A Gram-negative bacterium, strain CZ-AM5T, was isolated from an aerated biological filter in a marine recirculating aquaculture system in Tianjin, China. Its taxonomic position was investigated by using a polyphasic approach. Cells of strain CZ-AM5T were non-spore-forming rods, 0.5–0.8 µm wide and 1.2–2.0 µm long, and motile by means of one or two polar or lateral flagella. Strain CZ-AM5T was strictly aerobic, heterotrophic, oxidase-negative and catalase-positive. Growth occurred at 15–40 °C (optimum, 30–35 °C), at pH 6.5–10.5 (optimum, pH 7.0–7.5) and in the presence of 0–12.0 % (w/v) NaCl (optimum, 4.0 %). The predominant fatty acid was C18 : 1ω7c (80.3 %). Ubiquinone 10 (Q-10) was the sole respiratory quinone. The polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unknown aminolipid, an unknown phospholipid and three unknown lipids. The DNA G+C content was 60.4 mol%. Strain CZ-AM5T showed the highest 16S rRNA gene sequence similarity (96.5 %) to Phaeobacter caeruleus LMG 24369T; it exhibited 16S rRNA gene sequence similarity of 95.0–96.5, 95.2–96.3, 96.2, 94.6–95.7 and 94.8–95.8 % to members of the genera Phaeobacter, Ruegeria, Citreimonas, Leisingera and Donghicola, respectively. However, phylogenetic trees based on 16S rRNA gene sequences showed that strain CZ-AM5T did not join any of the above genera, but formed a distinct lineage in the trees. On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain CZ-AM5T is considered to represent a novel genus and species of the family Rhodobacteraceae, for which the name Cribrihabitans marinus gen. nov., sp. nov. is proposed. The type strain of Cribrihabitans marinus is CZ-AM5T ( = CGMCC 1.13219T = JCM 19401T).
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Funding
This study was supported by the:
National Science and Technology Supporting Project of China
(Award 2011BAD13B04)
CAS Knowledge Innovation Project
(Award KZCX2-EW-Q212)
Public Service Sectors (Agriculture) Special Project
(Award 201003024)
ChoiD. H.,
ChoB. C.(2006).Citreimonas salinaria gen. nov., sp. nov., a member of the Roseobacter clade isolated from a solar saltern. . Int J Syst Evol Microbiol56, 2799–2803. [View Article][PubMed]
CollinsM. D.(1985). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by
GoodfellowM.,
MinnikinD. E.
. London:: Academic Press;.
GarrityG. M.,
BellJ. A.,
LilburnT.(2005). Order III. Rhodobacterales ord. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2C, p. 161. Edited by
BrennerD. J.,
KriegN. R.,
StaleyJ. T.,
GarrityG. M.
. New York:: Springer;.
GiebelH. A.,
KalhoeferD.,
Gahl-JanssenR.,
ChooY. J.,
LeeK.,
ChoJ. C.,
TindallB. J.,
RhielE.,
BeardsleyC.& other authors (2013).Planktomarina temperata gen. nov., sp. nov., belonging to the globally distributed RCA cluster of the marine Roseobacter clade, isolated from the German Wadden Sea. . Int J Syst Evol Microbiol63, 4207–4217. [View Article][PubMed]
GunstoneF. D.,
JacobsbergF. R.(1972). Fatty acids, part 35: the preparation and properties of a complete series of methyl epoxyoctadecanoates. . Chem Phys Lipids9, 26–34. [View Article]
KimK. K.,
LeeJ.-S.,
LeeK. C.,
OhH.-M.,
KimS.-G.(2010).Pontibaca methylaminivorans gen. nov., sp. nov., a member of the family Rhodobacteraceae
. . Int J Syst Evol Microbiol60, 2170–2175. [View Article][PubMed]
KimuraM.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16, 111–120. [View Article][PubMed]
LaiP. Y.,
MiaoL.,
LeeO. O.,
LiuL. L.,
ZhouX. J.,
XuY.,
Al-SuwailemA.,
QianP. Y.(2013).Profundibacterium mesophilum gen. nov., sp. nov., a novel member in the family Rhodobacteraceae isolated from deep-sea sediment in the Red Sea, Saudi Arabia. . Int J Syst Evol Microbiol63, 1007–1012. [View Article][PubMed]
LeeD. H.,
ChoS. J.,
KimS. M.,
LeeS. B.(2013).Sagittula marina sp. nov., isolated from seawater and emended description of the genus Sagittula
. . Int J Syst Evol Microbiol63, 2101–2107. [View Article][PubMed]
MarmurJ.,
DotyP.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol5, 109–118. [View Article][PubMed]
MartensT.,
HeidornT.,
PukallR.,
SimonM.,
TindallB. J.,
BrinkhoffT.(2006). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera
. . Int J Syst Evol Microbiol56, 1293–1304. [View Article][PubMed]
MuramatsuY.,
UchinoY.,
KasaiH.,
SuzukiK.,
NakagawaY.(2007).Ruegeria mobilis sp. nov., a member of the Alphaproteobacteria isolated in Japan and Palau. . Int J Syst Evol Microbiol57, 1304–1309. [View Article][PubMed]
PetursdottirS. K.,
KristjanssonJ. K.(1997).Silicibacter lacuscaerulensis gen. nov., sp. nov., a mesophilic moderately halophilic bacterium characteristic of the Blue Lagoon geothermal lake in Iceland. . Extremophiles1, 94–99. [View Article][PubMed]
Ruiz-PonteC.,
CiliaV.,
LambertC.,
NicolasJ. L.(1998).Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus
. . Int J Syst Bacteriol48, 537–542. [View Article][PubMed]
SchaeferJ. K.,
GoodwinK. D.,
McDonaldI. R.,
MurrellJ. C.,
OremlandR. S.(2002).Leisingera methylohalidivorans gen. nov., sp. nov., a marine methylotroph that grows on methyl bromide. . Int J Syst Evol Microbiol52, 851–859. [View Article][PubMed]
SubhashY.,
TusharL.,
SasikalaCh.,
RamanaCh. V.(2013).Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern. . Int J Syst Evol Microbiol63, 2132–2137. [View Article][PubMed]
TanT.,
WangB.,
ShaoZ.(2009).Donghicola xiamenensis sp. nov., a marine bacterium isolated from seawater of the Taiwan Strait in China. . Int J Syst Evol Microbiol59, 1143–1147. [View Article][PubMed]
VentosaA.,
MarquezM. C.,
KocurM.,
TindallB. J.(1993). Comparative study of “Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus
. . Int J Syst Bacteriol43, 245–248. [View Article][PubMed]
YinD.,
ChenL.,
AoJ.,
AiC.,
ChenX.(2013).Pleomorphobacterium xiamenense gen. nov., sp. nov., a moderate thermophile isolated from a terrestrial hot spring. . Int J Syst Evol Microbiol63, 1868–1873. [View Article][PubMed]
YoonJ.-H.,
KangS.-J.,
OhT.-K.(2007a).Donghicola eburneus gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. . Int J Syst Evol Microbiol57, 73–76. [View Article][PubMed]
YoonJ.-H.,
KangS.-J.,
LeeS.-Y.,
OhT.-K.(2007b).Phaeobacter daeponensis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol57, 856–861. [View Article][PubMed]