 % (reciprocal 18 %) and 52 % (reciprocal 54.4 %). Major respiratory quinones were menaquinones MK-6 and MK-7. The predominant fatty acids (>15 %) were iso-C, iso-C 2-OH/Cω7 (measured as summed feature 3) and iso-C 3-OH. Several other iso-branched and hydroxylated fatty acids were detected. The polar lipid profile was composed of the major components phosphatidylethanolamine and an unidentified aminophospholipid. The polyamine pattern contained predominantly -homospermidine. Characterization by 16S rRNA gene sequencing, physiological parameters and polyamine, ubiquinone, polar lipid and fatty acid compositions revealed that strain JM-1070 represents a novel species of the genus . For this reason, we propose the name sp. nov., with the type strain JM-1070 ( = CIP 110694 = LMG 28078).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.060848-0
2014-05-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/5/1736.html?itemId=/content/journal/ijsem/10.1099/ijs.0.060848-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H. J.. ( 1996;). Classification of bacteria isolated from a medieval wall painting. . J Biotechnol 47:, 39–52. [CrossRef]
    [Google Scholar]
  2. An D. S., Yin C.-R., Lee S.-T. T., Cho C.-H.. ( 2009;). Mucilaginibacter daejeonensis sp. nov., isolated from dried rice straw. . Int J Syst Evol Microbiol 59:, 1122–1125. [CrossRef][PubMed]
    [Google Scholar]
  3. Baik K. S., Park S. C., Kim E. M., Lim C. H., Seong C. N.. ( 2010;). Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter. . Int J Syst Evol Microbiol 60:, 134–139. [CrossRef][PubMed]
    [Google Scholar]
  4. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F.. ( 1978;). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. . Proc Natl Acad Sci U S A 75:, 4801–4805. [CrossRef][PubMed]
    [Google Scholar]
  5. Busse H.-J., Auling G.. ( 1988;). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. . Syst Appl Microbiol 11:, 1–8. [CrossRef]
    [Google Scholar]
  6. Busse H.-J., Bunka S., Hensel A., Lubitz W.. ( 1997;). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. . Int J Syst Bacteriol 47:, 698–708. [CrossRef]
    [Google Scholar]
  7. Cui C.-H., Choi T.-E., Yu H., Jin F., Lee S.-T., Kim S.-C., Im W.-T.. ( 2011;). Mucilaginibacter composti sp. nov., with ginsenoside converting activity, isolated from compost. . J Microbiol 49:, 393–398. [CrossRef][PubMed]
    [Google Scholar]
  8. Euzéby J. P.. ( 1997;). List of bacterial names with standing in nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47:, 590–592. [CrossRef][PubMed]
    [Google Scholar]
  9. Felsenstein J.. ( 1985;). Confidence limits of phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  10. Felsenstein J.. ( 2005;). phylip (Phylogeny Inference Package) version 3.6. . Distributed by the author. Department of Genome Sciences, University of Washington;, Seattle, USA:.
  11. Hamana K., Matsuzaki S.. ( 1991;). Polyamine distributions in the Flavobacterium-Cytophaga-Sphingobacterium complex. . Can J Microbiol 37:, 885–888. [CrossRef][PubMed]
    [Google Scholar]
  12. Hamana K., Nakagawa Y.. ( 2001;). Chemotaxonomic significance of polyamine distribution patterns in the Flavobacterium-Cytophaga complex and related genera. . Microbios 81:, 135–145.
