1887

Abstract

A Gram-stain-positive, short rod-shaped and motile, mildly halotolerant, endospore-forming bacterium, FJAT-13985, was isolated from the internal tissues of root. Strain FJAT-13985 grew at 20–45 °C (optimum 30 °C) and pH 5.7–9.0 (optimum pH 7.0) and in the presence of 0–2 % (w/v) NaCl [optimum 1 % (w/v)]. The strain was catalase-positive and oxidase-negative. The cell wall of strain FJAT-13985 contained -diaminopimelic acid and the predominant isoprenoid quinone was MK-7 (97.4 %). The major fatty acids of the strain were anteiso-C (23.3 %) and iso-C (40.8 %). The DNA G+C content was 41.64 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FJAT-13985 is a member of the genus and is most closely related to DSM 15600 (98.4 %), DSM 15602 (98.2 %) and DSM 15603 (98.3 %). DNA–DNA hybridization indicated that relatedness between strain FJAT-13985 and its closest relative, DSM 15600, was 36.63 %. The phenotypic, chemotaxonomic and genotypic properties clearly indicate that strain FJAT-13985 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FJAT-13985 ( = DSM 25968 = CGMCC1.12238).

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2014-10-01
2019-11-13
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References

  1. Albuquerque L., Tiago I., Taborda M., Nobre M. F., Veríssimo A., da Costa M. S.. ( 2008;). Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond. . Int J Syst Evol Microbiol 58:, 226–230. [CrossRef][PubMed]
    [Google Scholar]
  2. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D.. ( 1991;). Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. . Lett Appl Microbiol 13:, 202–206. [CrossRef]
    [Google Scholar]
  3. Atlas R. M.. ( 1993;). Handbook of Microbiological Media. Edited by Parks L. C... Boca Raton, FL:: CRC Press;.
    [Google Scholar]
  4. Bibi F., Chung E. J., Jeon C. O., Chung Y. R.. ( 2011;). Bacillus graminis sp. nov., an endophyte isolated from a coastal dune plant. . Int J Syst Evol Microbiol 61:, 1567–1571. [CrossRef][PubMed]
    [Google Scholar]
  5. Chen Y. G., Cui X. L., Pukall R., Li H. M., Yang Y. L., Xu L. H., Wen M. L., Peng Q., Jiang C. L.. ( 2007;). Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. . Int J Syst Evol Microbiol 57:, 2327–2332. [CrossRef][PubMed]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  7. Dong X., Cai M.. ( 2001;). Manual of systematic and determinative bacteriology. . In: Science Press, Beijing. (In Chinese). [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  9. Goodfellow M., Stainsby F. M., Davenport R., Chun J., Curtis T.. ( 1998;). Activated sludge foaming: the true extent of actinomycete diversity. . Water Sci Technol 37:, 511–519. [CrossRef]
    [Google Scholar]
  10. Gregersen T.. ( 1978;). Rapid method for distinction of Gram-negative from Gram-positive bacteria. . Eur J Appl Microbiol Biotechnol 5:, 123–127. [CrossRef]
    [Google Scholar]
  11. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A.. ( 1996;). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46:, 234–239. [CrossRef][PubMed]
    [Google Scholar]
  12. Hasegawa T., Takizawa M., Tanida S.. ( 1983;). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  13. Heyrman J., Vanparys B., Logan N. A., Balcaen A., Rodríguez-Díaz M., Felske A., De Vos P.. ( 2004;). Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands. . Int J Syst Evol Microbiol 54:, 47–57. [CrossRef][PubMed]
    [Google Scholar]
  14. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H.. (editors) ( 1985;). Genetic Manipulation of Streptomyces. A Laboratory Manual. Norwich:: John Innes Foundation;.
    [Google Scholar]
  15. Huss V. A. R., Festl H., Schleifer K. H.. ( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef][PubMed]
    [Google Scholar]
  16. Jahnke K. D.. ( 1992;). basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. . J Microbiol Methods 15:, 61–73. [CrossRef]
    [Google Scholar]
  17. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N... New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  18. Kämpfer P.. ( 1994;). Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. . Syst Appl Microbiol 17:, 86–98. [CrossRef]
    [Google Scholar]
  19. Kämpfer P., Kroppenstedt R. M.. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  20. Kanso S., Greene A. C., Patel B. K.. ( 2002;). Bacillus subterraneus sp. nov., an iron- and manganese-reducing bacterium from a deep subsurface Australian thermal aquifer. . Int J Syst Evol Microbiol 52:, 869–874. [CrossRef][PubMed]
    [Google Scholar]
