sp. nov., a xylanolytic, ustilaginomycetous yeast species isolated from an insect pest of sugarcane roots Free

Abstract

A novel ustilaginomycetous yeast isolated from the intestinal tract of an insect pest of sugarcane roots in Ribeirão Preto, São Paulo State, Brazil, represents a novel species of the genus based on molecular analyses of the D1/D2 rDNA large subunit and the internal transcribed spacer (ITS1+ITS2) regions. The name sp. nov. is proposed for this species, with GHG001 ( = CBS 13268 = UFMG-CM-Y307) as the type strain. sp. nov. is a sister species of , originally isolated from leaves of vetiver grass and sugarcane in Thailand. sp. nov. is able to grow well with xylan as the sole carbon source and produces high levels of an endo-1,4-xylanase that has a higher specific activity in comparison with other eukaryotic xylanases. This enzyme has a variety of industrial applications, indicating the great biotechnological potential of .

Funding
This study was supported by the:
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (Award 10/513224-2)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Fundação do Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)
  • Vale S.A.
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2014-06-01
2024-03-28
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References

  1. Adsul M. G., Bastawde K. B., Gokhale D. V. ( 2009 ). Biochemical characterization of two xylanases from yeast Pseudozyma hubeiensis producing only xylooligosaccharides. . Bioresour Technol 100, 64886495. [View Article] [PubMed]
    [Google Scholar]
  2. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403410. [View Article] [PubMed]
    [Google Scholar]
  3. Avis T. J., Bélanger R. R. ( 2002 ). Mechanisms and means of detection of biocontrol activity of Pseudozyma yeasts against plant-pathogenic fungi. . FEMS Yeast Res 2, 58.[PubMed]
    [Google Scholar]
  4. Bakkeren G., Kronstad J. W. ( 1994 ). Linkage of mating-type loci distinguishes bipolar from tetrapolar mating in basidiomycetous smut fungi. . Proc Natl Acad Sci U S A 91, 70857089. [View Article] [PubMed]
    [Google Scholar]
  5. Boekhout T. ( 2011 ). Pseudozyma Bandoni emend. Boekhout (1985) and a comparison with the yeast state of Ustilago maydis (De Candolle) Corda (1842). . In The Yeasts, a Taxonomic Study, , 5th edn., vol. 3, pp. 18571868. Edited by Kurtzman C. P., Fell J. W., Boekhout T. . Amsterdam:: Elsevier;. [View Article]
    [Google Scholar]
  6. Borges T. A., Souza A. T., Squina F. M., Riaño-Pachón D. M., Santos R. A. C., Machado E., Oliveira J. V. C., Damásio R. L., Goldman G. H. ( 2014 ). Biochemical characterization of an endoxylanase from Pseudozyma brasiliensis sp. nov. strain GHG001 isolated from the intestinal tract of Chrysomelidae larvae associated to sugarcane roots. . Process Biochem 49, 7783. [View Article]
    [Google Scholar]
  7. Bussamara R., Fuentefria A. M., de Oliveira E. S., Broetto L., Simcikova M., Valente P., Schrank A., Vainstein M. H. ( 2010 ). Isolation of a lipase-secreting yeast for enzyme production in a pilot-plant scale batch fermentation. . Bioresour Technol 101, 268275. [View Article] [PubMed]
    [Google Scholar]
  8. Bussamara R., Dall’agnol L., Schrank A., Fernandes K. F., Vainstein M. H. ( 2012 ). Optimal conditions for continuous immobilization of Pseudozyma hubeiensis (strain HB85A) lipase by adsorption in a packed-bed reactor by response surface methodology. . Enzyme Res 2012, 329178. [View Article] [PubMed]
    [Google Scholar]
  9. Casselton L. A. ( 2002 ). Mate recognition in fungi. . Heredity (Edinb) 88, 142147. [View Article] [PubMed]
    [Google Scholar]
