%0 Journal Article %A Hahnke, Sarah %A Striesow, Jutta %A Elvert, Marcus %A Mollar, Xavier Prieto %A Klocke, Michael %T Clostridium bornimense sp. nov., isolated from a mesophilic, two-phase, laboratory-scale biogas reactor %D 2014 %J International Journal of Systematic and Evolutionary Microbiology, %V 64 %N Pt_8 %P 2792-2797 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.059691-0 %I Microbiology Society, %X A novel anaerobic, mesophilic, hydrogen-producing bacterium, designated strain M2/40T, was isolated from a mesophilic, two-phase, laboratory-scale biogas reactor fed continuously with maize silage supplemented with 5 % wheat straw. 16S rRNA gene sequence comparison revealed an affiliation to the genus Clostridium sensu stricto (cluster I of the clostridia), with Clostridium cellulovorans as the closest characterized species, showing 93.8 % sequence similarity to the type strain. Cells of strain M2/40T were rods to elongated filamentous rods that showed variable Gram staining. Optimal growth occurred at 35 °C and at pH 7. Grown on glucose, the main fermentation products were H2, CO2, formate, lactate and propionate. The DNA G+C content was 29.6 mol%. The major fatty acids (>10 %) were C16 : 0, summed feature 10 (C18 : 1ω11c/ω9t/ω6t and/or unknown ECL 17.834) and C18 : 1ω11c dimethylacetal. Based on phenotypic, chemotaxonomic and phylogenetic differences, strain M2/40T represents a novel species within the genus Clostridium , for which we propose the name Clostridium bornimense sp. nov. The type strain is M2/40T ( = DSM 25664T = CECT 8097T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.059691-0