1887

Abstract

A mesophilic, obligately anaerobic, lactate-, alcohol-, carbohydrate- and amino-acid- degrading bacterium, designated strain 7WAY-8-7, was isolated from an upflow anaerobic sludge blanket reactor treating high-strength organic wastewater from isomerized sugar production processes. Cells of strain 7WAY-8-7 were motile, curved rods (0.7–1.0×5.0–8.0 µm). Spore formation was not observed. The strain grew optimally at 37 °C (range for growth was 25–40 °C) and pH 7.0 (pH 6.0–7.5), and could grow fermentatively on yeast extract, glucose, ribose, xylose, malate, tryptone, pyruvate, fumarate, Casamino acids, serine and cysteine. The main end-products of glucose fermentation were acetate and hydrogen. In co-culture with the hydrogenotrophic methanogen DSM 864, strain 7WAY-8-7 could utilize lactate, glycerol, ethanol, 1-propanol, 1-butanol, -glutamate, alanine, leucine, isoleucine, valine, histidine, asparagine, glutamine, arginine, lysine, threonine, 2-oxoglutarate, aspartate and methionine. A Stickland reaction was not observed with some pairs of amino acids. Yeast extract was required for growth. Nitrate, sulfate, thiosulfate, elemental sulfur, sulfite and Fe (III) were not used as terminal electron acceptors. The G+C content of the genomic DNA was 61.4 mol%. 16S rRNA gene sequence analysis revealed that the isolate belongs to the uncultured environmental clone clade (called ‘PD-UASB-13’ in the Greengenes database) in the bacterial phylum , showing less than 90 % sequence similarity with closely related described species such as and (89.7 % and 88.7 %, respectively). The major cellular fatty acids were iso-C, iso-C, anteiso-C, C, C, C and C. A novel genus and species, gen. nov., sp. nov. is proposed to accommodate strain 7WAY-8-7 ( = JCM 17151 = DSM 24196 = CGMCC 1.5159).

Funding
This study was supported by the:
  • Taishan Scholar Program of Shandong Province
  • Chinese Academy of Sciences (Award KG2D-EW-304-1)
  • 863 Project (Award 2011AA060905)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.060681-0
2014-06-01
2021-07-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/6/2137.html?itemId=/content/journal/ijsem/10.1099/ijs.0.060681-0&mimeType=html&fmt=ahah