  10. Casselton L. A., Olesnicky N. S. ( 1998 ). Molecular genetics of mating recognition in basidiomycete fungi. . Microbiol Mol Biol Rev 62, 5570.[PubMed]
    [Google Scholar]
  11. Chamnanpa T., Limtong P., Srisuk N., Limtong S. ( 2013 ). Pseudozyma vetiver sp. nov., a novel anamorphic ustilaginomycetous yeast species isolated from the phylloplane in Thailand. . Antonie van Leeuwenhoek 104, 637644. [View Article] [PubMed]
    [Google Scholar]
  12. Fell J. W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. ( 2000 ). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. . Int J Syst Evol Microbiol 50, 13511371. [View Article] [PubMed]
    [Google Scholar]
  13. Golubev V. I. ( 2007 ). [Mycocinogeny in smut yeast-like fungi of the genus Pseudozyma]. . Mikrobiologiia 76, 813816 (in Russian).[PubMed]
    [Google Scholar]
  14. Hartmann H. A., Krüger J., Lottspeich F., Kahmann R. ( 1999 ). Environmental signals controlling sexual development of the corn smut fungus Ustilago maydis through the transcriptional regulator Prf1. . Plant Cell 11, 12931306. [View Article] [PubMed]
    [Google Scholar]
  15. Higgs P. G. ( 2000 ). RNA secondary structure: physical and computational aspects. . Q Rev Biophys 33, 199253. [View Article] [PubMed]
    [Google Scholar]
  16. Hudelot C., Gowri-Shankar V., Jow H., Rattray M., Higgs P. G. ( 2003 ). RNA-based phylogenetic methods: application to mammalian mitochondrial RNA sequences. . Mol Phylogenet Evol 28, 241252. [View Article] [PubMed]
    [Google Scholar]
  17. Jow H., Hudelot C., Rattray M., Higgs P. G. ( 2002 ). Bayesian phylogenetics using an RNA substitution model applied to early mammalian evolution. . Mol Biol Evol 19, 15911601. [View Article] [PubMed]
    [Google Scholar]
  18. Kämper J., Kahmann R., Bölker M., Ma L. J., Brefort T., Saville B. J., Banuett F., Kronstad J. W., Gold S. E. & other authors ( 2006 ). Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis . . Nature 444, 97101. [View Article] [PubMed]
    [Google Scholar]
  19. Koetschan C., Hackl T., Müller T., Wolf M., Förster F., Schultz J. ( 2012 ). ITS2 database IV: interactive taxon sampling for internal transcribed spacer 2 based phylogenies. . Mol Phylogenet Evol 63, 585588. [View Article] [PubMed]
    [Google Scholar]
  20. Konishi M., Nagahama T., Fukuoka T., Morita T., Imura T., Kitamoto D., Hatada Y. ( 2011 ). Yeast extract stimulates production of glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma hubeiensis SY62. . J Biosci Bioeng 111, 702705. [View Article] [PubMed]
    [Google Scholar]
  21. Konishi M., Hatada Y., Horiuchi J. ( 2013 ). Draft Genome Sequence of the Basidiomycetous Yeast-Like Fungus Pseudozyma hubeiensis SY62, Which Produces an Abundant Amount of the Biosurfactant Mannosylerythritol Lipids. . Genome Announc. 1: e00409-13. [CrossRef]
    [Google Scholar]
  22. Kück P., Meusemann K. ( 2010 ). FASconCAT: Convenient handling of data matrices. . Mol Phylogenet Evol 56, 11151118. [View Article] [PubMed]
    [Google Scholar]
  23. Kurtzman C. P., Fell J. W., Boekhout T., Robert V. ( 2011 ). Methods for isolation, phenotypic characterization and maintenance of yeasts. . In The Yeasts, a Taxonomic Study, , 5th edn., vol. 1, pp. 87110. Edited by Kurtzman C. P., Fell J. W., Boekhout T. . Amsterdam:: Elsevier;. [View Article]
    [Google Scholar]
  24. Lefebvre F., Joly D. L., Labbé C., Teichmann B., Linning R., Belzile F., Bakkeren G., Bélanger R. R. ( 2013 ). The transition from a phytopathogenic smut ancestor to an anamorphic biocontrol agent deciphered by comparative whole-genome analysis. . Plant Cell 25, 19461959. [View Article] [PubMed]
    [Google Scholar]
