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Abstract

A Gram-stain-negative, non-motile, coccoid, ovoid or rod-shaped bacterial strain, designated RSS3-C1, was isolated from a golden sea squirt () collected from the East Sea, South Korea. Strain RSS3-C1 was found to grow optimally at 20–25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain RSS3-C1 clustered with the type strains of and . Strain RSS3-C1 exhibited 98.8 % 16S rRNA gene sequence similarity to each type strain. Strain RSS3-C1 contained MK-6 as the predominant menaquinone and iso-C, iso-C 3-OH and anteiso-C as the major fatty acids. The major polar lipids of strain RSS3-C1 were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain RSS3-C1 was 39.2 mol%, and DNA–DNA relatedness to the type strains of and was 21±5.3 and 26±7.5 %, respectively. The differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain RSS3-C1 is separated from and . On the basis of the data presented, strain RSS3-C1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RSS3-C1 ( = KCTC 32537 = CECT 8444). In this study, it is also proposed that should be reclassified as a member of the genus , as comb. nov. (type strain SMK-142 = KCTC 22171 = CCUG 55329), and the description of the genus is emended.

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2014-06-01
2019-10-17
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