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Abstract
A Gram-stain-positive, strictly aerobic, rod-shaped, non-motile bacterium, designated strain XH274T, was isolated from a deep sub-seafloor sediment sample collected from the South Pacific Gyre (41° 58′ S 163° 11′ W) during the Integrated Ocean Drilling Program (IODP) Expedition 329. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XH274T belonged to the genus Nocardioides and shared the highest 16S rRNA gene sequence similarity with Nocardioides kribbensis KSL-2T (96.81 %), Nocardioides daedukensis MDN22T (96.74 %) and Nocardioides psychrotolerans RHLT2-1T (96.61 %). The DNA G+C content of strain XH274T was 74.6 mol%. The cell wall of strain XH274T contained ll-2,6-diaminopimelic acid as the diagnostic diamino acid, and ribose, glucose, galactose and mannose as the major whole-cell sugars. The major fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c. The major respiratory quinone was menaquinone-8(H4). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unknown phospholipids. On the basis of data from the polyphasic analysis, strain XH274T is considered to represent a novel species in the genus Nocardioides , for which the name Nocardioides pacificus sp. nov. is proposed. The type strain is XH274T ( = DSM 27278T = JCM 19260T).
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Funding
- National Natural Science Foundation of China (Award 41276141)
- National High Technology Research & Development Program of China (Award 2012AA091605)
- China Ocean Mineral Resources Research & Development Association (Award DY125-15-R-03)