1887

Abstract

A Gram-stain-negative, non-spore-forming, motile, strictly aerobic bacterial strain, designated CAU 1294, was isolated from a sand sample and its taxonomic position was investigated using a polyphasic approach. The strain grew optimally at pH 6.5 and 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1294 formed a lineage with member of the genus and exhibited similarity to MKT133 (96.3 % similarity) and LMG 23452 (95.1 % similarity). Strain CAU 1294 contained Q-10 as the predominant respiratory quinone. Cω7, C and cyclo-Cω8 were the major cellular fatty acids. The polar lipids were composed of phosphatidylethanolamine, phosphatidylmethylethanolamine and two unidentified phospholipids. The DNA G+C content was 56.6 mol%. On the basis of these results, strain CAU 1294 is considered to represent a novel species of the genus , for which the name is proposed. The type strain is CAU 1294 ( = KCTC 32476 = CECT 8513). An emended description of the genus is also proposed.

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2014-05-01
2024-04-25
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References

  1. Cappuccino J. G., Sherman N. ( 2002 ). Microbiology: a Laboratory Manual, , 6th edn.. Menlo Park, CA:: Benjamin/Cummings;.
    [Google Scholar]
  2. Cho S. L., Nam S. W., Yoon J. H., Lee J. S., Sukhoom A., Kim W. ( 2008 ). Lactococcus chungangensis sp. nov., a lactic acid bacterium isolated from activated sludge foam. . Int J Syst Evol Microbiol 58, 18441849. [View Article] [PubMed]
    [Google Scholar]
  3. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  5. Felsenstein J. ( 1989 ). phylip – phylogeny inference package (version 3.2). . Cladistics 5, 164166.
    [Google Scholar]
  6. Fitch W. M., Margoliash E. ( 1967 ). Construction of phylogenetic trees. . Science 155, 279284. [View Article] [PubMed]
    [Google Scholar]
  7. Gordon R. E., Mihm J. M. ( 1962 ). Identification of Nocardia caviae (Erikson) nov. comb.. Ann N Y Acad Sci 98, 628636. [View Article]
    [Google Scholar]
  8. Jordan E. M., Thompson F. L., Zhang X.-H., Li Y., Vancanneyt M., Kroppenstedt R. M., Priest F. G., Austin B. ( 2007 ). Sneathiella chinensis gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in Qingdao, China. . Int J Syst Evol Microbiol 57, 114121. [View Article] [PubMed]
    [Google Scholar]
  9. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. H. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  11. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161208. [View Article]
    [Google Scholar]
  12. Kurahashi M., Fukunaga Y., Harayama S., Yokota A. ( 2008 ). Sneathiella glossodoripedis sp. nov., a marine alphaproteobacterium isolated from the nudibranch Glossodoris cincta, and proposal of Sneathiellales ord. nov. and Sneathiellaceae fam. nov.. Int J Syst Evol Microbiol 58, 548552. [View Article] [PubMed]
    [Google Scholar]
  13. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  14. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 11831184.[PubMed]
    [Google Scholar]
  15. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  16. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. ( 1980 ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188, 221233. [View Article]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  20. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
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