1887

Abstract

A Gram-reaction-negative, yellow-pigmented, strictly aerobic bacterium, designated M0116, was isolated from the sediment of the Mohe Basin in north-east China. Flexirubin-type pigments were produced. Cells were catalase- and oxidase-positive and non-gliding rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M0116 was a member of the family and was most closely related to members of the genera , and with 90.5–91.0 % sequence similarities. The major cellular fatty acids were iso-C and iso-C 3-OH. The major respiratory quinone was MK-6 and the major polar lipid was phosphatidylethanolamine. The DNA G+C content was 38.2 mol%. Based on phenotypic, phylogenetic and genotypic data, strain M0116 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is M0116 ( = CGMCC 1.12708 = JCM 19634). Emended descriptions of , and are also proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.060178-0
2014-05-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/5/1481.html?itemId=/content/journal/ijsem/10.1099/ijs.0.060178-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A.. (editors) ( 1993;). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F.. ( 2011;). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 106–111. Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D., Parte A... New York:: Springer;.
    [Google Scholar]
  3. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P.. ( 1996;). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). . Int J Syst Bacteriol 46:, 128–148. [CrossRef]
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  5. Colwell F. S., Stormberg G. J., Phelps T. J., Birnbaum S. A., McKinley J., Rawson S. A., Veverka C., Goodwin S., Long P. E.. & other authors ( 1992;). Innovative techniques for collection of saturated and unsaturated subsurface basalts and sediments for microbiological characterization. . J Microbiol Methods 15:, 279–292. [CrossRef]
    [Google Scholar]
  6. Da Costa M. S., Albuquerque L., Nobre M. F., Wait R.. ( 2011a;). The extraction and identification of respiratory lipoquinones of prokaryotes and their use in taxonomy. . Method Microbiol 38:, 197–206. [CrossRef]
    [Google Scholar]
  7. Da Costa M. S., Albuquerque L., Nobre M. F., Wait R.. ( 2011b;). The identification of polar lipids in prokaryotes. . Method Microbiol 38:, 165–181. [CrossRef]
    [Google Scholar]
  8. Dees S. B., Moss C. W., Hollis D. G., Weaver R. E.. ( 1986;). Chemical characterization of Flavobacterium odoratum, Flavobacterium breve, and Flavobacterium-like groups IIe, IIh, and IIf. . J Clin Microbiol 23:, 267–273.[PubMed]
    [Google Scholar]
  9. Dong K., Chen F., Du Y., Wang G.. ( 2013;). Flavobacterium enshiense sp. nov., isolated from soil, and emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense. . Int J Syst Evol Microbiol 63:, 886–892. [CrossRef][PubMed]
    [Google Scholar]
  10. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  11. Hollis D. G., Daneshvar M. I., Moss C. W., Baker C. N.. ( 1995;). Phenotypic characteristics, fatty acid composition, and isoprenoid quinone content of CDC group IIg bacteria. . J Clin Microbiol 33:, 762–764.[PubMed]
    [Google Scholar]
  12. Holmes B., Snell J. J. S., Lapage S. P.. ( 1978;). Revised description, from clinical strains, of Flavobacterium breve (Lustig) Bergey et al. 1923 and proposal of the neotype strain. . Int J Syst Bacteriol 28:, 201–208. [CrossRef]
    [Google Scholar]
  13. Holmes B., Steigerwalt A. G., Weaver R. E., Brenner D. J.. ( 1986;). Weeksella virosa gen. nov., sp. nov. (Formerly group IIf), found in human clinical specimens. . Syst Appl Microbiol 8:, 185–190. [CrossRef]
    [Google Scholar]
  14. Jooste P. J.. ( 1985;). The taxonomy and significance of Flavobacterium–Cytophaga strains from dairy sources. PhD thesis, University of the Orange Free State;, Bloemfontein, South Africa:.
    [Google Scholar]
  15. Kämpfer P., Avesani V., Janssens M., Charlier J., De Baere T., Vaneechoutte M.. ( 2006;). Description of Wautersiella falsenii gen. nov., sp. nov., to accommodate clinical isolates phenotypically resembling members of the genera Chryseobacterium and Empedobacter. . Int J Syst Evol Microbiol 56:, 2323–2329. [CrossRef][PubMed]
    [Google Scholar]
  16. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  17. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  18. Kishino H., Hasegawa M.. ( 1989;). Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea. . J Mol Evol 29:, 170–179. [CrossRef][PubMed]
    [Google Scholar]
  19. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M... Chichester:: Wiley;.
    [Google Scholar]
  20. Li C., Lai Q., Fu Y., Chen S., Shao Z.. ( 2013;). Galbibacter marinus sp. nov., isolated from deep-sea sediment. . Int J Syst Evol Microbiol 63:, 1427–1430. [CrossRef][PubMed]
    [Google Scholar]
  21. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  22. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  23. Murray R., Doetsch R. N., Robinow C. F.. ( 1994;). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  24. Reichenbach H.. ( 1992;). Flavobacteriaceae fam. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List No. 41. . Int J Syst Bacteriol 42:, 327–329. [CrossRef]
    [Google Scholar]
  25. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  26. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  27. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  28. Surendra V., Bhawana P., Suresh K., Srinivas T. N., Anil Kumar P.. ( 2012;). Imtechella halotolerans gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from estuarine water. . Int J Syst Evol Microbiol 62:, 2624–2630. [CrossRef][PubMed]
    [Google Scholar]
  29. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  30. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  31. Vandamme P., Bernardet J.-F., Segers P., Kersters K., Holmes B.. ( 1994;). New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev.. Int J Syst Bacteriol 44:, 827–831. [CrossRef]
    [Google Scholar]
  32. Yu T., Yin Q., Song X., Zhao R., Shi X., Zhang X. H.. ( 2013;). Aquimarina longa sp. nov., isolated from seawater, and emended description of Aquimarina muelleri. . Int J Syst Evol Microbiol 63:, 1235–1240. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.060178-0
Loading
/content/journal/ijsem/10.1099/ijs.0.060178-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error