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Abstract

A novel actinobacterium, designated IPBSL-7, was isolated from a drilling core 297 m deep obtained from the Iberian Pyrite Belt. The strain was isolated anaerobically using nitrate as the electron acceptor. 16S rRNA gene sequence analysis revealed that it was related to SST-39 (95.7 % similarity), Ben 106 (95.7 %), KSS-17Se (95.6 %) and SL014B-20A1 (95.0 %), while its similarity to any other member of the family was less than 94 %. Cells were non-motile, non-spore-forming, Gram-positive, oval to rod-shaped, and often appeared in pairs or small groups. The strain was facultatively anaerobic, oxidase-negative, catalase-positive and capable of reducing nitrate. Colonies were circular, convex, smooth and colourless. The organism could grow at between 15 and 40 °C, with an optimal growth at 37 °C. The pH range for growth was from pH 6 to 9, with pH 8 being the optimal value. Strain IPBSL-7 had peptidoglycan type A3-γ′, with -diaminopimelic acid as the diagnostic diamino-acid and glycine at position 1 of the peptide subunit. The dominant menaquinone was MK-9(H) (93.8 %). The major cellular fatty acid was anteiso-C (55.0 %). The DNA G+C content was 70.3 mol%. On the basis of phenotypic and phylogenetic results, strain IPBSL-7 can be differentiated from previously described species of the genus and, therefore, represents a novel species, for which the name sp. nov. is proposed. The type strain is IPBSL-7 ( = CECT 8385 = DSM 27266).

Funding
This study was supported by the:
  • European Research Council (ERC) (Award 250350)
  • Spanish Ministerio de Economía y Competitividad (MINECO) (Award AYA2011-24803)
  • Spanish Consejo Superior de Investigaciones Científicas (CSIC)
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2014-10-01
2021-10-17
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