1887

Abstract

Two species ( nov. comb. and cf. ), respectively isolated from soil north-west of Riyadh, Saudi Arabia, and freshwater in Shanghai, eastern China, were investigated using standard methods. The species Kahl, 1931 is reclassified here in the genus and was characterized mainly by constantly showing 16 somatic kineties, three post-oral kineties with the middle one shortened, a contractile vacuole located subcaudally with an excretory pore near the posterior end of somatic kinety 2 and single caudal cilia. A -like organism having a subcaudally located contractile vacuole and fewer somatic kineties was designated cf. The small-subunit rRNA gene (SSU rDNA) sequences of these two species were characterized and their phylogenetic positions based on SSU rDNA sequences were revealed by means of Bayesian inference and maximum-likelihood analysis. Phylogenetic analyses confirmed as a monophyletic genus and supported its assignment to the order Loxocephalida. However, its family assignment remains unsupported.

Funding
This study was supported by the:
  • Natural Science Foundation of China (Award 31201708)
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2014-02-01
2024-04-25
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References

  1. Augustin V. H., Foissner W. ( 1992 ). Morphology and ecology of some ciliates (Protozoa: Ciliophora) from activated sludge. . Arch Protistenkd 141, 243283. [View Article]
    [Google Scholar]
  2. Elwood H. J., Olsen G. J., Sogin M. L. ( 1985 ). The small-subunit ribosomal RNA gene sequences from the hypotrichous ciliates Oxytricha nova and Stylonychia pustulata . . Mol Biol Evol 2, 399410.[PubMed]
    [Google Scholar]
  3. Fan X., Miao M., Al-Rasheid K. A. S., Song W. ( 2009 ). A new genus of marine scuticociliate (Protozoa, Ciliophora) from northern China, with a brief note on its phylogenetic position inferred from small subunit ribosomal DNA sequence data. . J Eukaryot Microbiol 56, 577582. [View Article] [PubMed]
    [Google Scholar]
  4. Fan X., Hu X., Al-Farraj S. A., Clamp J. C., Song W. ( 2011a ). Morphological description of three marine ciliates (Ciliophora, Scuticociliatia), with establishment of a new genus and two new species. . Eur J Protistol 47, 186196. [View Article] [PubMed]
    [Google Scholar]
  5. Fan X., Lin X., Al-Rasheid K. A. S., Warren A., Song W. ( 2011b ). The diversity of scuticociliates (Protozoa, Ciliophora): a report on eight marine forms found in coastal waters of China, with a description of one new species. . Acta Protozool 50, 219234.
    [Google Scholar]
  6. Fauré-Fremiet E. ( 1968 ). Les genres Dexiotricha Stokes et Loxocephalus Eberhard dans leurs relations auxomorphiques. . Protistologica (Paris) 4, 115125 (in French).
    [Google Scholar]
  7. Foissner W. ( 1987 ). Neue terrestrische und limnische Ciliaten (Protozoa, Ciliophora) aus Österreich und Deutschland. . In Sitzungsberichte der Österreichischen Akademie der Wissenschaften (Mathemathisch-naturwissenschaftliche Klasse, Abt. I), vol. 195, pp. 217286. Berlin:: Springer; (in German).
    [Google Scholar]
  8. Foissner W., Berger H., Kohmann F. ( 1994 ). Taxonomische und okologische Revision der Ciliaten des Saprobiensystems, Band III: Hymenostomata, Prostomatida, Nassulida (Informationsberichte des Bayerischen Landesamtes für Wasserwirtschaft, vol. 1/94). . Deggendorf:: Bayerisches Landesamt für Wasserwirtschaft; (in German).
