sp. nov., an actinomycete isolated from soil Free

Abstract

A Gram-staining-positive, aerobic, non-spore-forming, irregular rod-shaped actinobacterium, designated YIM C00895, was isolated from a soil sample collected from Jiuxiang Scenic Region, Yunnan province, south-west China. The strain was able to grow at 10–28 °C, pH 6.0–10.0 and 0–11 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA (95.3 %) gene sequences revealed the highest similarity to AHU1821. The whole-cell hydrolysates of strain YIM C00895 contained -diaminopimelic acid, arabinose and galactose, indicating chemotype IV. The muramic acids in the peptidoglycan were glycolated. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified glycolipid. MK-9(H) was the predominant menaquinone. The major fatty acids were C, C, C and Cω9 and tuberculostearic acid (10-methyl C) was present in relatively small amounts (4.5 %). TLC analysis of its cellular mycolic acids showed a similar profile to DSM 45403. The DNA G+C content of the strain was 67.5 mol%. The results of physiological and biochemical tests allowed strain YIM C00895 to be differentiated phenotypically from DSM 45403. On the basis of evidence from this polyphasic study, the novel species sp. nov. is proposed. The type strain of is YIM C00895 ( = DSM 45788 = JCM 18542).

Funding
This study was supported by the:
  • National New Drug Research and Development Project (Award 2010ZX09401-403)
  • China Tobacco Yunnan Industrial Co. Ltd (Award 2012FL02 and 2012JC07)
  • Chinese Academy of Sciences
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2014-07-01
2024-03-29
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