    [Google Scholar]
  13. Han S.-I., Lee H.-J., Lee H.-R., Kim K. K., Whang K.-S.. ( 2012;). Mucilaginibacter polysacchareus sp. nov., an exopolysaccharide-producing bacterial species isolated from the rhizoplane of the herb Angelica sinensis. . Int J Syst Evol Microbiol 62:, 632–637. [CrossRef][PubMed]
    [Google Scholar]
  14. Hwang Y. M., Baik K. S., Seong C. N.. ( 2014;). Mucilaginibacter defluvii sp. nov., isolated from a dye wastewater treatment facility. . Int J Syst Evol Microbiol 64:, 565–571. [CrossRef][PubMed]
    [Google Scholar]
  15. Jeon Y., Lee S.-S., Chung B. S., Kim J. M., Bae J.-W., Park S. K., Jeon C. O.. ( 2009;). Mucilaginibacter oryzae sp. nov., isolated from soil of a rice paddy. . Int J Syst Evol Microbiol 59:, 1451–1454. [CrossRef][PubMed]
    [Google Scholar]
  16. Jiang F., Dai J., Wang Y., Xue X., Xu M., Guo Y., Li W., Fang C., Peng F.. ( 2012;). Mucilaginibacter soli sp. nov., isolated from Arctic tundra soil. . Int J Syst Evol Microbiol 62:, 1630–1635. [CrossRef][PubMed]
    [Google Scholar]
  17. Joung Y., Joh K.. ( 2011;). Mucilaginibacter myungsuensis sp. nov., isolated from a mesotrophic artificial lake. . Int J Syst Evol Microbiol 61:, 1506–1510. [CrossRef][PubMed]
    [Google Scholar]
  18. Joung Y., Kim H., Kang H., Lee B.-I., Ahn T.-S., Joh K.. ( 2014;). Mucilaginibacter soyangensis sp. nov., isolated from a lake. . Int J Syst Evol Microbiol 64:, 413–419. [CrossRef][PubMed]
    [Google Scholar]
  19. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N... New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  20. Kämpfer P., Kroppenstedt R. M.. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  21. Kämpfer P., Steiof M., Dott W.. ( 1991;). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. . Microb Ecol 21:, 227–251. [CrossRef][PubMed]
    [Google Scholar]
  22. Kang S. J., Jung Y. T., Oh K. H., Oh T. K., Yoon J. H.. ( 2011;). Mucilaginibacter boryungensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 61:, 1549–1553. [CrossRef][PubMed]
    [Google Scholar]
  23. Kang C.-H., Jung Y.-T., Yoon J.-H.. ( 2013;). Mucilaginibacter sabulilitoris sp. nov., isolated from marine sand in a firth. . Int J Syst Evol Microbiol 63:, 2865–2871. [CrossRef][PubMed]
    [Google Scholar]
  24. Khan H., Chung E. J., Jeon C. O., Chung Y. R.. ( 2013a;). Mucilaginibacter gynuensis sp. nov., isolated from rotten wood. . Int J Syst Evol Microbiol 63:, 3225–3231. [CrossRef][PubMed]
    [Google Scholar]
  25. Khan H., Chung E. J., Kang D. Y., Jeon C. O., Chung Y. R.. ( 2013b;). Mucilaginibacter jinjuensis sp. nov., with xylan-degrading activity.. Int J Syst Evol Microbiol 63:, 1267–1272. [CrossRef][PubMed]
    [Google Scholar]
  26. Kim M. K., Na J.-R., Cho D. H., Soung N.-K., Yang D.-C.. ( 2007;). Parapedobacter koreensis gen. nov., sp. nov.. Int J Syst Evol Microbiol 57:, 1336–1341. [CrossRef][PubMed]
    [Google Scholar]
  27. Kim B. C., Lee K. H., Kim M. N., Lee J., Shin K. S.. ( 2010a;). Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum. . FEMS Microbiol Lett 309:, 130–135.[PubMed]
    [Google Scholar]
  28. Kim S.-J., Weon H.-Y., Kim Y.-S., Yoo S.-H., Kim B.-Y., Anandham R., Kwon S.-W.. ( 2010b;). Parapedobacter luteus sp. nov. and Parapedobacter composti sp. nov., isolated from cotton waste compost. . Int J Syst Evol Microbiol 60:, 1849–1853. [CrossRef][PubMed]
    [Google Scholar]
  29. Kim B.-C., Poo H., Lee K. H., Kim M. N., Kwon O.-Y., Shin K.-S.. ( 2012a;). Mucilaginibacter angelicae sp. nov., a novel bacterium isolated from the rhizosphere of Angelica polymorpha Maxim. . Int J Syst Evol Microbiol 62:, 55–60. [CrossRef][PubMed]
    [Google Scholar]
  30. Kim J.-H., Kang S.-J., Jung Y.-T., Oh T.-K., Yoon J.-H.. ( 2012b;). Mucilaginibacter lutimaris sp. nov., isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 62:, 515–519. [CrossRef][PubMed]
    [Google Scholar]
