1887

Abstract

A novel, red–pink-pigmented strain, designated R2-4, was isolated from a till sample near Ny-Alesund, Svalbard Archipelago, Norway. Cells were aerobic, Gram-stain-negative and rod-shaped. Growth occurred at 4–30 °C (optimum, 20–22 °C), at pH 6.0–9.0 (optimum, pH 7.0) and with 0–1 % NaCl added to R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R2-4 belonged to the genus . 16S rRNA gene sequence similarity between strain R2-4 and the type strains of related species of the genus ranged from 94.51 to 96.05 %. Strain R2-4 contained iso-C, anteiso-C, summed feature 3 (Cω6 and/or Cω7), summed feature 4 (C anteiso B and/or iso I) and Cω5 as the major cellular fatty acids, MK-7 as the major respiratory quinone, and phosphatidylethanolamine, unknown aminophospholipids, unknown aminolipids and unknown lipids as the main polar lipids. The polyamine was -homospermidine. The DNA G+C content of strain R2-4 was 61.6 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain R2-4 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R2-4 ( = CCTCC AB 2012104 = KACC 16881).

Erratum
This article contains a correction applying to the following content:
Erratum to Hymenobacter arcticus sp. nov., isolated from glacial till
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2014-06-01
2019-10-15
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References

  1. Baik K. S., Seong C. N., Moon E. Y., Park Y. D., Yi H., Chun J.. ( 2006;). Hymenobacter rigui sp. nov., isolated from wetland freshwater. . Int J Syst Evol Microbiol 56:, 2189–2192. [CrossRef][PubMed]
    [Google Scholar]
  2. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M.. ( 1966;). Antibiotic susceptibility testing by a standardized single disk method. . Am J Clin Pathol 45:, 493–496.[PubMed]
    [Google Scholar]
  3. Bowman J. P.. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  4. Buczolits S., Denner E. B., Vybiral D., Wieser M., Kämpfer P., Busse H. J.. ( 2002;). Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov.. Int J Syst Evol Microbiol 52:, 445–456.[PubMed]
    [Google Scholar]
  5. Buczolits S., Denner E. B., Kämpfer P., Busse H. J.. ( 2006;). Proposal of Hymenobacter norwichensis sp. nov., classification of ‘Taxeobacter ocellatus’, ‘Taxeobacter gelupurpurascens’ and ‘Taxeobacter chitinovorans’ as Hymenobacter ocellatus sp. nov., Hymenobacter gelipurpurascens sp. nov. and Hymenobacter chitinivorans sp. nov., respectively, and emended description of the genus Hymenobacter Hirsch et al. 1999. . Int J Syst Evol Microbiol 56:, 2071–2078. [CrossRef][PubMed]
    [Google Scholar]
  6. Busse H.-J., Auling G.. ( 1988;). Polyamine pattern as a chemotaxonomic marker within the proteobacteria. . Syst Appl Microbiol 11:, 1–8. [CrossRef]
    [Google Scholar]
  7. Busse H.-J., Bunka S., Hensel A., Lubitz W.. ( 1997;). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. . Int J Syst Bacteriol 47:, 698–708. [CrossRef]
    [Google Scholar]
  8. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef][PubMed]
    [Google Scholar]
  9. Chung A. P., Lopes A., Nobre M. F., Morais P. V.. ( 2010;). Hymenobacter perfusus sp. nov., Hymenobacter flocculans sp. nov. and Hymenobacter metalli sp. nov. three new species isolated from an uranium mine waste water treatment system. . Syst Appl Microbiol 33:, 436–443. [CrossRef][PubMed]
    [Google Scholar]
  10. Collins M. D., Hutson R. A., Grant I. R., Patterson M. F.. ( 2000;). Phylogenetic characterization of a novel radiation-resistant bacterium from irradiated pork: description of Hymenobacter actinosclerus sp. nov.. Int J Syst Evol Microbiol 50:, 731–734. [CrossRef][PubMed]
    [Google Scholar]
  11. Dai J., Wang Y., Zhang L., Tang Y., Luo X., An H., Fang C.. ( 2009;). Hymenobacter tibetensis sp. nov., a UV-resistant bacterium isolated from Qinghai–Tibet plateau. . Syst Appl Microbiol 32:, 543–548. [CrossRef][PubMed]
    [Google Scholar]
