1887

Abstract

A Gram-stain-positive, short rod-shaped and motile, mildly halotolerant, endospore-forming bacterium, FJAT-13985, was isolated from the internal tissues of root. Strain FJAT-13985 grew at 20–45 °C (optimum 30 °C) and pH 5.7–9.0 (optimum pH 7.0) and in the presence of 0–2 % (w/v) NaCl [optimum 1 % (w/v)]. The strain was catalase-positive and oxidase-negative. The cell wall of strain FJAT-13985 contained -diaminopimelic acid and the predominant isoprenoid quinone was MK-7 (97.4 %). The major fatty acids of the strain were anteiso-C (23.3 %) and iso-C (40.8 %). The DNA G+C content was 41.64 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FJAT-13985 is a member of the genus and is most closely related to DSM 15600 (98.4 %), DSM 15602 (98.2 %) and DSM 15603 (98.3 %). DNA–DNA hybridization indicated that relatedness between strain FJAT-13985 and its closest relative, DSM 15600, was 36.63 %. The phenotypic, chemotaxonomic and genotypic properties clearly indicate that strain FJAT-13985 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FJAT-13985 ( = DSM 25968 = CGMCC1.12238).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China (NSFC) , (Award 31370059)
  • , Chinese Ministry of Agriculture , (Award 948 project (2011-G25))
  • , Project of Agriculture Science and Technology achievement transformation , (Award 2010GB2C400220)
  • , Chinese Ministry of Science and Technology , (Award 2012DFA31120)
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2014-10-01
2020-09-21
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