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Abstract

A novel anaerobic, mesophilic, hydrogen-producing bacterium, designated strain M2/40, was isolated from a mesophilic, two-phase, laboratory-scale biogas reactor fed continuously with maize silage supplemented with 5 % wheat straw. 16S rRNA gene sequence comparison revealed an affiliation to the genus (cluster I of the clostridia), with as the closest characterized species, showing 93.8 % sequence similarity to the type strain. Cells of strain M2/40 were rods to elongated filamentous rods that showed variable Gram staining. Optimal growth occurred at 35 °C and at pH 7. Grown on glucose, the main fermentation products were H, CO, formate, lactate and propionate. The DNA G+C content was 29.6 mol%. The major fatty acids (>10 %) were C, summed feature 10 (Cω11/ω9/ω6 and/or unknown ECL 17.834) and Cω11 dimethylacetal. Based on phenotypic, chemotaxonomic and phylogenetic differences, strain M2/40 represents a novel species within the genus , for which we propose the name sp. nov. The type strain is M2/40 ( = DSM 25664 = CECT 8097).

Funding
This study was supported by the:
  • German Federal Ministry of Food, Agriculture and Consumer Protection (BMELV) (Award 22017111 and 22020308)
  • Deutsche Forschungsgemeinschaft (DFG)
  • Research Center/Cluster of Excellence ‘The Ocean in the Earth System’
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2014-08-01
2021-10-22
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