1887

Abstract

Two thermophilic, strictly anaerobic, Gram-negative bacteria, designated strains AZM34c06 and AZM44c09, were isolated from terrestrial hot springs in Japan. The optimum growth conditions for strain AZM34c06 were 60 °C, pH 7.4 and 0 % additional NaCl, and those for strain AZM44c09 were 70 °C, pH 7.4 and 0 % additional NaCl. Complete genome sequencing was performed for both strains, revealing genome sizes of 2.19 Mbp (AZM34c06) and 2.01 Mbp (AZM44c09). Phylogenetic analyses based on 16S rRNA gene sequences and the concatenated predicted amino acid sequences of 33 ribosomal proteins showed that both strains belonged to the genus . The closest relatives of strains AZM34c06 and AZM44c09 were the type strains of (96.0 % similarity based on the 16S rRNA gene and 84.1 % similarity based on ribosomal proteins) and (98.6 and 92.7 % similarity), respectively. Using , the average nucleotide identity was 70.4–70.5 % when comparing strain AZM34c06 and TMO and 76.6 % when comparing strain AZM44c09 and NBRC 106472. Both values are far below the 95 % threshold value for species delineation. In view of these data, we propose the inclusion of the two isolates in the genus within two novel species, sp. nov. (type strain AZM34c06 = NBRC 106115 = DSM 23275) and sp. nov. (type strain AZM44c09 = NBRC 106116 = DSM 23272).

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2014-06-01
2021-08-04
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