  25. Li L., Stoeckert C. J. Jr, Roos D. S. ( 2003 ). OrthoMCL: identification of ortholog groups for eukaryotic genomes. . Genome Res 13, 21782189. [View Article] [PubMed]
    [Google Scholar]
  26. Liou G. Y., Wei Y. H., Lin S. J., Wen C. Y., Lee F. L. ( 2009 ). Pseudozyma pruni sp. nov., a novel ustilaginomycetous anamorphic fungus from flowers in Taiwan. . Int J Syst Evol Microbiol 59, 18131817. [View Article] [PubMed]
    [Google Scholar]
  27. Lorenz S., Guenther M., Grumaz C., Rupp S., Zibek S., Sohn K. ( 2014 ). Genome sequence of the basidiomycetous fungus Pseudozyma aphidis DSM70725, an efficient producer of biosurfactant mannosylerythritol lipids. . Genome Announc 2, e00053e00014. [View Article] [PubMed]
    [Google Scholar]
  28. Marchand G., Rémus-Borel W., Chain F., Hammami W., Belzile F., Bélanger R. R. ( 2009 ). Identification of genes potentially involved in the biocontrol activity of Pseudozyma flocculosa . . Phytopathology 99, 11421149. [View Article] [PubMed]
    [Google Scholar]
  29. Mekha N., Takashima M., Boon-Long J., Cho O., Sugita T. ( 2014 ). Three new basidiomycetous yeasts, Pseudozyma alboarmeniaca sp. nov., Pseudozyma crassa sp. nov., Pseudozyma siamensis sp. nov. isolated from Thai patients. . Microbiol Immunol 58, 914. [View Article] [PubMed]
    [Google Scholar]
  30. Morita T., Fukuoka T., Konishi M., Imura T., Yamamoto S., Kitagawa M., Sogabe A., Kitamoto D. ( 2009 ). Production of a novel glycolipid biosurfactant, mannosylmannitol lipid, by Pseudozyma parantarctica and its interfacial properties. . Appl Microbiol Biotechnol 83, 10171025. [View Article] [PubMed]
    [Google Scholar]
  31. Morita T., Takashima M., Fukuoka T., Konishi M., Imura T., Kitamoto D. ( 2010 ). Isolation of basidiomycetous yeast Pseudozyma tsukubaensis and production of glycolipid biosurfactant, a diastereomer type of mannosylerythritol lipid-B. . Appl Microbiol Biotechnol 88, 679688. [View Article] [PubMed]
    [Google Scholar]
  32. Morita T., Ogura Y., Takashima M., Hirose N., Fukuoka T., Imura T., Kondo Y., Kitamoto D. ( 2011 ). Isolation of Pseudozyma churashimaensis sp. nov., a novel ustilaginomycetous yeast species as a producer of glycolipid biosurfactants, mannosylerythritol lipids. . J Biosci Bioeng. 112:13744. [CrossRef]
    [Google Scholar]
  33. Morita T., Koike H., Koyama Y., Hagiwara H., Ito E., Fukuoka T., Imura T., Machida M., Kitamoto D. ( 2013 ). Genome Sequence of the Basidiomycetous Yeast Pseudozyma antarctica T-34, a Producer of the Glycolipid Biosurfactants Mannosylerythritol Lipids. . Genome Announc. 1:e0006413. [CrossRef]
    [Google Scholar]
  34. Moure A., Gullón P., Domínguez H., Parajo J. C. ( 2006 ). Advances in the manufacture, purification and applications of xylo-oligosaccharides as food additives and nutraceuticals. . Process Biochem 41, 19131923. [View Article]
    [Google Scholar]
  35. Oliveira J. V., Dos Santos R. A., Borges T. A., Riaño-Pachón D. M., Goldman G. H. ( 2013 ). Draft genome sequence of Pseudozyma brasiliensis sp. nov. strain GHG001, a high producer of endo-1,4-xylanase isolated from an insect pest of sugarcane. . Genome Announc 1, e00920e00913. [View Article] [PubMed]
    [Google Scholar]
  36. Polizeli M. L., Rizzatti A. C., Monti R., Terenzi H. F., Jorge J. A., Amorim D. S. ( 2005 ). Xylanases from fungi: properties and industrial applications. . Appl Microbiol Biotechnol 67, 577591. [View Article] [PubMed]
    [Google Scholar]
  37. Rice P., Longden I., Bleasby A. ( 2000 ). emboss: the European Molecular Biology Open Software Suite. . Trends Genet 16, 276277. [View Article] [PubMed]
    [Google Scholar]
  38. Sambrook J., Russell D. W. ( 2001 ). Molecular Cloning: a Laboratory Manual, , 3rd edn.. New York:: Cold Spring Harbor Laboratory Press;.
