1887

Abstract

Two halophilic archaeal strains, TRM20010 and TRM20345, were isolated from saline soil of the Lop Nur region in Xinjiang, north-west China. Cells from the two strains were pleomorphic rods, stained Gram-negative and produced red-pigmented colonies. Strains TRM20010 and TRM20345 were able to grow at 30–62 °C (optimum 37 °C), 0.9–5.1 M NaCl (optimum 2.6 and 3.4 M, respectively) and pH 6.0–10.0 (optimum pH 7.0−7.5) and neither strain required Mg for growth. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), two glycolipids chromatographically identical to galactosyl mannosyl glucosyl diether (TGD-1) and disulfated mannosyl glucosyl diether (S-DGD). Phylogenetic analysis based on 16S rRNA and genes revealed that strains TRM20010 and TRM20345 clustered together and formed a distinct clade separated from the related genera , , , and . The DNA G+C contents of strains TRM20010 and TRM20345 were 63.9 and 63.8 mol%, respectively. The DNA–DNA hybridization value between strain TRM20010 and strain TRM20345 was 42.8 %. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strains TRM20010 and TRM20345 represent two novel species in a new genus within the family , for which the names gen. nov., sp. nov. (type strain TRM20010 = CCTCC AB2013112 = NRRL B-59996) and sp. nov. (type strain TRM20345 = CCTCC AB2013113 = NRRL B-59997) are proposed.

Funding
This study was supported by the:
  • , National High-tech R&D Program of China , (Award 2012AA021705)
  • , Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin , (Award BRZD1206)
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2015-02-01
2021-02-27
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