References

  1. Allison M. J., Mayberry W. R., McSweeny C. S., Stahl D. A. ( 1992 ). Synergistes jonesii, gen. nov., sp. nov.: a rumen bacterium that degrades toxic pyridinediols. . Syst Appl Microbiol 15, 522529. [CrossRef]
    [Google Scholar]
  2. Baena S., Fardeau M. L., Labat M., Ollivier B., Thomas P., Garcia J. L., Patel B. K. ( 1998 ). Aminobacterium colombiense gen. nov. sp. nov., an amino acid-degrading anaerobe isolated from anaerobic sludge. . Anaerobe 4, 241250. [View Article] [PubMed]
    [Google Scholar]
  3. Baena S., Fardeau M. L., Ollivier B., Labat M., Thomas P., Garcia J. L., Patel B. K. C. ( 1999 ). Aminomonas paucivorans gen. nov., sp. nov., a mesophilic, anaerobic, amino-acid-utilizing bacterium.. Int J Syst Bacteriol 49, 975982. [View Article] [PubMed]
    [Google Scholar]
  4. Baena S., Fardeau M. L., Labat M., Ollivier B., Garcia J. L., Patel B. K. C. ( 2000 ). Aminobacterium mobile sp. nov., a new anaerobic amino-acid-degrading bacterium. . Int J Syst Evol Microbiol 50, 259264. [View Article] [PubMed]
    [Google Scholar]
  5. Caporaso J. G., Bittinger K., Bushman F. D., DeSantis T. Z., Andersen G. L., Knight R. ( 2010 ). PyNAST: a flexible tool for aligning sequences to a template alignment. . Bioinformatics 26, 266267. [View Article] [PubMed]
    [Google Scholar]
  6. Chouari R., Le Paslier D., Dauga C., Daegelen P., Weissenbach J., Sghir A. ( 2005 ). Novel major bacterial candidate division within a municipal anaerobic sludge digester. . Appl Environ Microbiol 71, 21452153. [View Article] [PubMed]
    [Google Scholar]
  7. Dahle H., Birkeland N. K. ( 2006 ). Thermovirga lienii gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, amino-acid-degrading bacterium isolated from a North Sea oil well. . Int J Syst Evol Microbiol 56, 15391545. [View Article] [PubMed]
    [Google Scholar]
  8. Davis C. K., Webb R. I., Sly L. I., Denman S. E., McSweeney C. S. ( 2012 ). Isolation and survey of novel fluoroacetate-degrading bacteria belonging to the phylum Synergistetes . . FEMS Microbiol Ecol 80, 671684. [View Article] [PubMed]
    [Google Scholar]
  9. Delbès C., Moletta R., Godon J. ( 2001 ). Bacterial and archaeal 16S rDNA and 16S rRNA dynamics during an acetate crisis in an anaerobic digestor ecosystem. . FEMS Microbiol Ecol 35, 1926. [View Article] [PubMed]
    [Google Scholar]
  10. Díaz C., Baena S., Fardeau M. L., Patel B. K. ( 2007 ). Aminiphilus circumscriptus gen. nov., sp. nov., an anaerobic amino-acid-degrading bacterium from an upflow anaerobic sludge reactor. . Int J Syst Evol Microbiol 57, 19141918. [View Article] [PubMed]
    [Google Scholar]
  11. Doetsch R. N. ( 1981 ). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 2133. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  12. Downes J., Vartoukian S. R., Dewhirst F. E., Izard J., Chen T., Yu W.-H., Sutcliffe I. C., Wade W. G. ( 2009 ). Pyramidobacter piscolens gen. nov., sp. nov., a member of the phylum ‘Synergistetes’ isolated from the human oral cavity. . Int J Syst Evol Microbiol 59, 972980. [View Article] [PubMed]
    [Google Scholar]
  13. Ganesan A., Chaussonnerie S., Tarrade A., Dauga C., Bouchez T., Pelletier E., Le Paslier D., Sghir A. ( 2008 ). Cloacibacillus evryensis gen. nov., sp. nov., a novel asaccharolytic, mesophilic, amino-acid-degrading bacterium within the phylum ‘Synergistetes’, isolated from an anaerobic sludge digester. . Int J Syst Evol Microbiol 58, 20032012. [View Article] [PubMed]
    [Google Scholar]
  14. Godon J. J., Zumstein E., Dabert P., Habouzit F., Moletta R. ( 1997 ). Molecular microbial diversity of an anaerobic digestor as determined by small-subunit rDNA sequence analysis. . Appl Environ Microbiol 63, 28022813.[PubMed]
    [Google Scholar]
  15. Godon J. J., Morinière J., Moletta M., Gaillac M., Bru V., Delgènes J. P. ( 2005 ). Rarity associated with specific ecological niches in the bacterial world: the ‘Synergistes’ example. . Environ Microbiol 7, 213224. [View Article] [PubMed]
    [Google Scholar]
  16. Hanada S., Takaichi S., Matsuura K., Nakamura K. ( 2002 ). Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. . Int J Syst Evol Microbiol 52, 187193.[PubMed] [CrossRef]
    [Google Scholar]
  17. Hiraishi A. ( 1992 ). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. . Lett Appl Microbiol 15, 210213. [View Article] [PubMed]
    [Google Scholar]
  18. Honda T., Fujita T., Tonouchi A. ( 2013 ). Aminivibrio pyruvatiphilus gen. nov., sp. nov., an anaerobic, amino-acid-degrading bacterium from soil of a Japanese rice field. . Int J Syst Evol Microbiol 63, 36793686. [View Article] [PubMed]
    [Google Scholar]
  19. Horz H. P., Citron D. M., Warren Y. A., Goldstein E. J., Conrads G. ( 2006 ). Synergistes group organisms of human origin. . J Clin Microbiol 44, 29142920. [View Article] [PubMed]
    [Google Scholar]
  20. Hugenholtz P., Hooper S. D., Kyrpides N. C. ( 2009 ). Focus: Synergistetes . . Environ Microbiol 11, 13271329. [View Article] [PubMed]
    [Google Scholar]
  21. Ito T., Yoshiguchi K., Ariesyady H. D., Okabe S. ( 2011 ). Identification of a novel acetate-utilizing bacterium belonging to Synergistes group 4 in anaerobic digester sludge. . ISME J 5, 18441856. [View Article] [PubMed]
    [Google Scholar]
