1887

Abstract

A novel anaerobic, mesophilic, hydrogen-producing bacterium, designated strain M2/40, was isolated from a mesophilic, two-phase, laboratory-scale biogas reactor fed continuously with maize silage supplemented with 5 % wheat straw. 16S rRNA gene sequence comparison revealed an affiliation to the genus (cluster I of the clostridia), with as the closest characterized species, showing 93.8 % sequence similarity to the type strain. Cells of strain M2/40 were rods to elongated filamentous rods that showed variable Gram staining. Optimal growth occurred at 35 °C and at pH 7. Grown on glucose, the main fermentation products were H, CO, formate, lactate and propionate. The DNA G+C content was 29.6 mol%. The major fatty acids (>10 %) were C, summed feature 10 (Cω11/ω9/ω6 and/or unknown ECL 17.834) and Cω11 dimethylacetal. Based on phenotypic, chemotaxonomic and phylogenetic differences, strain M2/40 represents a novel species within the genus , for which we propose the name sp. nov. The type strain is M2/40 ( = DSM 25664 = CECT 8097).

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2014-08-01
2020-01-28
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References

  1. Andreesen J. R., Bahl H., Gottschalk G.. ( 1989;). Introduction to the physiology and biochemistry of the genus Clostridium. . In Clostridia (Biotechnology Handbooks, vol. 3), pp. 27–62. Edited by Minton N. P., Clarke D. J... New York:: Plenum Press;. [CrossRef]
    [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M.. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef][PubMed]
    [Google Scholar]
  3. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E.. ( 1994;). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. . Int J Syst Bacteriol 44:, 812–826. [CrossRef][PubMed]
    [Google Scholar]
  4. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  5. Krause L., Diaz N. N., Edwards R. A., Gartemann K. H., Krömeke H., Neuweger H., Pühler A., Runte K. J., Schlüter A.. & other authors ( 2008;). Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor. . J Biotechnol 136:, 91–101. [CrossRef][PubMed]
    [Google Scholar]
  6. Kröber M., Bekel T., Diaz N. N., Goesmann A., Jaenicke S., Krause L., Miller D., Runte K. J., Viehöver P.. & other authors ( 2009;). Phylogenetic characterization of a biogas plant microbial community integrating clone library 16S-rDNA sequences and metagenome sequence data obtained by 454-pyrosequencing. . J Biotechnol 142:, 38–49. [CrossRef][PubMed]
    [Google Scholar]
  7. Lee Y.-J., Romanek C. S., Wiegel J.. ( 2007;). Clostridium aciditolerans sp. nov., an acid-tolerant spore-forming anaerobic bacterium from constructed wetland sediment. . Int J Syst Evol Microbiol 57:, 311–315. [CrossRef][PubMed]
    [Google Scholar]
  8. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S.. & other authors ( 2004;). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef][PubMed]
    [Google Scholar]
  9. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  10. Mumme J., Linke B., Tölle R.. ( 2010;). Novel upflow anaerobic solid-state (UASS) reactor. . Bioresour Technol 101:, 592–599. [CrossRef][PubMed]
    [Google Scholar]
  11. Rademacher A., Zakrzewski M., Schlüter A., Schönberg M., Szczepanowski R., Goesmann A., Pühler A., Klocke M.. ( 2012;). Characterization of microbial biofilms in a thermophilic biogas system by high-throughput metagenome sequencing. . FEMS Microbiol Ecol 79:, 785–799. [CrossRef][PubMed]
    [Google Scholar]
  12. Rainey F. A., Hollen B. J., Small A.. ( 2009;). Genus I. Clostridium Prazmowski 1880, 23AL. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 738–828. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  13. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  14. Schlüter A., Bekel T., Diaz N. N., Dondrup M., Eichenlaub R., Gartemann K. H., Krahn I., Krause L., Krömeke H.. & other authors ( 2008;). The metagenome of a biogas-producing microbial community of a production-scale biogas plant fermenter analysed by the 454-pyrosequencing technology. . J Biotechnol 136:, 77–90. [CrossRef][PubMed]
    [Google Scholar]
  15. Sleat R., Mah R. A., Robinson R.. ( 1984;). Isolation and characterization of an anaerobic, cellulolytic bacterium, Clostridium cellulovorans sp. nov.. Appl Environ Microbiol 48:, 88–93.[PubMed]
    [Google Scholar]
  16. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  17. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  18. Wang H., Lehtomäki A., Tolvanen K., Puhakka J., Rintala J.. ( 2009;). Impact of crop species on bacterial community structure during anaerobic co-digestion of crops and cow manure. . Bioresour Technol 100:, 2311–2315. [CrossRef][PubMed]
    [Google Scholar]
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