gen. nov., sp. nov., a lipolytic bacterium isolated from a tidal flat Free

Abstract

A Gram-stain-negative, non-motile, aerobic, curved-to-spiral-rod-shaped bacterium, designated AH-MY2, was isolated from a tidal flat on Aphae island in the sea to the south-west of South Korea, and its taxonomic position was investigated using a polyphasic taxonomic approach. Strain AH-MY2 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain AH-MY2 clustered with the type strain of and that this cluster joined the clade comprising the type strains of species of the genus . Strain AH-MY2 exhibited 16S rRNA gene sequence similarity values of 90.6 % to the type strain of and of less than 91.0 % to the type strains of other species with validly published names. Strain AH-MY2 contained Q-10 as the predominant ubiquinone and Cω7 as the major fatty acid. The major polar lipids detected in strain AH-MY2 were phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminolipids and one unidentified glycolipid. The DNA G+C content of strain AH-MY2 was 56.0 mol%. The phylogenetic data and differential chemotaxonomic and other phenotypic properties revealed that strain AH-MY2 represented a novel genus and species within the family of the class , for which the name gen. nov., sp. nov. is proposed. The type strain of is AH-MY2 ( = KCTC 32577 = CECT 8488).

Funding
This study was supported by the:
  • National Research Foundation (NRF)
  • Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea (Award 2012R1A2A2A01047369)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.060772-0
2014-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/6/1841.html?itemId=/content/journal/ijsem/10.1099/ijs.0.060772-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. (editors) ( 1993 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  2. Baumann P., Baumann L. ( 1981 ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 13021331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 19972006. [View Article] [PubMed]
    [Google Scholar]
  4. Buchan A., González J. M., Moran M. A. ( 2005 ). Overview of the marine roseobacter lineage. . Appl Environ Microbiol 71, 56655677. [View Article] [PubMed]
    [Google Scholar]
  5. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. ( 1957 ). Kinetic studies of pigment synthesis by non-sulfur purple bacteria. . J Cell Physiol 49, 2568. [View Article] [PubMed]
    [Google Scholar]
  6. Garrity G. M., Bell J. A., Lilburn T. ( 2005 ). Family I. Rhodobacteracea fam. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, p. 161. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;.
    [Google Scholar]
  7. Garrity G. M., Bell J. A., Lilburn T. ( 2006 ). Rhodobacteracea fam. nov. In List of new names and new combinations previously effectively, but not validly, published. Validation List no. 107. . Int J Syst Evol Microbiol 56, 16. [View Article] [PubMed]
    [Google Scholar]
  8. Hunter I. S. ( 1985 ). Gene cloning in Streptomyces . . In DNA Cloning, vol. 2, p. 32. Edited by Glover D. M. . Oxford:: IRL Press;.
    [Google Scholar]
  9. Jung Y.-T., Kim B.-H., Oh T.-K., Yoon J.-H. ( 2010 ). Pseudoruegeria lutimaris sp. nov., isolated from a tidal flat sediment, and emended description of the genus Pseudoruegeria . . Int J Syst Evol Microbiol 60, 11771181. [View Article] [PubMed]
    [Google Scholar]
  10. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  11. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  12. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. ( 1977 ). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27, 104117. [View Article]
    [Google Scholar]
  13. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  14. Rappé M. S., Vergin K., Giovannoni S. J. ( 2000 ). Phylogenetic comparisons of a coastal bacterioplankton community with its counterparts in open ocean and freshwater systems. . FEMS Microbiol Ecol 33, 219232. [View Article] [PubMed]
    [Google Scholar]
  15. Sakane T., Yokota A. ( 1994 ). Chemotaxonomic investigation of heterotrophic, aerobic and microaerophilic spirilla, the genera Aquaspirillum, Magnetospirillum, and Oceanospirillum . . Syst Appl Microbiol 17, 128134. [View Article]
    [Google Scholar]
  16. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE:: MIDI. Inc;.
    [Google Scholar]
  17. Satomi M., Kimura B., Hamada T., Harayama S., Fujii T. ( 2002 ). Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum, description of Pseudospirillum gen. nov., Oceanobacter gen. nov. and Terasakiella gen. nov. and transfer of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. nov.. Int J Syst Evol Microbiol 52, 739747. [View Article] [PubMed]
    [Google Scholar]
  18. Staley J. T. ( 1968 ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95, 19211942.[PubMed]
    [Google Scholar]
  19. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  20. Terasaki Y. ( 1979 ). Transfer of five species and two subspecies of Spirillum to other genera (Aquaspirillum and Oceanospirillum), with emended descriptions of the species and subspecies. . Int J Syst Bacteriol 29, 130144. [View Article]
    [Google Scholar]
  21. Yoon J.-H., Lee S. T., Park Y.-H. ( 1998 ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48, 187194. [View Article] [PubMed]
    [Google Scholar]
  22. Yoon J.-H., Kang K. H., Park Y.-H. ( 2003 ). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 53, 449454. [View Article] [PubMed]
    [Google Scholar]
  23. Yoon J.-H., Kang S.-J., Lee J.-S., Oh T.-K. ( 2009a ). Lutimaribacter saemankumensis gen. nov., sp. nov., isolated from a tidal flat of the Yellow Sea. . Int J Syst Evol Microbiol 59, 4852. [View Article] [PubMed]
    [Google Scholar]
  24. Yoon J.-H., Kang S.-J., Lee S.-Y., Oh K.-H., Oh T.-K. ( 2009b ). Seohaeicola saemankumensis gen. nov., sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 59, 26752679. [View Article] [PubMed]
    [Google Scholar]
  25. Yoon J.-H., Kang S.-J., Jung Y.-T., Oh T.-K. ( 2010 ). Gaetbulicola byunsanensis gen. nov., sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 60, 196199. [View Article] [PubMed]
    [Google Scholar]
  26. Yoon J.-H., Lee S.-Y., Jung Y.-T., Lee J.-S., Lee K.-C. ( 2013a ). Litorisediminicola beolgyonensis gen. nov., sp. nov., isolated from a coastal sediment. . Int J Syst Evol Microbiol 63, 20252031. [View Article] [PubMed]
    [Google Scholar]
  27. Yoon J.-H., Park S., Jung Y.-T. ( 2013b ). Aestuariihabitans beolgyonensis gen. nov., sp. nov., a novel alphaproteobacterium isolated from tidal flat sediment. . Antonie van Leeuwenhoek 104, 217224. [View Article] [PubMed]
    [Google Scholar]
  28. Zhao B., Wang H., Li R., Mao X. ( 2010 ). Thalassospira xianhensis sp. nov., a polycyclic aromatic hydrocarbon-degrading marine bacterium. . Int J Syst Evol Microbiol 60, 11251129. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.060772-0
Loading
/content/journal/ijsem/10.1099/ijs.0.060772-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed