RT Journal Article SR Electronic(1) A1 Kämpfer, Peter A1 Glaeser, Stefanie P. A1 McInroy, John A. A1 Busse, Hans-JürgenYR 2014 T1 Cohnellarhizosphaerae sp. nov., isolated from the rhizosphere environment of Zea mays JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_5 SP 1811 OP 1816 DO https://doi.org/10.1099/ijs.0.060814-0 PB Microbiology Society, SN 1466-5034, AB A Gram-staining-positive, aerobic, non-endospore forming organism, isolated as a seed endophyte (colonizing the internal healthy tissue of plant seed) of sweet corn (Zea mays), strain CSE-5610T, was studied for its taxonomic allocation. On the basis of 16S rRNA gene sequence comparisons, strain CSE-5610T was grouped into the genus Cohnella , most closely related to Cohnella ginsengisoli GR21-5T (98.1 %) and ‘Cohnella plantaginis’ YN-83 (97.5 %). The 16S rRNA gene sequence similarity to other members of the genus Cohnella was <96.6 %. DNA–DNA hybridization of strain CSE-5610T with C. ginsengisoli DSM 18997T and ‘C. plantaginis’ DSM 25424 was 58 % (reciprocal 24 %) and 30 % (reciprocal 27 %), respectively. The fatty acid profile from whole cell hydrolysates supported the allocation of the strain to the genus Cohnella ; iso- and anteiso-branched fatty acids were found as major compounds. meso-Diaminopimelic acid was identified as the cell-wall diamino acid. The quinone system consisted predominantly of menaquinone MK-7. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, a phospholipid and minor amounts of two polar lipids. In the polyamine pattern, spermidine was the major polyamine. The G+C content of the genomic DNA was 60 mol%. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain CSE-5610T from the two closely related strains. Hence, CSE-5610T represents a novel species of the genus Cohnella , for which we propose the name Cohnella rhizosphaerae sp. nov., with CSE-5610T ( = LMG 28080T = CIP 110695T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.060814-0