1887

Abstract

A yellow-pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium (strain JM-1070) was isolated as a presumptive endophyte from internal stem tissue of a healthy corn stem. Phylogenetic analyses based on the 16S rRNA gene sequence placed strain JM-1070 in the monophyletic cluster of the genus , with closest affiliation to the type strains of (98 % similarity) and (97.2 %). 16S rRNA gene sequence similarity to type strains of other species of the genus was 93.4–96.0 %. DNA–DNA hybridizations between strain JM-1070 and the type strains LMG 23497 and CCUG 63418 showed low relatedness values of 13 % (reciprocal 18 %) and 52 % (reciprocal 54.4 %). Major respiratory quinones were menaquinones MK-6 and MK-7. The predominant fatty acids (>15 %) were iso-C, iso-C 2-OH/Cω7 (measured as summed feature 3) and iso-C 3-OH. Several other iso-branched and hydroxylated fatty acids were detected. The polar lipid profile was composed of the major components phosphatidylethanolamine and an unidentified aminophospholipid. The polyamine pattern contained predominantly -homospermidine. Characterization by 16S rRNA gene sequencing, physiological parameters and polyamine, ubiquinone, polar lipid and fatty acid compositions revealed that strain JM-1070 represents a novel species of the genus . For this reason, we propose the name sp. nov., with the type strain JM-1070 ( = CIP 110694 = LMG 28078).

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2014-05-01
2020-07-09
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