1887

Abstract

A Gram-staining-positive, aerobic, non-endospore forming organism, isolated as a seed endophyte (colonizing the internal healthy tissue of plant seed) of sweet corn (), strain CSE-5610, was studied for its taxonomic allocation. On the basis of 16S rRNA gene sequence comparisons, strain CSE-5610 was grouped into the genus , most closely related to GR21-5 (98.1 %) and ‘’ YN-83 (97.5 %). The 16S rRNA gene sequence similarity to other members of the genus was <96.6 %. DNA–DNA hybridization of strain CSE-5610 with DSM 18997 and ‘’ DSM 25424 was 58 % (reciprocal 24 %) and 30 % (reciprocal 27 %), respectively. The fatty acid profile from whole cell hydrolysates supported the allocation of the strain to the genus ; iso- and anteiso-branched fatty acids were found as major compounds. -Diaminopimelic acid was identified as the cell-wall diamino acid. The quinone system consisted predominantly of menaquinone MK-7. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, a phospholipid and minor amounts of two polar lipids. In the polyamine pattern, spermidine was the major polyamine. The G+C content of the genomic DNA was 60 mol%. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain CSE-5610 from the two closely related strains. Hence, CSE-5610 represents a novel species of the genus , for which we propose the name sp. nov., with CSE-5610 ( = LMG 28080 = CIP 110695) as the type strain.

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2014-05-01
2019-12-07
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