  31. Kim J. K., Choi T. E., Liu Q. M., Park H. Y., Yi T. H., Yoon M. H., Kim S. C., Im W. T.. ( 2013;). Mucilaginibacter ginsenosidivorax sp. nov., with ginsenoside converting activity isolated from sediment. . J Microbiol 51:, 394–399. [CrossRef][PubMed]
    [Google Scholar]
  32. Lee H. R., Han S. I., Rhee K. H., Whang K. S.. ( 2013;). Mucilaginibacter herbaticus sp. nov., isolated from the rhizosphere of the medicinal plant Angelica sinensis. . Int J Syst Evol Microbiol 63:, 2787–2793. [CrossRef][PubMed]
    [Google Scholar]
  33. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S.. & other authors ( 2004;). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef][PubMed]
    [Google Scholar]
  34. Luo X., Zhang L., Dai J., Liu M., Zhang K., An H., Fang C.. ( 2009;). Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil. . Int J Syst Evol Microbiol 59:, 1447–1450. [CrossRef][PubMed]
    [Google Scholar]
  35. Madhaiyan M., Poonguzhali S., Lee J. S., Senthilkumar M., Lee K. C., Sundaram S.. ( 2010;). Mucilaginibacter gossypii sp. nov. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils. . Int J Syst Evol Microbiol 60:, 2451–2457. [CrossRef][PubMed]
    [Google Scholar]
  36. Männistö M. K., Tiirola M., McConnell J., Häggblom M. M.. ( 2010;). Mucilaginibacter frigoritolerans sp. nov., Mucilaginibacter lappiensis sp. nov. and Mucilaginibacter mallensis sp. nov., isolated from soil and lichen samples. . Int J Syst Evol Microbiol 60:, 2849–2856. [CrossRef][PubMed]
    [Google Scholar]
  37. Pankratov T. A., Tindall B. J., Liesack W., Dedysh S. N.. ( 2007;). Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. . Int J Syst Evol Microbiol 57:, 2349–2354. [CrossRef][PubMed]
    [Google Scholar]
  38. Pitcher D. G., Saunders N. A., Owen R. J.. ( 1989;). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. . Lett Appl Microbiol 8:, 151–156. [CrossRef]
    [Google Scholar]
  39. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O.. ( 2007;). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. . Nucleic Acids Res 35:, 7188–7196. [CrossRef][PubMed]
    [Google Scholar]
  40. Pruesse E., Peplies J., Glöckner F. O.. ( 2012;). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28:, 1823–1829. [CrossRef][PubMed]
    [Google Scholar]
  41. Schleifer K. P.. ( 1985;). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18:, 123–156. [CrossRef]
    [Google Scholar]
  42. Stamatakis A.. ( 2006;). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22:, 2688–2690. [CrossRef][PubMed]
    [Google Scholar]
  43. Stolz A., Busse H.-J., Kämpfer P.. ( 2007;). Pseudomonas knackmussii sp. nov.. Int J Syst Evol Microbiol 57:, 572–576. [CrossRef][PubMed]
    [Google Scholar]
  44. Tindall B. J.. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  45. Tindall B. J.. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  46. Urai M., Aizawa T., Nakagawa Y., Nakajima M., Sunairi M.. ( 2008;). Mucilaginibacter kameinonensis sp., nov., isolated from garden soil. . Int J Syst Evol Microbiol 58:, 2046–2050. [CrossRef][PubMed]
    [Google Scholar]
  47. Yarza P., Richter M., Peplies J., Euzéby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R.. ( 2008;). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31:, 241–250. [CrossRef][PubMed]
    [Google Scholar]
  48. Yoon J. H., Kang S. J., Park S., Oh T. K.. ( 2012;). Mucilaginibacter litoreus sp. nov., isolated from marine sand. . Int J Syst Evol Microbiol 62:, 2822–2827. [CrossRef][PubMed]
    [Google Scholar]
  49. Yoon J. H., Park S., Jung Y. T.. ( 2013;). Mucilaginibacter calamicampi sp. nov., a member of the family Sphingobacteriaceae isolated from soil at a field of reeds. . Antonie van Leeuwenhoek 104:, 37–45. [CrossRef][PubMed]
    [Google Scholar]
  50. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R.. ( 1998;). Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov.. Int J Syst Bacteriol 48:, 179–186. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.060848-0
Loading
/content/journal/ijsem/10.1099/ijs.0.060848-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error