1887

Abstract

Strains YIM 77562 and YIM 77580, two novel Gram-staining-positive, filamentous bacterial isolates, were recovered from the Rehai geothermal field, Tengchong, Yunnan province, south-west China. Good growth was observed at 50–55 °C and pH 7.0. Aerial mycelium was absent on all media tested. Substrate mycelium was well-developed, long and moderately flexuous, and formed abundant, single, warty, ornamented endospores. Phylogenetic analysis of the 16S rRNA gene sequences of the two strains indicated that they belong to the family . Similarity levels between the 16S rRNA gene sequences of the two strains and those of type strains of members of the were 88.33–93.24 %; the highest sequence similarity was with DSM 45707. In both strains, the predominant menaquinone was MK-7, the diagnostic diamino acid was -diaminopimelic acid and the major cellular fatty acids were iso-C, iso-C and iso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, unidentified polar lipids and unidentified phospholipids. The genomic DNA G+C contents of strains YIM 77562 and YIM 77580 were 45.5 and 44.2 mol%, respectively. DNA–DNA relatedness data suggest that the two isolates represent a single species. Based on phylogenetic analyses and physiological and biochemical characteristics, it is proposed that the two strains represent a single novel species in a new genus, gen. nov., sp. nov. The type strain of is YIM 77562 ( = CCTCC AA 2011022 = JCM 18057).

Funding
This study was supported by the:
  • Key Project of International Cooperation of Ministry of Science & Technology (MOST) (Award 2013DFA31980)
  • National Science Foundation (Award OISE-0968421)
  • Key Project of Yunnan Provincial Natural Science Foundation (Award 2013FA004)
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2014-09-01
2021-04-10
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