  21. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  22. Ko K. S., Oh W. S., Lee M. Y., Lee J. H., Lee H., Peck K. R., Lee N. Y., Song J.-H.. ( 2006;). Bacillus infantis sp. nov. and Bacillus idriensis sp. nov., isolated from a patient with neonatal sepsis. . Int J Syst Evol Microbiol 56:, 2541–2544. [CrossRef][PubMed]
    [Google Scholar]
  23. Logan N. A., De Vos P.. ( 2009;). Genus I. Bacillus. Cohn 1872, 174AL. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 21–128. Edited by De Vos P., Garrity G. M, Jones D., Krieg N. R., Ludwig W., Rainey F. A, Schleifer K.-H, Whitman W. B.. New York:: Springer;.
    [Google Scholar]
  24. Logan N. A., Lebbe L., Hoste B., Goris J., Forsyth G., Heyndrickx M., Murray B. L., Syme N., Wynn-Williams D. D., De Vos P.. ( 2000;). Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov.. Int J Syst Evol Microbiol 50:, 1741–1753.[PubMed]
    [Google Scholar]
  25. Logan N. A., Lebbe L., Verhelst A., Goris J., Forsyth G., Rodríguez-Díaz M., Heyndrickx M., De Vos P.. ( 2004;). Bacillus shackletonii sp. nov., from volcanic soil on Candlemas Island, South Sandwich archipelago. . Int J Syst Evol Microbiol 54:, 373–376. [CrossRef][PubMed]
    [Google Scholar]
  26. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef][PubMed]
    [Google Scholar]
  27. Priest F. G., Goodfellow M., Todd C.. ( 1988;). A numerical classification of the genus Bacillus. . J Gen Microbiol 134:, 1847–1882.[PubMed]
    [Google Scholar]
  28. Reva O. N., Smirnov V. V., Pettersson B., Priest F. G.. ( 2002;). Bacillus endophyticus sp. nov., isolated from the inner tissues of cotton plants (Gossypium sp.). . Int J Syst Evol Microbiol 52:, 101–107.[PubMed]
    [Google Scholar]
  29. Rössler D., Ludwig W., Schleifer K. H., Lin C., McGill T. J., Wisotzkey J. D., Jurtshuk P. Jr, Fox G. E.. ( 1991;). Phylogenetic diversity in the genus Bacillus as seen by 16S rRNA sequencing studies. . Syst Appl Microbiol 14:, 266–269. [CrossRef][PubMed]
    [Google Scholar]
  30. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  31. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC News 20:, 16.
    [Google Scholar]
  32. Seiler H., Schmidt V., Wenning M., Scherer S.. ( 2012;). Bacillus kochii sp. nov., isolated from foods and a pharmaceuticals manufacturing site. . Int J Syst Evol Microbiol 62:, 1092–1097. [CrossRef][PubMed]
    [Google Scholar]
  33. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  34. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  35. Stackebrandt E., Frederiksen W., Garrity G. M., Grimont P. A. D., Kämpfer P., Maiden M. C. J., Nesme X., Rosselló-Mora R., Swings J.. & other authors ( 2002;). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52:, 1043–1047. [CrossRef][PubMed]
    [Google Scholar]
  36. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  37. Ten L. N., Baek S. H., Im W. T., Larina L. L., Lee J. S., Oh H. M., Lee S. T.. ( 2007;). Bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 57:, 2532–2537. [CrossRef][PubMed]
    [Google Scholar]
  38. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  39. Tiago I., Pires C., Mendes V., Morais P. V., da Costa M. S., Veríssimo A.. ( 2006;). Bacillus foraminis sp. nov., isolated from a non-saline alkaline groundwater. . Int J Syst Evol Microbiol 56:, 2571–2574. [CrossRef][PubMed]
    [Google Scholar]
  40. Vaishampayan P., Probst A., Krishnamurthi S., Ghosh S., Osman S., McDowall A., Ruckmani A., Mayilraj S., Venkateswaran K.. ( 2010;). Bacillus horneckiae sp. nov., isolated from a spacecraft-assembly clean room. . Int J Syst Evol Microbiol 60:, 1031–1037. [CrossRef][PubMed]
    [Google Scholar]
  41. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  42. Xu D., Côté J. C.. ( 2003;). Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3′ end 16S rDNA and 5′ end 16S-23S ITS nucleotide sequences. . Int J Syst Evol Microbiol 53:, 695–704. [CrossRef][PubMed]
    [Google Scholar]
  43. Yoon J. H., Kang S. S., Lee K. C., Kho Y. H., Choi S. H., Kang K. H., Park Y. H.. ( 2001;). Bacillus jeotgali sp. nov., isolated from jeotgal, Korean traditional fermented seafood. . Int J Syst Evol Microbiol 51:, 1087–1092. [CrossRef][PubMed]
    [Google Scholar]
  44. Zhang J., Wang J. W., Fang C. Y., Song F., Xin Y. H., Qu L., Ding K.. ( 2010;). Bacillus oceanisediminis sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 60:, 2924–2929. [CrossRef][PubMed]
    [Google Scholar]
  45. Zhang Y. Z., Chen W. F., Li M., Sui X. H., Liu H. C., Zhang X. X., Chen W. X.. ( 2012;). Bacillus endoradicis sp. nov., an endophytic bacterium isolated from soybean root. . Int J Syst Evol Microbiol 62:, 359–363. [CrossRef][PubMed]
    [Google Scholar]
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