    [Google Scholar]
  9. Gao F., Katz L. A., Song W. ( 2013 ). Multigene-based analyses on evolutionary phylogeny of two controversial ciliate orders: Pleuronematida and Loxocephalida (Protista, Ciliophora, Oligohymenophorea). . Mol Phylogenet Evol 68, 5563. [View Article] [PubMed]
    [Google Scholar]
  10. Gong J., Kim S. J., Kim S. Y., Min G. S., Roberts D. M., Warren A., Choi J. K. ( 2007 ). Taxonomic redescriptions of two ciliates, Protogastrostyla pulchra n. g., n. comb. and Hemigastrostyla enigmatica (Ciliophora: Spirotrichea, Stichotrichia), with phylogenetic analyses based on 18S and 28S rRNA gene sequences. . J Eukaryot Microbiol 54, 468478.[PubMed]
    [Google Scholar]
  11. Gu F., Ni B. ( 1993 ). The exploration of preparing protozoan specimens. . J Electron Microsc (Tokyo) 12, 525529.
    [Google Scholar]
  12. Jankowski A. W. ( 1964 ). Morphology and evolution of Ciliophora. IV. Sapropelebionts of the family Loxocephalidae fam. nova, their taxonomy and evolutionary history. . Acta Protozool 2, 3358.
    [Google Scholar]
  13. Jankowski A. W. ( 1980 ). Conspectus of a new system of the phylum Ciliophora. . Tr Zool Inst Leningr 94, 103121.
    [Google Scholar]
  14. Kahl A. ( 1931 ). Urtiere oder Protozoa I: Wimpertiere oder Ciliata (Infusoria). 2. Holotricha. . In Die Tierwelt Deutschlands, vol. 21, pp. 181398. Edited by Dahl F. . Jena:: Gustav Fischer; (in German).
    [Google Scholar]
  15. Li L., Song W., Warren A., Wang Y., Ma H., Hu X., Chen Z. ( 2006 ). Phylogenetic position of the marine ciliate, Cardiostomatella vermiforme (Kahl, 1928) Corliss, 1960 inferred from the complete SSrRNA gene sequence, with establishment of a new order Loxocephalida n. ord. (Ciliophora, Oligohymenophorea). . Eur J Protistol 42, 107114. [View Article] [PubMed]
    [Google Scholar]
  16. Li J., Lin X., Yi Z., Clamp J. C., Liu W., Al-Rasheid K. A. S. ( 2010 ). Molecules or morphogenesis: how to determine the phylogenetic assignment of Paratetrahymena (Protista, Ciliophora, Oligohymenophorea). ? Zool Scr 39, 499510. [View Article]
    [Google Scholar]
  17. Lobban C. S., Raymundo L. M., Montagnes D. J. S. ( 2011 ). Porpostoma guamensis n. sp., a philasterine scuticociliate associated with brown-band disease of corals. . J Eukaryot Microbiol 58, 103113. [View Article] [PubMed]
    [Google Scholar]
  18. Long H., Song W., Warren A., Al-Rasheid K., Gong J., Chen X. ( 2007 ). Two new ciliates from the north China seas, Schizocalyptra aeschtae nov. spec. and Sathrophilus holtae nov. spec., with new definition of the genus Sathrophilus (Ciliophora, Oligohymenophora). . Acta Protozool 46, 229245.
    [Google Scholar]
  19. Lynn D. H. ( 2008 ). In The Ciliated Protozoa, Characterization, Classification, and Guide to the Literature, , 3rd edn., pp. 414416. Dordrecht:: Springer;.
    [Google Scholar]
  20. Medlin L., Elwood H. J., Stickel S., Sogin M. L. ( 1988 ). The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. . Gene 71, 491499. [View Article] [PubMed]
    [Google Scholar]
  21. Miller M. A., Pfeiffer W., Schwartz T. ( 2010 ). Creating the CIPRES Science Gateway for inference of large phylogenetic trees. . In Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, LA, USA, 14 November 2010, pp. 18. [CrossRef]
    [Google Scholar]
  22. Nylander J. A. A. ( 2004 ). MrModeltest version 2. . Uppsala:: Evolutionary Biology Centre, Uppsala University;.
    [Google Scholar]
  23. Pan X., Shao C., Ma H., Fan X., Al-Rasheid K. A. S., Al-Farraj S. A., Hu X. ( 2011 ). Redescriptions of two marine scuticociliates from China, with notes on stomatogenesis in Parauronema longum (Ciliophora, Scuticociliatida). . Acta Protozool 50, 301310.
