1887

International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue 5

sp. nov., isolated from freshwater Free

Abstract

A Gram-reaction-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, AY17, was isolated from the Chishui River in Guizhou Province, South-west China. Strain AY17 grew optimally at pH 7.0 and 20 °C. Flexirubin-type pigments were produced. 16S rRNA gene sequence analysis indicated that strain AY17 belonged to the family within the phylum ; the closest phylogenetic relative was PTJT-5 (95.3 % gene sequence similarity). The DNA G+C content was 49.5 mol%. Strain AY17 contained MK-7 as the predominant respiratory quinone and phosphatidylethanolamine as the major polar lipid. The major fatty acids were iso-C, iso-CG and iso-C 3-OH. On the basis of phylogenetic, phenotypic and genetic data, strain AY17 was classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AY17 ( = CGMCC 1.12700 = JCM 19637).

  • Published Online:
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.060269-0
2014-05-01
2019-10-13

  • From This Site

    /content/journal/ijsem/10.1099/ijs.0.060269-0
    dcterms_title,dcterms_subject,pub_keyword
    10
    5
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/5/1795.html?itemId=/content/journal/ijsem/10.1099/ijs.0.060269-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403––410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Barrow G. I. , Feltham R. K. A. . (editors) ( 1993; ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  3. Bernardet J.-F. , Nakagawa Y. , Holmes B. . Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049––1070. [CrossRef] [PubMed]
    [Google Scholar]
  4. da Costa M. S. , Albuquerque L. , Nobre M. , Wait R. . ( 2011a; ). The extraction and identification of respiratory lipoquinones of prokaryotes and their use in taxonomy. . Methods Microbiol 38:, 197––206. [CrossRef]
    [Google Scholar]
  5. da Costa M. S. , Albuquerque L. , Nobre M. , Wait R. . ( 2011b; ). The identification of polar lipids in prokaryotes. . Methods Microbiol 38:, 165––181. [CrossRef]
    [Google Scholar]
  6. Fautz E. , Reichenbach H. . ( 1980; ). A simple test for flexirubin-type pigments. . FEMS Microbiol Lett 8:, 87––91. [CrossRef]
    [Google Scholar]
  7. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783––791. [CrossRef]
    [Google Scholar]
  8. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Biol 20:, 406––416. [CrossRef]
    [Google Scholar]
  9. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95––98. [CrossRef]
    [Google Scholar]
  10. Kämpfer P. , Young C. C. , Sridhar K. R. , Arun A. B. , Lai W. A. , Shen F. T. , Rekha P. D. . ( 2006; ). Transfer of [Flexibacter] sancti, [Flexibacter] filiformis, [Flexibacter] japonensis and [Cytophaga] arvensicola to the genus Chitinophaga and description of Chitinophaga skermanii sp. nov.. Int J Syst Evol Microbiol 56:, 2223––2228. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716––721. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111––120. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kishino H. , Hasegawa M. . ( 1989; ). Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea. . J Mol Evol 29:, 170––179. [CrossRef] [PubMed]
    [Google Scholar]
  14. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 125––175. Edited by Stackebrandt E. , Goodfellow M. . . Chichester:: Wiley;.
     [Google Scholar]
  15. Lee H. G. , An D. S. , Im W. T. , Liu Q. M. , Na J. R. , Cho D. H. , Jin C. W. , Lee S. T. , Yang D. C. . ( 2007; ). Chitinophaga ginsengisegetis sp. nov. and Chitinophaga ginsengisoli sp. nov., isolated from soil of a ginseng field in South Korea. . Int J Syst Evol Microbiol 57:, 1396––1401. [CrossRef] [PubMed]
    [Google Scholar]
  16. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159––167. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233––241. [CrossRef]
    [Google Scholar]
  18. Murray R. , Doetsch R. N. , Robinow C. F. . ( 1994; ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21––41. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  19. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406––425.[PubMed]
    [Google Scholar]
  20. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  21. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607––654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  22. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731––2739. [CrossRef] [PubMed]
    [Google Scholar]
  23. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673––4680. [CrossRef] [PubMed]
    [Google Scholar]
  24. Zhang L. , Wang Y. , Wei L. , Wang Y. , Shen X. , Li S. . ( 2013; ). Taibaiella smilacinae gen. nov., sp. nov., an endophytic member of the family Chitinophagaceae isolated from the stem of Smilacina japonica, and emended description of Flavihumibacter petaseus . . Int J Syst Evol Microbiol 63:, 3769––3776. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.060269-0
Loading
Taibaiellachishuiensis sp. nov., isolated from freshwater
Int J Syst Evol Microbiol 64, 1795 (2014); https://doi.org/10.1099/ijs.0.060269-0
/content/journal/ijsem/10.1099/ijs.0.060269-0
/content/journal/ijsem/10.1099/ijs.0.060269-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error