1887

Abstract

A Gram-staining-negative, non-motile, non-pigmented and rod-shaped bacterial strain, designated IMCC12053, was isolated from coastal surface seawater of the Yellow Sea, Korea. Optimal growth of strain IMCC12053 was observed at 30 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl. 16S rRNA gene sequence comparisons showed that strain IMCC12053 was most closely related to L-6 (97.5 % similarity) and H 14 (96.0 %). Strain IMCC12053 formed a robust phylogenetic clade with members of the genus . The DNA–DNA relatedness value between IMCC12053 and was far lower than 70 % (35.7–42.5 %), which indicated that strain IMCC12053 is a novel genomic species of the genus . The major respiratory isoprenoid quinone was ubiquinone-10 (Q-10) and major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and aminolipids. The DNA G+C content was 61.0 mol%. On the basis of genotypic and phenotypic data collected in this study, it is proposed that strain IMCC12053 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMCC12053 ( = KACC 17482 = NBRC 109702).

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2014-04-01
2019-10-18
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. ( 1997;). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef][PubMed]
    [Google Scholar]
  2. Cho J.-C., Giovannoni S. J.. ( 2004;). Cultivation and growth characteristics of a diverse group of oligotrophic marine Gammaproteobacteria. . Appl Environ Microbiol 70:, 432–440. [CrossRef][PubMed]
    [Google Scholar]
  3. Choo Y.-J., Lee K., Song J., Cho J.-C.. ( 2007;). Puniceicoccus vermicola gen. nov., sp. nov., a novel marine bacterium, and description of Puniceicoccaceae fam. nov., Puniceicoccales ord. nov., Opitutaceae fam. nov., Opitutales ord. nov. and Opitutae classis nov. in the phylum ‘Verrucomicrobia’. . Int J Syst Evol Microbiol 57:, 532–537. [CrossRef][PubMed]
    [Google Scholar]
  4. Collins M.. ( 1985;). Analysis of isoprenoid quinones. . Methods Microbiol 18:, 329–366.
    [Google Scholar]
  5. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  6. Fitch W. M.. ( 1971;). Toward defining the course of evolution: Minimum change for a specific tree topology. . Syst Biol 20:, 406–416. [CrossRef]
    [Google Scholar]
  7. Giovannoni S. J., Rappé M.. S. ( 2000;). Evolution, diversity and molecular ecology of marine prokaryotes. . In Microbial Ecology of the Oceans, pp. 47–84. Edited by Kirchman D. L... New York:: Wiley;.
    [Google Scholar]
  8. Ivanova E. P., Webb H., Christen R., Zhukova N. V., Kurilenko V. V., Kalinovskaya N. I., Crawford R. J.. ( 2010;). Celeribacter neptunius gen. nov., sp. nov., a new member of the class Alphaproteobacteria. . Int J Syst Evol Microbiol 60:, 1620–1625. [CrossRef][PubMed]
    [Google Scholar]
  9. Jorgensen J. H., Turnidge J. D., Washington J. A.. ( 1999;). Antibacterial susceptibility tests: dilution and disk diffusion methods. . In Manual of Clinical Microbiology, pp. 1526–1543. Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  10. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–32. Edited by Munro H. N... New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  11. Kang I., Jang H., Oh H.-M., Cho J.-C.. ( 2012;). Complete genome sequence of Celeribacter bacteriophage P12053L. . J Virol 86:, 8339–8340. [CrossRef][PubMed]
    [Google Scholar]
  12. Kim O. S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  13. Lee S.-Y., Park S., Oh T. K., Yoon J. H.. ( 2012;). Celeribacter baekdonensis sp. nov., isolated from seawater, and emended description of the genus Celeribacter Ivanova et al. 2010. . Int J Syst Evol Microbiol 62:, 1359–1364. [CrossRef][PubMed]
    [Google Scholar]
  14. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S.. & other authors ( 2004;). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef][PubMed]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by highperformance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  16. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  17. Pruesse E., Peplies J., Glöckner F. O.. ( 2012;). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28:, 1823–1829. [CrossRef][PubMed]
    [Google Scholar]
  18. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  21. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R.. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
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