  22. Jumas-Bilak E., Carlier J. P., Jean-Pierre H., Citron D., Bernard K., Damay A., Gay B., Teyssier C., Campos J., Marchandin H. ( 2007 ). Jonquetella anthropi gen. nov., sp. nov., the first member of the candidate phylum ‘Synergistetes’ isolated from man. . Int J Syst Evol Microbiol 57, 27432748. [View Article] [PubMed]
    [Google Scholar]
  23. Jumas-Bilak E., Roudière L., Marchandin H. ( 2009 ). Description of ‘Synergistetes’ phyl. nov. and emended description of the phylum ‘Deferribacteres’ and of the family Syntrophomonadaceae, phylum ‘Firmicutes’. . Int J Syst Evol Microbiol 59, 10281035. [View Article] [PubMed]
    [Google Scholar]
  24. Kamagata Y., Mikami E. ( 1991 ). Isolation and characterization of a novel thermophilic Methanosaeta strain. . Int J Syst Bacteriol 41, 191196. [View Article]
    [Google Scholar]
  25. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  26. McDonald D., Price M. N., Goodrich J., Nawrocki E. P., DeSantis T. Z., Probst A., Andersen G. L., Knight R., Hugenholtz P. ( 2012 ). An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea. . ISME J 6, 610618. [View Article] [PubMed]
    [Google Scholar]
  27. Menes R. J., Muxí L. ( 2002 ). Anaerobaculum mobile sp. nov., a novel anaerobic, moderately thermophilic, peptide-fermenting bacterium that uses crotonate as an electron acceptor, and emended description of the genus Anaerobaculum . . Int J Syst Evol Microbiol 52, 157164.[PubMed]
    [Google Scholar]
  28. Narihiro T., Terada T., Kikuchi K., Iguchi A., Ikeda M., Yamauchi T., Shiraishi K., Kamagata Y., Nakamura K., Sekiguchi Y. ( 2009 ). Comparative analysis of bacterial and archaeal communities in methanogenic sludge granules from upflow anaerobic sludge blanket reactors treating various food-processing, high-strength organic wastewaters. . Microbes Environ 24, 8896. [View Article] [PubMed]
    [Google Scholar]
  29. Rees G. N., Patel B. K. C., Grassia G. S., Sheehy A. J. ( 1997 ). Anaerobaculum thermoterrenum gen. nov., sp. nov., a novel, thermophilic bacterium which ferments citrate. . Int J Syst Bacteriol 47, 150154. [View Article] [PubMed]
    [Google Scholar]
  30. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  31. Salminen E., Rintala J. ( 2002 ). Anaerobic digestion of organic solid poultry slaughterhouse waste–a review. . Bioresour Technol 83, 1326. [View Article] [PubMed]
    [Google Scholar]
  32. Schink B., Stams A. J. ( 2006 ). Syntrophism among prokaryotes. . In The Prokaryotes, , 3rd edn., vol 2, pp. 309335. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. . New York:: Springer;. [View Article]
    [Google Scholar]
  33. Sekiguchi Y., Kamagata Y., Nakamura K., Ohashi A., Harada H. ( 2000 ). Syntrophothermus lipocalidus gen. nov., sp. nov., a novel thermophilic, syntrophic, fatty-acid-oxidizing anaerobe which utilizes isobutyrate. . Int J Syst Evol Microbiol 50, 771779. [View Article] [PubMed]
    [Google Scholar]
  34. Sekiguchi Y., Yamada T., Hanada S., Ohashi A., Harada H., Kamagata Y. ( 2003 ). Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level. . Int J Syst Evol Microbiol 53, 18431851. [View Article] [PubMed]
    [Google Scholar]
  35. Shintani T., Liu W. T., Hanada S., Kamagata Y., Miyaoka S., Suzuki T., Nakamura K. ( 2000 ). Micropruina glycogenica gen. nov., sp. nov., a new Gram-positive glycogen-accumulating bacterium isolated from activated sludge. . Int J Syst Evol Microbiol 50, 201207. [View Article] [PubMed]
    [Google Scholar]
  36. Stamatakis A. ( 2006 ). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22, 26882690. [View Article] [PubMed]
    [Google Scholar]
  37. Surkov A. V., Dubinina G. A., Lysenko A. M., Glöckner F. O., Kuever J. ( 2001 ). Dethiosulfovibrio russensis sp. nov., Dethosulfovibrio marinus sp. nov. and Dethosulfovibrio acidaminovorans sp. nov., novel anaerobic, thiosulfate- and sulfur-reducing bacteria isolated from ‘Thiodendron’ sulfur mats in different saline environments. . Int J Syst Evol Microbiol 51, 327337.[PubMed]
    [Google Scholar]
  38. Swofford, D. L. (2003). paup*: phylogenetic analysis using parsimony (*and other methods), version 4., Sunderland, MA: Sinauer Associates.
  39. Tindall B. J. ( 1990 ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  40. Vartoukian S. R., Palmer R. M., Wade W. G. ( 2007 ). The division “Synergistes” . . Anaerobe 13, 99106. [View Article] [PubMed]
    [Google Scholar]
  41. Vartoukian S. R., Downes J., Palmer R. M., Wade W. G. ( 2013 ). Fretibacterium fastidiosum gen. nov., sp. nov., isolated from the human oral cavity. . Int J Syst Evol Microbiol 63, 458463. [View Article] [PubMed]
    [Google Scholar]
  42. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703.[PubMed]
    [Google Scholar]
  43. Zavarzina D. G., Zhilina T. N., Tourova T. P., Kuznetsov B. B., Kostrikina N. A., Bonch-Osmolovskaya E. A. ( 2000 ). Thermanaerovibrio velox sp. nov., a new anaerobic, thermophilic, organotrophic bacterium that reduces elemental sulfur, and emended description of the genus Thermanaerovibrio . . Int J Syst Evol Microbiol 50, 12871295. [View Article] [PubMed]
    [Google Scholar]
  44. Zhang T., Ke S. Z., Liu Y., Fang H. P. ( 2005 ). Microbial characteristics of a methanogenic phenol-degrading sludge. . Water Sci Technol 52, 7378.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.060681-0
Loading
/content/journal/ijsem/10.1099/ijs.0.060681-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error