1887

Abstract

Strains CUPV261 and CUPV262 were isolated from ropy natural ciders of the Basque Country, Spain, in 2007. Cells are Gram-stain positive, non-spore-forming, motile rods, facultative anaerobes and catalase-negative. The strains are obligately homofermentative (final product -lactate) and produce exopolysaccharides from sucrose. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the highest similarity to both isolates corresponded to the type strain of (99.1 %), followed by (96.4 %), and (96.2 %), and for all other established species, 16S rRNA gene sequence similarities were below 96 %. The species delineation of strains CUPV261 and CUPV262 was evaluated through RAPD fingerprinting. In addition, a random partial genome pyrosequencing approach was performed on strain CUPV261 in order to compare it with the genome sequence of DSM 20605 and calculate indexes of average nucleotide identity (ANI) between them. Results permit the conclusion that strains CUPV261 and CUPV262 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CUPV261 ( = CECT 8227 = KCTC 21012).

Funding
This study was supported by the:
  • Spanish Ministry of Science and Innovation (Award CSD2007-00063, AGL2012-40084-C03 and AGL2009-12998-C03)
  • Basque Government (Award IT335-10)
  • Generalitat Valenciana (Award PROMETEO/2012/040)
  • ‘Gobierno Vasco, Dpto. Agricultura, Pesca y Alimentación’
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.059980-0
2014-09-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/9/2949.html?itemId=/content/journal/ijsem/10.1099/ijs.0.059980-0&mimeType=html&fmt=ahah

