1887

Abstract

Strains CUPV261 and CUPV262 were isolated from ropy natural ciders of the Basque Country, Spain, in 2007. Cells are Gram-stain positive, non-spore-forming, motile rods, facultative anaerobes and catalase-negative. The strains are obligately homofermentative (final product -lactate) and produce exopolysaccharides from sucrose. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the highest similarity to both isolates corresponded to the type strain of (99.1 %), followed by (96.4 %), and (96.2 %), and for all other established species, 16S rRNA gene sequence similarities were below 96 %. The species delineation of strains CUPV261 and CUPV262 was evaluated through RAPD fingerprinting. In addition, a random partial genome pyrosequencing approach was performed on strain CUPV261 in order to compare it with the genome sequence of DSM 20605 and calculate indexes of average nucleotide identity (ANI) between them. Results permit the conclusion that strains CUPV261 and CUPV262 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CUPV261 ( = CECT 8227 = KCTC 21012).

Funding
This study was supported by the:
  • Spanish Ministry of Science and Innovation (Award CSD2007-00063, AGL2012-40084-C03 and AGL2009-12998-C03)
  • Basque Government (Award IT335-10)
  • Generalitat Valenciana (Award PROMETEO/2012/040)
  • ‘Gobierno Vasco, Dpto. Agricultura, Pesca y Alimentación’
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2014-09-01
2021-10-17
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