1887

Abstract

A Gram-reaction-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, AY17, was isolated from the Chishui River in Guizhou Province, South-west China. Strain AY17 grew optimally at pH 7.0 and 20 °C. Flexirubin-type pigments were produced. 16S rRNA gene sequence analysis indicated that strain AY17 belonged to the family within the phylum ; the closest phylogenetic relative was PTJT-5 (95.3 % gene sequence similarity). The DNA G+C content was 49.5 mol%. Strain AY17 contained MK-7 as the predominant respiratory quinone and phosphatidylethanolamine as the major polar lipid. The major fatty acids were iso-C, iso-CG and iso-C 3-OH. On the basis of phylogenetic, phenotypic and genetic data, strain AY17 was classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AY17 ( = CGMCC 1.12700 = JCM 19637).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.060269-0
2014-05-01
2020-05-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/5/1795.html?itemId=/content/journal/ijsem/10.1099/ijs.0.060269-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Barrow G. I., Feltham R. K. A. (editors) ( 1993 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  3. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [CrossRef] [PubMed]
    [Google Scholar]
  4. da Costa M. S., Albuquerque L., Nobre M., Wait R. ( 2011a ). The extraction and identification of respiratory lipoquinones of prokaryotes and their use in taxonomy. . Methods Microbiol 38, 197206. [CrossRef]
    [Google Scholar]
  5. da Costa M. S., Albuquerque L., Nobre M., Wait R. ( 2011b ). The identification of polar lipids in prokaryotes. . Methods Microbiol 38, 165181. [CrossRef]
    [Google Scholar]
  6. Fautz E., Reichenbach H. ( 1980 ). A simple test for flexirubin-type pigments. . FEMS Microbiol Lett 8, 8791. [CrossRef]
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  8. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Biol 20, 406416. [CrossRef]
    [Google Scholar]
  9. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598. [CrossRef]
    [Google Scholar]
  10. Kämpfer P., Young C. C., Sridhar K. R., Arun A. B., Lai W. A., Shen F. T., Rekha P. D. ( 2006 ). Transfer of [Flexibacter] sancti, [Flexibacter] filiformis, [Flexibacter] japonensis and [Cytophaga] arvensicola to the genus Chitinophaga and description of Chitinophaga skermanii sp. nov.. Int J Syst Evol Microbiol 56, 22232228. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kishino H., Hasegawa M. ( 1989 ). Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea. . J Mol Evol 29, 170179. [CrossRef] [PubMed]
    [Google Scholar]
  14. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 125175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  15. Lee H. G., An D. S., Im W. T., Liu Q. M., Na J. R., Cho D. H., Jin C. W., Lee S. T., Yang D. C. ( 2007 ). Chitinophaga ginsengisegetis sp. nov. and Chitinophaga ginsengisoli sp. nov., isolated from soil of a ginseng field in South Korea. . Int J Syst Evol Microbiol 57, 13961401. [CrossRef] [PubMed]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [CrossRef]
    [Google Scholar]
  18. Murray R., Doetsch R. N., Robinow C. F. ( 1994 ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 2141. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  19. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  20. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  21. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  23. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [CrossRef] [PubMed]
    [Google Scholar]
  24. Zhang L., Wang Y., Wei L., Wang Y., Shen X., Li S. ( 2013 ). Taibaiella smilacinae gen. nov., sp. nov., an endophytic member of the family Chitinophagaceae isolated from the stem of Smilacina japonica, and emended description of Flavihumibacter petaseus . . Int J Syst Evol Microbiol 63, 37693776. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.060269-0
Loading
/content/journal/ijsem/10.1099/ijs.0.060269-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error