RT Journal Article SR Electronic(1)YR 2014 T1 Description of Lutimonashalocynthiae sp. nov., isolated from a golden sea squirt (Halocynthia aurantium), reclassification of Aestuariicola saemankumensis as Lutimonassaemankumensis comb. nov. and emended description of the genus Lutimonas JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_6 SP 1984 OP 1990 DO https://doi.org/10.1099/ijs.0.059923-0 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, non-motile, coccoid, ovoid or rod-shaped bacterial strain, designated RSS3-C1T, was isolated from a golden sea squirt (Halocynthia aurantium) collected from the East Sea, South Korea. Strain RSS3-C1T was found to grow optimally at 20–25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain RSS3-C1T clustered with the type strains of Lutimonas vermicola and Aestuariicola saemankumensis . Strain RSS3-C1T exhibited 98.8 % 16S rRNA gene sequence similarity to each type strain. Strain RSS3-C1T contained MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C17 : 0 3-OH and anteiso-C15 : 0 as the major fatty acids. The major polar lipids of strain RSS3-C1T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain RSS3-C1T was 39.2 mol%, and DNA–DNA relatedness to the type strains of and was 21±5.3 and 26±7.5 %, respectively. The differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain RSS3-C1T is separated from and . On the basis of the data presented, strain RSS3-C1T is considered to represent a novel species of the genus Lutimonas , for which the name Lutimonas halocynthiae sp. nov. is proposed. The type strain is RSS3-C1T ( = KCTC 32537T = CECT 8444T). In this study, it is also proposed that Aestuariicola saemankumensis should be reclassified as a member of the genus Lutimonas , as Lutimonas saemankumensis comb. nov. (type strain SMK-142T = KCTC 22171T = CCUG 55329T), and the description of the genus Lutimonas is emended., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.059923-0