1887

Abstract

A novel bacterium, designated strain WP70, was isolated from the gut of a comb pen shell () collected from the southern sea of Yeosu in Korea. The isolate was Gram-stain-negative, aerobic, non-motile and rod-shaped. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain WP70 belonged to the genus . The highest level of sequence similarity (98.4 %) was shared with MKT110. Optimal growth occurred in 2 % (w/v) NaCl at 30 °C and at pH 7. The major cellular fatty acids were summed feature 3 (Cω7 and/or Cω6), summed feature 8 (Cω7 and/or Cω6) and C. The main respiratory quinone was Q-9. The polar lipids comprised phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, three unidentified phospholipids, an unidentified aminolipid, an unidentified aminophospholipid and an unidentified lipid. The genomic DNA G+C content was 50.5 mol% and DNA–DNA hybridization values indicated <11 % genomic relatedness to the closest species. Physiological, biochemical, chemotaxonomic and genotypic analyses indicated that strain WP70 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WP70 ( = KACC 17474 = JCM 19190).

Funding
This study was supported by the:
  • Mid-career Researcher Program (Award 2012-0008806)
  • National Research Foundation of Korea
  • National Institute of Biological Resources (NIBR)
  • Ministry of Environment (MOE) of the Republic of Korea (Award 2013-02-001)
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2014-07-01
2021-04-14
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