1887

Abstract

A novel bacterial strain, NH131, was isolated from deep-sea sediment of South China Sea. Cells were strictly aerobic, Gram-stain negative, short rod-shaped and motile with a single lateral flagellum. Strain NH131 grew optimally at pH 6.5–7.0 and 25–30 °C. 16S rRNA gene sequence analysis revealed that strain NH131 belonged to the genus , sharing the highest sequence similarity with the type strain, BD-c194 (96.2 %). The predominant fatty acids were Cω7, 11-methyl Cω7, C and C. Ubiquinone 10 was the predominant ubiquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, three glycolipids and two unknown lipids. The DNA G+C content of strain NH131 was 63.0 mol%. On the basis of the results of polyphasic identification, it is suggested that strain NH131 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is NH131 ( = JCM 19305 = KCTC 32437).

Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2014CB441503)
  • Key Laboratory of Marine Ecosystem and Biogeochemistry (LMEB), SOA (Award LMEB 201006)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.059626-0
2014-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/8/2637.html?itemId=/content/journal/ijsem/10.1099/ijs.0.059626-0&mimeType=html&fmt=ahah

References

  1. Baumann P., Baumann R. H., Schubert W. ( 1984 ). Vibrionaceae . . In Bergey’s Manual of Systematic Bacteriology, vol. 1, pp. 516550. Edited by Krieg N. R., Holt J. G. . Baltimore, MD:: Williams & Wilkins;.
    [Google Scholar]
  2. Bautista V. V., Monsalud R. G., Yokota A. ( 2010 ). Devosia yakushimensis sp. nov., isolated from root nodules of Pueraria lobata (Willd.) Ohwi. . Int J Syst Evol Microbiol 60, 627632. [View Article] [PubMed]
    [Google Scholar]
  3. Dua A., Malhotra J., Saxena A., Khan F., Lal R. ( 2013 ). Devosia lucknowensis sp. nov., a bacterium isolated from hexachlorocyclohexane (HCH) contaminated pond soil. . J Microbiol 51, 689694. [View Article] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  5. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  6. Foster J. W. ( 1944 ). Microbiological aspects of riboflavin. I. Introduction. II. Bacterial oxidation of riboflavin to lumochrome. . J Bacteriol 47, 2741.[PubMed]
    [Google Scholar]
  7. Gomori G. ( 1955 ). Preparation of buffers for use in enzyme studies. . Methods Enzymol 1, 138146. [View Article]
    [Google Scholar]
  8. Kates M. ( 1986 ). Techniques of Lipidology, , 2nd edn.. Amsterdam:: Elsevier;.
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  10. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  11. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  12. Kumar M., Verma M., Lal R. ( 2008 ). Devosia chinhatensis sp. nov., isolated from a hexachlorocyclohexane (HCH) dump site in India. . Int J Syst Evol Microbiol 58, 861865. [View Article] [PubMed]
    [Google Scholar]
  13. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  14. Lee S. D. ( 2007 ). Devosia subaequoris sp. nov., isolated from beach sediment. . Int J Syst Evol Microbiol 57, 22122215. [View Article] [PubMed]
    [Google Scholar]
  15. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 11831184.[PubMed]
    [Google Scholar]
  16. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  17. Mesbah M., Whitman W. B. ( 1989 ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479, 297306. [View Article] [PubMed]
    [Google Scholar]
  18. Nakagawa Y., Sakane T., Yokota A. ( 1996 ). Transfer of ‘Pseudomonas riboflavina’ (Foster 1944), a gram-negative, motile rod with long-chain 3-hydroxy fatty acids, to Devosia riboflavina gen. nov., sp. nov., nom. rev.. Int J Syst Bacteriol 46, 1622. [View Article] [PubMed]
    [Google Scholar]
  19. Parte A. C. ( 2014 ). LPSN–list of prokaryotic names with standing in nomenclature. . Nucleic Acids Res 42 (D1), D613D616. [View Article] [PubMed]
    [Google Scholar]
  20. Rivas R., Willems A., Subba-Rao N. S., Mateos P. F., Dazzo F. B., Kroppenstedt R. M., Martínez-Molina E., Gillis M., Velázquez E. ( 2003 ). Description of Devosia neptuniae sp. nov. that nodulates and fixes nitrogen in symbiosis with Neptunia natans, an aquatic legume from India. . Syst Appl Microbiol 26, 4753. [View Article] [PubMed]
    [Google Scholar]
  21. Romanenko L. A., Tanaka N., Svetashev V. I. ( 2013 ). Devosia submarina sp. nov., isolated from deep-sea surface sediments. . Int J Syst Evol Microbiol 63, 30793085. [View Article] [PubMed]
    [Google Scholar]
  22. Ryu S. H., Chung B. S., Le N. T., Jang H. H., Yun P.-Y., Park W., Jeon C. O. ( 2008 ). Devosia geojensis sp. nov., isolated from diesel-contaminated soil in Korea. . Int J Syst Evol Microbiol 58, 633636. [View Article] [PubMed]
    [Google Scholar]
  23. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  24. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  27. Vanparys B., Heylen K., Lebbe L., De Vos P. ( 2005 ). Devosia limi sp. nov., isolated from a nitrifying inoculum. . Int J Syst Evol Microbiol 55, 19972000. [View Article] [PubMed]
    [Google Scholar]
  28. Verma M., Kumar M., Dadhwal M., Kaur J., Lal R. ( 2009 ). Devosia albogilva sp. nov. and Devosia crocina sp. nov., isolated from a hexachlorocyclohexane dump site. . Int J Syst Evol Microbiol 59, 795799. [View Article] [PubMed]
    [Google Scholar]
  29. Xu X.-W., Wu Y.-H., Zhou Z., Wang C.-S., Zhou Y.-G., Zhang H.-B., Wang Y., Wu M. ( 2007 ). Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. . Int J Syst Evol Microbiol 57, 16191624. [View Article] [PubMed]
    [Google Scholar]
  30. Yoo S.-H., Weon H. Y., Kim B. Y., Hong S.-B., Kwon S.-W., Cho Y.-H., Go S.-J., Stackebrandt E. ( 2006 ). Devosia soli sp. nov., isolated from greenhouse soil in Korea. . Int J Syst Evol Microbiol 56, 26892692. [View Article] [PubMed]
    [Google Scholar]
  31. Yoon J.-H., Kang S.-J., Park S., Oh T.-K. ( 2007 ). Devosia insulae sp. nov., isolated from soil, and emended description of the genus Devosia . . Int J Syst Evol Microbiol 57, 13101314. [View Article] [PubMed]
    [Google Scholar]
  32. Zhang D. C., Redzic M., Liu H. C., Zhou Y. G., Schinner F., Margesin R. ( 2012 ). Devosia psychrophila sp. nov. and Devosia glacialis sp. nov., from alpine glacier cryoconite, and an emended description of the genus Devosia . . Int J Syst Evol Microbiol 62, 710715. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.059626-0
Loading
/content/journal/ijsem/10.1099/ijs.0.059626-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error