1887

Abstract

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, AH-M5, which was isolated from a tidal flat sediment at Aphae Island in South Korea, was characterized taxonomically. Strain AH-M5 grew optimally at 25 °C, at pH 7.0–8.0 and in presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain AH-M5 clustered coherently with the type strains of and , showing 93.4–94.3 % sequence similarity. The novel strain exhibited 16S rRNA gene sequence similarity values of less than 93.4 % to the type strains of other recognized species. Strain AH-M5 contained MK-6 as the predominant menaquinone and iso-C G, iso-C, iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6) as the major fatty acids. The polar lipid profile of strain AH-M5 containing phosphatidylethanolamine and one unidentified lipid as major components was differentiated from those of the type strains of and . The DNA G+C content of strain AH-M5 was 34.8 mol%. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrated that strain AH-M5 is distinguished from and . On the basis of the data presented, strain AH-M5 is considered to represent a novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is AH-M5 ( = KCTC 32578 = CECT 8487).

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2014-06-01
2019-10-16
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References

  1. Barrow G. I., Feltham R. K. A.. (editors) ( 1993;). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F.. ( 2011;). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 106–111. Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  3. Bernardet J.-F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  4. Bowman J. P.. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  5. Bruns A., Rohde M., Berthe-Corti L.. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef][PubMed]
    [Google Scholar]
  6. Jeong S. H., Park M. S., Jin H. M., Lee K., Park W., Jeon C. O.. ( 2013;). Aestuariibaculum suncheonense gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a tidal flat and emended descriptions of the genera Gaetbulibacter and Tamlana. . Int J Syst Evol Microbiol 63:, 332–338. [CrossRef][PubMed]
    [Google Scholar]
  7. Jung S.-Y., Kang S.-J., Lee M.-H., Lee S.-Y., Oh T.-K., Yoon J.-H.. ( 2005;). Gaetbulibacter saemankumensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat sediment in Korea. . Int J Syst Evol Microbiol 55:, 1845–1849. [CrossRef][PubMed]
    [Google Scholar]
  8. Jung Y.-T., Lee J.-S., Yoon J.-H.. ( 2013;). Hwangdonia seohaensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 63:, 3186–3191. [CrossRef][PubMed]
    [Google Scholar]
  9. Kim J.-H., Kim K.-Y., Hahm Y.-T., Kim B.-S., Chun J., Cha C.-J.. ( 2008;). Actibacter sediminis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment. . Int J Syst Evol Microbiol 58:, 139–143. [CrossRef][PubMed]
    [Google Scholar]
  10. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  11. Kwon K. K., Lee H.-S., Jung H.-B., Kang J.-H., Kim S.-J.. ( 2006;). Yeosuana aromativorans gen. nov., sp. nov., a mesophilic marine bacterium belonging to the family Flavobacteriaceae, isolated from estuarine sediment of the South Sea, Korea. . Int J Syst Evol Microbiol 56:, 727–732. [CrossRef][PubMed]
    [Google Scholar]
  12. Lányi B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  13. Lee S.-Y., Lee M.-H., Oh T.-K., Yoon J.-H.. ( 2012;). Lutibacter aestuarii sp. nov., isolated from a tidal flat sediment, and emended description of the genus Lutibacter Choi and Cho 2006. . Int J Syst Evol Microbiol 62:, 420–424. [CrossRef][PubMed]
    [Google Scholar]
  14. Leifson E.. ( 1963;). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  15. Li Y., Bai S., Yang C., Lai Q., Zhang H., Chen Z., Wei J., Zheng W., Tian Y., Zheng T.. ( 2013;). Mangrovimonas yunxiaonensis gen. nov., sp. nov., isolated from mangrove sediment. . Int J Syst Evol Microbiol 63:, 2043–2048. [CrossRef][PubMed]
    [Google Scholar]
  16. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  18. Park S. C., Choe H. N., Hwang Y. M., Baik K. S., Kim S. N., Lee Y. S., Jung J. S., Seong C. N.. ( 2013;). Marinivirga aestuarii gen. nov., sp. nov., a member of the family Flavobacteriaceae, isolated from marine environments, and emended descriptions of the genera Hyunsoonleella, Jejuia and Pontirhabdus and the species Hyunsoonleella jejuensis, Jejuia pallidilutea and Pontirhabdus pectinivorans. . Int J Syst Evol Microbiol 63:, 1524–1531. [CrossRef][PubMed]
    [Google Scholar]
  19. Reichenbach H.. ( 1992;). The order Cytophagales. . In The Prokaryotes, , 2nd edn., vol. 4, pp. 3631–3675. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H... New York:: Springer;. [CrossRef]
    [Google Scholar]
  20. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. , MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
  21. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  22. Wang B., Sun F., Du Y., Liu X., Li G., Lai Q., Luo J., Shao Z.. ( 2010;). Meridianimaribacter flavus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment of the South China Sea. . Int J Syst Evol Microbiol 60:, 121–127. [CrossRef][PubMed]
    [Google Scholar]
  23. Yi H., Chun J.. ( 2011;). Snuella lapsa gen. nov., sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 61:, 2469–2474. [CrossRef][PubMed]
    [Google Scholar]
  24. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H.. ( 1996;). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46:, 502–505. [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Lee S. T., Park Y.-H.. ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef][PubMed]
    [Google Scholar]
  26. Yoon J.-H., Kang K. H., Park Y.-H.. ( 2003;). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 53:, 449–454. [CrossRef][PubMed]
    [Google Scholar]
  27. Yoon J.-H., Kang S.-J., Jung Y.-T., Oh T.-K.. ( 2008;). Aestuariicola saemankumensis gen. nov., sp. nov., a member of the family Flavobacteriaceae, isolated from tidal flat sediment. . Int J Syst Evol Microbiol 58:, 2126–2131. [CrossRef][PubMed]
    [Google Scholar]
  28. Yoon J.-H., Lee S.-Y., Oh T.-K.. ( 2013;). Gaetbulibacter lutimaris sp. nov., isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 63:, 995–1000. [CrossRef][PubMed]
    [Google Scholar]
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