    [Google Scholar]
  39. Seo H. S., Um H. J., Min J., Rhee S. K., Cho T. J., Kim Y. H., Lee J. ( 2007 ). Pseudozyma jejuensis sp. nov., a novel cutinolytic ustilaginomycetous yeast species that is able to degrade plastic waste. . FEMS Yeast Res 7, 10351045. [View Article] [PubMed]
    [Google Scholar]
  40. Smith T. F., Waterman M. S. ( 1981 ). Identification of common molecular subsequences. . J Mol Biol 147, 195197. [View Article] [PubMed]
    [Google Scholar]
  41. Smith D. G., Garcia-Pedrajas M. D., Hong W., Yu Z., Gold S. E., Perlin M. H. ( 2004 ). An ste20 homologue in Ustilago maydis plays a role in mating and pathogenicity. . Eukaryot Cell 3, 180189. [View Article] [PubMed]
    [Google Scholar]
  42. Stamatakis A. ( 2006 ). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22, 26882690. [View Article] [PubMed]
    [Google Scholar]
  43. Statzell-Tallman A., Scorzetti G., Fell J. W. ( 2010 ). Candida spencermartinsiae sp. nov., Candida taylorii sp. nov. and Pseudozyma abaconensis sp. nov., novel yeasts from mangrove and coral reef ecosystems. . Int J Syst Evol Microbiol 60, 19781984. [View Article] [PubMed]
    [Google Scholar]
  44. Sugita T., Takashima M., Poonwan N., Mekha N., Malaithao K., Thungmuthasawat B., Prasarn S., Luangsook P., Kudo T. ( 2003 ). The first isolation of ustilaginomycetous anamorphic yeasts, Pseudozyma species, from patients’ blood and a description of two new species: P. parantarctica and P. thailandica . . Microbiol Immunol 47, 183190. [View Article] [PubMed]
    [Google Scholar]
  45. Tabei Y., Kiryu H., Kin T., Asai K. ( 2008 ). A fast structural multiple alignment method for long RNA sequences. . BMC Bioinformatics 9, 33. [View Article] [PubMed]
    [Google Scholar]
  46. Urban M., Kahmann R., Bölker M. ( 1996 ). The biallelic a mating type locus of Ustilago maydis: remnants of an additional pheromone gene indicate evolution from a multiallelic ancestor. . Mol Gen Genet 250, 414420. [View Article] [PubMed]
    [Google Scholar]
  47. Wang Q. M., Jia J. H., Bai F. Y. ( 2006 ). Pseudozyma hubeiensis sp. nov. and Pseudozyma shanxiensis sp. nov., novel ustilaginomycetous anamorphic yeast species from plant leaves. . Int J Syst Evol Microbiol 56, 289293. [View Article] [PubMed]
    [Google Scholar]
  48. Xu J., Saunders C. W., Hu P., Grant R. A., Boekhout T., Kuramae E. E., Kronstad J. W., Deangelis Y. M., Reeder N. L. & other authors ( 2007 ). Dandruff-associated Malassezia genomes reveal convergent and divergent virulence traits shared with plant and human fungal pathogens. . Proc Natl Acad Sci U S A 104, 1873018735. [View Article] [PubMed]
    [Google Scholar]
  49. Yang Z. ( 1996 ). Among-site rate variation and its impact on phylogenetic analyses. . Trends Ecol Evol 11, 367372. [View Article] [PubMed]
    [Google Scholar]
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