1887

Abstract

A Gram-staining-positive, aerobic, non-spore-forming, irregular rod-shaped actinobacterium, designated YIM C00895, was isolated from a soil sample collected from Jiuxiang Scenic Region, Yunnan province, south-west China. The strain was able to grow at 10–28 °C, pH 6.0–10.0 and 0–11 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA (95.3 %) gene sequences revealed the highest similarity to AHU1821. The whole-cell hydrolysates of strain YIM C00895 contained -diaminopimelic acid, arabinose and galactose, indicating chemotype IV. The muramic acids in the peptidoglycan were glycolated. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified glycolipid. MK-9(H) was the predominant menaquinone. The major fatty acids were C, C, C and Cω9 and tuberculostearic acid (10-methyl C) was present in relatively small amounts (4.5 %). TLC analysis of its cellular mycolic acids showed a similar profile to DSM 45403. The DNA G+C content of the strain was 67.5 mol%. The results of physiological and biochemical tests allowed strain YIM C00895 to be differentiated phenotypically from DSM 45403. On the basis of evidence from this polyphasic study, the novel species sp. nov. is proposed. The type strain of is YIM C00895 ( = DSM 45788 = JCM 18542).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.060202-0
2014-07-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/7/2319.html?itemId=/content/journal/ijsem/10.1099/ijs.0.060202-0&mimeType=html&fmt=ahah

References

  1. Adachi K., Katsuta A., Matsuda S., Peng X., Misawa N., Shizuri Y., Kroppenstedt R. M., Yokota A., Kasai H.. ( 2007;). Smaragdicoccus niigatensis gen. nov., sp. nov., a novel member of the suborder Corynebacterineae. . Int J Syst Evol Microbiol 57:, 297–301. [CrossRef][PubMed]
    [Google Scholar]
  2. Butler W. R., Floyd M. M., Brown J. M., Toney S. R., Daneshvar M. I., Cooksey R. C., Carr J., Steigerwalt A. G., Charles N.. ( 2005;). Novel mycolic acid-containing bacteria in the family Segniliparaceae fam. nov., including the genus Segniliparus gen. nov., with descriptions of Segniliparus rotundus sp. nov. and Segniliparus rugosus sp. nov.. Int J Syst Evol Microbiol 55:, 1615–1624. [CrossRef][PubMed]
    [Google Scholar]
  3. Collins M. D., Jones D.. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2, 4-diaminobutyric acid. . Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  4. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  8. Gonzalez C., Gutierrez C., Ramirez C.. ( 1978;). Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. . Can J Microbiol 24:, 710–715. [CrossRef][PubMed]
    [Google Scholar]
  9. Goodfellow M., Maldonado L. A.. ( 2006;). The families Dietziaceae, Gordoniaceae, Nocardiaceae and Tsukamurellaceae. . In The Prokaryotes. A Handbook on the Biology of Bacteria, , 3rd edn., vol. 3, pp. 843–888. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E... New York:: Springer;. [CrossRef]
    [Google Scholar]
  10. Hasegawa T., Takizawa M., Tanida S.. ( 1983;). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  11. Hayakawa M., Nonomura H.. ( 1987;). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65:, 501–509. [CrossRef]
    [Google Scholar]
  12. Katayama T., Kato T., Tanaka M., Douglas T. A., Brouchkov A., Abe A., Sone T., Fukuda M., Asano K.. ( 2010;). Tomitella biformata gen. nov., sp. nov., a new member of the suborder Corynebacterineae isolated from a permafrost ice wedge. . Int J Syst Evol Microbiol 60:, 2803–2807. [CrossRef][PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  14. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  15. Kovacs N.. ( 1956;). Identification of Pseudomonas pyocyanea by the oxidase reaction. . Nature 178:, 703–704. [CrossRef][PubMed]
    [Google Scholar]
  16. Kroppenstedt R. M.. ( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H. A.. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  19. Minnikin D. E., Alshamaony L., Goodfellow M.. ( 1975;). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. . J Gen Microbiol 88:, 200–204. [CrossRef][PubMed]
    [Google Scholar]
  20. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  22. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  23. Soddell J. A., Stainsby F. M., Eales K. L., Kroppenstedt R. M., Seviour R. J., Goodfellow M.. ( 2006;). Millisia brevis gen. nov., sp. nov., an actinomycete isolated from activated sludge foam. . Int J Syst Evol Microbiol 56:, 739–744. [CrossRef][PubMed]
    [Google Scholar]
  24. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  26. Tang S. K., Wang Y., Chen Y., Lou K., Cao L. L., Xu L. H., Li W. J.. ( 2009;). Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. . Int J Syst Evol Microbiol 59:, 2025–2032. [CrossRef][PubMed]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  28. Uchida K., Kudo T., Suzuki K. I., Nakase T.. ( 1999;). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. . J Gen Appl Microbiol 45:, 49–56. [CrossRef][PubMed]
    [Google Scholar]
  29. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L.. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.060202-0
Loading
/content/journal/ijsem/10.1099/ijs.0.060202-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error