    [Google Scholar]
  24. Pan X., Zhu M., Ma H., Al-Rasheid K. A. S., Hu X. ( 2013 ). Morphology and small-subunit rRNA gene sequences of two novel marine ciliates, Metanophrys orientalis spec. nov. and Uronemella sinensis spec. nov. (Protista, Ciliophora, Scuticociliatia), with an improved diagnosis of the genus Uronemella . . Int J Syst Evol Microbiol 63, 35153523. [View Article] [PubMed]
    [Google Scholar]
  25. Peck R. K. ( 1974 ). Morphology and morphogenesis of Pseudomicrothorax, Glaucoma and Dexiotricha with emphasis on the types of stomatogenesis in holotrichous ciliates. . Protistologica (Paris) 10, 333370.
    [Google Scholar]
  26. Penn O., Privman E., Ashkenazy H., Landan G., Graur D., Pupko T. ( 2010a ). guidance: a web server for assessing alignment confidence scores. . Nucleic Acids Res 38 (Web Server), W23W28. [View Article] [PubMed]
    [Google Scholar]
  27. Penn O., Privman E., Landan G., Graur D., Pupko T. ( 2010b ). An alignment confidence score capturing robustness to guide tree uncertainty. . Mol Biol Evol 27, 17591767. [View Article] [PubMed]
    [Google Scholar]
  28. Posada D., Crandall K. A. ( 1998 ). modeltest: testing the model of DNA substitution. . Bioinformatics 14, 817818. [View Article] [PubMed]
    [Google Scholar]
  29. Ronquist F., Huelsenbeck J. P. ( 2003 ). MrBayes 3: Bayesian phylogenetic inference under mixed models. . Bioinformatics 19, 15721574. [View Article] [PubMed]
    [Google Scholar]
  30. Small E. B., Lynn D. H. ( 1985 ). Phylum Ciliophora Doflein, 1901. . In An Illustrated Guide to the Protozoa, pp. 393575. Edited by Lee J. J., Hutner S. H., Bovee E. C. . Lawrence, KS:: Society of Protozoologists;.
    [Google Scholar]
  31. Song W., Warren A., Roberts D., Ma H., Shang H., Wilbert N., Miao M., Yi Z. ( 2005 ). Re-evaluation of the phylogenetic position of the genus Dexiotrichides (Protozoa, Ciliophora, Scuticociliatida) inferred from stomatogenetic and molecular information for Dexiotrichides pangi . . Prog Nat Sci 15, 700707. [View Article]
    [Google Scholar]
  32. Stamatakis A., Hoover P., Rougemont J. ( 2008 ). A rapid bootstrap algorithm for the RAxML Web servers. . Syst Biol 57, 758771. [View Article] [PubMed]
    [Google Scholar]
  33. Wilbert N. ( 1975 ). Eine verbesserte Technik der Protargolimprägnation für Ciliaten. . Mikrokosmos 64, 171179 (in German)
    [Google Scholar]
  34. Wilbert N. ( 1986 ). Ciliaten aus dem interstitial des Ontario Sees. . Acta Protozool 25, 379396 (in German).
    [Google Scholar]
  35. Zhang Q., Fan X., Clamp J. C., Al-Rasheid K. A. S., Song W. ( 2010 ). Description of Paratetrahymena parawassi n. sp. using morphological and molecular evidence and a phylogenetic analysis of Paratetrahymena and other taxonomically ambiguous genera in the order Loxocephalida (Ciliophora, Oligohymenophorea). . J Eukaryot Microbiol 57, 483493. [View Article] [PubMed]
    [Google Scholar]
  36. Zhang Q., Miao M., Strüder-Kypke M. C., Al-Rasheid K. A. S., Al-Farraj S. A., Song W. ( 2011 ). Molecular evolution of Cinetochilum and Sathrophilus (Protozoa, Ciliophora, Oligohymenophorea), two genera of ciliates with morphological affinities to scuticociliates. . Zool Scr 40, 317325. [View Article]
    [Google Scholar]
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