  12. Doetsch R. N.. ( 1981;). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 21–33. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  13. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  14. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  15. Hirsch P., Ludwig W., Hethke C., Sittig M., Hoffmann B., Gallikowski C. A.. ( 1998;). Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antartica soils and sandstone: bacteria of the Cytophaga/Flavobacterium/Bacteroides line of phylogenetic descent. . Syst Appl Microbiol 21:, 374–383. [CrossRef][PubMed]
    [Google Scholar]
  16. Hoang V. A., Kim Y. J., Nguyen N. L., Yang D. C.. ( 2013;). Hymenobacter ginsengisoli sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 63:, 661–666. [CrossRef][PubMed]
    [Google Scholar]
  17. Joung Y., Cho S. H., Kim H., Kim S. B., Joh K.. ( 2011;). Hymenobacter yonginensis sp. nov., isolated from a mesotrophic artificial lake. . Int J Syst Evol Microbiol 61:, 1511–1514. [CrossRef][PubMed]
    [Google Scholar]
  18. Kim K. H., Im W. T., Lee S. T.. ( 2008;). Hymenobacter soli sp. nov., isolated from grass soil. . Int J Syst Evol Microbiol 58:, 941–945. [CrossRef][PubMed]
    [Google Scholar]
  19. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  20. Klassen J. L., Foght J. M.. ( 2011;). Characterization of Hymenobacter isolates from Victoria Upper Glacier, Antarctica reveals five new species and substantial non-vertical evolution within this genus. . Extremophiles 15:, 45–57. [CrossRef][PubMed]
    [Google Scholar]
  21. Kovacs N.. ( 1956;). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178:, 703. [CrossRef][PubMed]
    [Google Scholar]
  22. Mesbah M., Whitman W. B.. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479:, 297–306. [CrossRef][PubMed]
    [Google Scholar]
  23. Reddy G. S., Garcia-Pichel F.. ( 2013;). Description of Hymenobacter arizonensis sp. nov. from the southwestern arid lands of the United States of America. . Antonie van Leeuwenhoek 103:, 321–330. [CrossRef][PubMed]
    [Google Scholar]
  24. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  25. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc. .
    [Google Scholar]
  26. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  27. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  28. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  29. Tindall B. J.. ( 1990;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  30. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  31. Xie C. H., Yokota A.. ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49:, 345–349. [CrossRef][PubMed]
    [Google Scholar]
  32. Xu J. L., Liu Q. M., Yu H. S., Jin F. X., Lee S. T., Im W. T.. ( 2009;). Hymenobacter daecheongensis sp. nov., isolated from stream sediment. . Int J Syst Evol Microbiol 59:, 1183–1187. [CrossRef][PubMed]
    [Google Scholar]
  33. Zhang Q., Liu C., Tang Y., Zhou G., Shen P., Fang C., Yokota A.. ( 2007;). Hymenobacter xinjiangensis sp. nov., a radiation-resistant bacterium isolated from the desert of Xinjiang, China. . Int J Syst Evol Microbiol 57:, 1752–1756. [CrossRef][PubMed]
    [Google Scholar]
  34. Zhang G., Niu F., Busse H. J., Ma X., Liu W., Dong M., Feng H., An L., Cheng G.. ( 2008;). Hymenobacter psychrotolerans sp. nov., isolated from the Qinghai–Tibet Plateau permafrost region. . Int J Syst Evol Microbiol 58:, 1215–1220. [CrossRef][PubMed]
    [Google Scholar]
  35. Zhang L., Dai J., Tang Y., Luo X., Wang Y., An H., Fang C., Zhang C.. ( 2009;). Hymenobacter deserti sp. nov., isolated from the desert of Xinjiang, China. . Int J Syst Evol Microbiol 59:, 77–82. [CrossRef][PubMed]
    [Google Scholar]
  36. Zhang D. C., Busse H. J., Liu H. C., Zhou Y. G., Schinner F., Margesin R.. ( 2011;). Hymenobacter psychrophilus sp. nov., a psychrophilic bacterium isolated from soil. . Int J Syst Evol Microbiol 61:, 859–863. [CrossRef][PubMed]
    [Google Scholar]
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