References

  1. Arahal D. R., Sánchez E., Macián M. C., Garay E. ( 2008 ). Value of recN sequences for species identification and as a phylogenetic marker within the family “Leuconostocaceae”. . Int Microbiol 11, 3339.[PubMed]
    [Google Scholar]
  2. Beech F. W., Carr J. G. ( 1977 ). Cider and Perry. . In Alcoholic Beverages, vol. 1, pp.139313. Edited by Rose A. H. . London:: Academic Press;.
    [Google Scholar]
  3. Carr J. G., Davies P. A. ( 1970 ). Homofermentative Lactobacilli of ciders including Lactobacillus mali nov. spec. . J Appl Bacteriol 33, 768774. [View Article] [PubMed]
    [Google Scholar]
  4. Carr J. G., Davies P. A. ( 1972 ). The ecology and classification of strains of Lactobacillus collinoides nov. spec.: a bacterium commonly found in fermenting apple juice. . J Appl Bacteriol 35, 463471. [View Article] [PubMed]
    [Google Scholar]
  5. Claisse O., Lonvaud-Funel A. ( 2000 ). Assimilation of glycerol by a strain of Lactobacillus collinoides isolated from cider. . Food Microbiol 17, 513519. [View Article]
    [Google Scholar]
  6. Dellaglio F., Torriani S., Felis G. E. ( 2004 ). Reclassification of Lactobacillus cellobiosus Rogosa et al. 1953 as a later synonym of Lactobacillus fermentum Beijerinck 1901. . Int J Syst Evol Microbiol 54, 809812. [View Article] [PubMed]
    [Google Scholar]
  7. Dueñas M., Irastorza A., Fernandez K., Bilbao A., Huerta A. ( 1994 ). Microbial populations and malolactic fermentation of apple cider using traditional and modified methods. . J Food Sci 59, 10601064. [View Article]
    [Google Scholar]
  8. Dueñas M., Irastorza A., Fernandez K., Bilbao A. ( 1995 ). Heterofermentative lactobacilli causing ropiness in Basque Country ciders. . J Food Prot 58, 7680.
    [Google Scholar]
  9. Dueñas-Chasco M. T., Rodríguez-Carvajal M. A., Tejero Mateo P., Franco-Rodríguez G., Espartero J. L., Irastorza-Iribas A., Gil-Serrano A. M. ( 1997 ). Structural analysis of the exopolysaccharide produced by Pediococcus damnosus 2.6. . Carbohydr Res 303, 453458. [View Article] [PubMed]
    [Google Scholar]
  10. Dueñas-Chasco M. T., Rodríguez-Carvajal M. A., Tejero-Mateo P., Espartero J. L., Irastorza-Iribas A., Gil-Serrano A. M. ( 1998 ). Structural analysis of the exopolysaccharides produced by Lactobacillus spp. G-77. . Carbohydr Res 307, 125133. [View Article] [PubMed]
    [Google Scholar]
  11. Edwards C. G., Collins M. D., Lawson P. A., Rodriguez A. V. ( 2000 ). Lactobacillus nagelii sp. nov., an organism isolated from a partially fermented wine. . Int J Syst Evol Microbiol 50, 699702. [View Article] [PubMed]
    [Google Scholar]
  12. Ehrmann M. A., Müller M. R. A., Vogel R. F. ( 2003 ). Molecular analysis of sourdough reveals Lactobacillus mindensis sp. nov.. Int J Syst Evol Microbiol 53, 713. [View Article] [PubMed]
    [Google Scholar]
  13. Endo A., Okada S. ( 2005 ). Lactobacillus satsumensis sp. nov., isolated from mashes of shochu, a traditional Japanese distilled spirit made from fermented rice and other starchy materials. . Int J Syst Evol Microbiol 55, 8385. [View Article] [PubMed]
    [Google Scholar]
  14. Garai G., Dueñas M. T., Irastorza A., Moreno-Arribas M. V. ( 2007 ). Biogenic amine production by lactic acid bacteria isolated from cider. . Lett Appl Microbiol 45, 473478. [View Article] [PubMed]
    [Google Scholar]
  15. Garai-Ibabe G., Dueñas M. T., Irastorza A., Sierra-Filardi E., Werning M. L., López P., Corbí A. L., Fernández de Palencia P. ( 2010 ). Naturally occurring 2-substituted (1,3)-beta-D-glucan producing Lactobacillus suebicus and Pediococcus parvulus strains with potential utility in the production of functional foods. . Bioresour Technol 101, 92549263. [View Article] [PubMed]
    [Google Scholar]
  16. Ibarburu I., Soria-Díaz M. E., Rodríguez-Carvajal M. A., Velasco S. E., Tejero-Mateo P., Gil-Serrano A. M., Irastorza A., Dueñas M. T. ( 2007 ). Growth and exopolysaccharide (EPS) production by Oenococcus oeni I4 and structural characterization of their EPSs. . J Appl Microbiol 103, 477486. [View Article] [PubMed]
    [Google Scholar]
  17. Kaneuchi C., Seki M., Komagata K. ( 1988 ). Taxonomic study of Lactobacillus mali Carr and Davis 1970 and related strains: validation of Lactobacillus mali Carr and Davis 1970 over Lactobacillus yamanashiensis Nomomura 1983. . Int J Syst Bacteriol 38, 269272. [View Article]
    [Google Scholar]
  18. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  19. Kleynmans U., Heinzl H., Hammes W. P. ( 1989 ). Lactobacillus suebicus sp. nov., an obligately heterofermentative Lactobacillus species isolated from fruit mashes. . Syst Appl Microbiol 11, 267271. [View Article]
    [Google Scholar]
  20. Laplace J. M., Jacquet A., Travers I., Simon J. P., Auffray Y. ( 2001 ). Incidence of land and physicochemical composition of apples on the qualitative and quantitative development of microbial flora during cider fermentations. . J Inst Brew 107, 227233. [View Article]
    [Google Scholar]
  21. Lucena T., Ruvira M. A., Arahal D. R., Macián M. C., Pujalte M. J. ( 2012a ). Vibrio aestivus sp. nov. and Vibrio quintilis sp. nov., related to Marisflavi and Gazogenes clades, respectively. . Syst Appl Microbiol 35, 427431. [View Article] [PubMed]
    [Google Scholar]
  22. Lucena T., Pujalte M. J., Ruvira M. A., Garay E., Macián M. C., Arahal D. R. ( 2012b ). Tropicibacter multivorans sp. nov., an aerobic alphaproteobacterium isolated from surface seawater. . Int J Syst Evol Microbiol 62, 844848. [View Article] [PubMed]
    [Google Scholar]
  23. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  24. Mañes-Lázaro R., Ferrer S., Rosselló-Mora R., Pardo I. ( 2008 ). Lactobacillus uvarum sp. nov.–a new lactic acid bacterium isolated from Spanish Bobal grape must. . Syst Appl Microbiol 31, 425433. [View Article] [PubMed]
    [Google Scholar]
  25. Mañes-Lázaro R., Ferrer S., Rosselló-Mora R., Pardo I. ( 2009 ). Lactobacillus oeni sp. nov., from wine. . Int J Syst Evol Microbiol 59, 20102014. [View Article] [PubMed]
    [Google Scholar]
  26. Marieta C., Ibarburu I., Dueñas M., Irastorza A. ( 2009 ). Supramolecular structure and conformation of a (1→3)(1→2)-β-d-glucan from Lactobacillus suebicus CUPV221 as observed by tapping mode atomic force microscopy. . J Agric Food Chem 57, 61836188. [View Article] [PubMed]
    [Google Scholar]
  27. Marshall C. R., Walkley V. T. ( 1951 ). Some aspects of microbiology applied to commercial apple juice production. I. Distribution of microorganisms on the fruit. . J Food Sci 16, 448456. [View Article]
    [Google Scholar]
  28. Meier-Kolthoff J. P., Auch A. F., Klenk H.-P., Göker M. ( 2013 ). Genome sequence-based species delimitation with confidence intervals and improved distance functions. . BMC Bioinformatics 14, 60. [View Article] [PubMed]
    [Google Scholar]
  29. Nielsen D. S., Schillinger U., Franz C. M., Bresciani J., Amoa-Awua W., Holzapfel W. H., Jakobsen M. ( 2007 ). Lactobacillus ghanensis sp. nov., a motile lactic acid bacterium isolated from Ghanaian cocoa fermentations. . Int J Syst Evol Microbiol 57, 14681472. [View Article] [PubMed]
    [Google Scholar]
  30. Pinto B., Chenoll E., Aznar R. ( 2005 ). Identification and typing of food-borne Staphylococcus aureus by PCR-based techniques. . Syst Appl Microbiol 28, 340352. [View Article] [PubMed]
    [Google Scholar]
  31. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. ( 2007 ). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. . Nucleic Acids Res 35, 71887196. [View Article] [PubMed]
    [Google Scholar]
  32. Richter M., Rosselló-Móra R. ( 2009 ). Shifting the genomic gold standard for the prokaryotic species definition. . Proc Natl Acad Sci U S A 106, 1912619131. [View Article] [PubMed]
    [Google Scholar]
  33. Rodas A. M., Chenoll E., Macián M. C., Ferrer S., Pardo I., Aznar R. ( 2006 ). Lactobacillus vini sp. nov., a wine lactic acid bacterium homofermentative for pentoses. . Int J Syst Evol Microbiol 56, 513517. [View Article] [PubMed]
    [Google Scholar]
  34. Ruas-Madiedo P., Abraham A. G., Mozzi F., de los Reyes-Gavilán C. G. ( 2008 ). Functionality of exopolysaccharides produced by lactic acid bacteria. . In Molecular Aspects of Lactic Acid Bacteria for Traditional and New Applications, pp. 137166. Edited by Mayo B., López P., Pérez-Martínez G. . Kerala, India:: Research Signpost;.
    [Google Scholar]
  35. Salih A. G., Le Quéré J. M., Drilleau J. F., Moreno J. ( 1990 ). Lactic acid bacteria and malolactic fermentation in the manufacture of Spanish cider. . J Inst Brew 96, 369372. [View Article]
    [Google Scholar]
  36. Sauvageot N., Gouffi K., Laplace J. M., Auffray Y. ( 2000 ). Glycerol metabolism in Lactobacillus collinoides: production of 3-hydroxypropionaldehyde, a precursor of acrolein. . Int J Food Microbiol 55, 167170. [View Article] [PubMed]
    [Google Scholar]
  37. Simpson P. J., Stanton C., Fitzgerald G. F., Ross R. P. ( 2002 ). Genomic diversity within the genus Pediococcus as revealed by randomly amplified polymorphic DNA PCR and pulsed-field gel electrophoresis. . Appl Environ Microbiol 68, 765771. [View Article] [PubMed]
    [Google Scholar]
  38. Velasco S. E., Areizaga J., Irastorza A., Dueñas M. T., Santamaría A., Muñoz M. E. ( 2009 ). Chemical and rheological properties of the beta-glucan produced by Pediococcus parvulus 2.6. . J Agric Food Chem 57, 18271834. [View Article] [PubMed]
    [Google Scholar]
  39. Weiss N., Schillinger U., Kandler O. ( 1983 ). Lactobacillus lactis, Lactobacillus leichmannii and Lactobacillus bulgaricus, subjective synonyms of Lactobacillus delbrueckii, and description of Lactobacillus delbrueckii subsp. lactis comb. nov. and Lactobacillus delbrueckii subsp. bulgaricus comb. nov.. Syst Appl Microbiol 4, 552557. [View Article] [PubMed]
    [Google Scholar]
  40. Whiting G. C., Carr J. G. ( 1957 ). Chlorogenic acid metabolism in cider fermentation. . Nature 180, 1479. [View Article] [PubMed]
    [Google Scholar]
  41. Yarza P., Ludwig W., Euzéby J., Amann R., Schleifer K. H., Glöckner F. O., Rosselló-Móra R. ( 2010 ). Update of the All-Species Living Tree Project based on 16S and 23S rRNA sequence analyses. . Syst Appl Microbiol 33, 291299. [View Article] [PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.059980-0
Loading
/content/journal/ijsem/10.1099/ijs.0.059980-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error