1887

Abstract

A novel Gram-staining-positive, endospore-forming, rod-shaped, facultatively anaerobic, thermotolerant bacterium, designated strain MO-04, was isolated from a marine animal resources (MAR) compost. The 16S rRNA gene sequence of strain MO-04 showed 99.4 % similarity with R-6488, 94.1 % similarity with CNCM I-1378, 93.3 % similarity with LMG 22167, 93.2 % similarity with IFO 15566 and the similarities with other species were less than 93 %. DNA–DNA relatedness between strain MO-04 and DSM 23332 was 45 %. The DNA G+C content of strain MO-04 was 33.4 mol%, comparatively lower than that of R-6488 (35.0 mol%). Strain MO-04 grew at 35–61 °C (optimum 50 °C), pH 4.5–9.0 (optimum pH 7.2) and tolerated up to 8.0 % (w/v) NaCl (optimum 2 %). The MO-04 cell wall peptidoglycan type was -2,6-diaminopimelic acid, and the major fatty acids were C, C, C and C. The major polar lipids were represented by diphosphatidylglycerol and phosphatidylglycerol and two unidentified phospholipids. The analysed polyphasic data presented here clearly indicate that the isolate MO-04 is considered to represent a novel species within the genus for which the name sp. nov. is proposed. The type strain of is MO-04 ( = JCM 19325 = KCTC 33163).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.059329-0
2014-08-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/8/2668.html?itemId=/content/journal/ijsem/10.1099/ijs.0.059329-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M.. ( 1997;). Handbook of Microbiological Media. , 2nd edn.. Edited by Parks L. C.., New York:: CRC Press;.
    [Google Scholar]
  2. Beffa T., Blanc M., Lyon P. F., Vogt G., Marchiani M., Fischer J. L., Aragno M.. ( 1996;). Isolation of Thermus strains from hot composts (60 to 80° C). . Appl Environ Microbiol 62:, 1723–1727.[PubMed]
    [Google Scholar]
  3. Blanc M., Marilley L., Beffa T., Aragno M.. ( 1997;). Rapid identification of heterotrophic, thermophilic, spore-forming bacteria isolated from hot composts. . Int J Syst Bacteriol 47:, 1246–1248. [CrossRef][PubMed]
    [Google Scholar]
  4. Cole J. R., Wang Q., Cardenas E., Fish J., Chai B., Farris R. J., Kulam-Syed-Mohideen A. S., McGarrell D. M., Marsh T.. & other authors ( 2009;). The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. . Nucleic Acids Res 37: (Database issue), D141–D145. [CrossRef][PubMed]
    [Google Scholar]
  5. Combet-Blanc Y., Ollivier B., Streicher C., Patel B. K. C., Dwivedi P. P., Pot B., Prensier G., Garcia J. L.. ( 1995;). Bacillus thermoamylovorans sp. nov., a moderately thermophilic and amylolytic bacterium. . Int J Syst Bacteriol 45:, 9–16. [CrossRef][PubMed]
    [Google Scholar]
  6. Coorevits A., Logan N. A., Dinsdale A. E., Halket G., Scheldeman P., Heyndrickx M., Schumann P., Van Landschoot A., De Vos P.. ( 2011;). Bacillus thermolactis sp. nov., isolated from dairy farms, and emended description of Bacillus thermoamylovorans. . Int J Syst Evol Microbiol 61:, 1954–1961. [CrossRef][PubMed]
    [Google Scholar]
  7. Daron H. H.. ( 1970;). Fatty acid composition of lipid extracts of a thermophilic Bacillus species. . J Bacteriol 101:, 145–151.[PubMed]
    [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  9. Gonzalez J. M., Saiz-Jimenez C.. ( 2005;). A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures. . Extremophiles 9:, 75–79. [CrossRef][PubMed]
    [Google Scholar]
  10. Heyndrickx M., Scheldeman P., Forsyth G., Lebbe L., Rodríguez-Díaz M., Logan N. A., De Vos P.. ( 2005;). Bacillus ruris sp. nov., from dairy farms. . Int J Syst Evol Microbiol 55:, 2551–2554. [CrossRef][PubMed]
    [Google Scholar]
  11. Heyrman J., Rodríguez-Díaz M., Devos J., Felske A., Logan N. A., De Vos P.. ( 2005;). Bacillus arenosi sp. nov., Bacillus arvi sp. nov. and Bacillus humi sp. nov., isolated from soil. . Int J Syst Evol Microbiol 55:, 111–117. [CrossRef][PubMed]
    [Google Scholar]
  12. Hugh R., Leifson E.. ( 1953;). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria. . J Bacteriol 66:, 24–26.[PubMed]
    [Google Scholar]
  13. Kates M.. ( 1972;). Techniques of Lipidology. New York:: Elsevier;.
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  15. Logan N. A., de Vos P.. ( 2009;). Genus I. Bacillus. . In Bergey’s Manual of Systematic Bacteriology, vol. 3, pp. 21–128. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  16. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L.. & other authors ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59:, 2114–2121. [CrossRef][PubMed]
    [Google Scholar]
  17. Maidak B. L., Cole J. R., Lilburn T. G., Parker C. T. Jr, Saxman P. R., Farris R. J., Garrity G. M., Olsen G. J., Schmidt T. M., Tiedje J. M.. ( 2001;). The RDP-II (Ribosomal Database Project). . Nucleic Acids Res 29:, 173–174. [CrossRef][PubMed]
    [Google Scholar]
  18. Marmur J.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  20. Moreira A. P. B., Pereira N. Jr, Thompson F. L.. ( 2011;). Usefulness of a real-time PCR platform for G+C content and DNA-DNA hybridization estimations in vibrios. . Int J Syst Evol Microbiol 61:, 2379–2383. [CrossRef][PubMed]
    [Google Scholar]
  21. Nagel M., Andreesen J. R.. ( 1991;). Bacillus niacini sp. nov., a nicotinate-metabolizing mesophile isolated from soil. . Int J Syst Bacteriol 41:, 134–139. [CrossRef]
    [Google Scholar]
  22. Niisawa C., Oka S., Kodama H., Hirai M., Kumagai Y., Mori K., Matsumoto J., Miyamoto H., Miyamoto H.. ( 2008;). Microbial analysis of a composted product of marine animal resources and isolation of bacteria antagonistic to a plant pathogen from the compost. . J Gen Appl Microbiol 54:, 149–158. [CrossRef][PubMed]
    [Google Scholar]
  23. Rosselló-Mora R., Amann R.. ( 2001;). The species concept for prokaryotes. . FEMS Microbiol Rev 25:, 39–67. [CrossRef][PubMed]
    [Google Scholar]
  24. Sakai K., Yokota A., Kurokawa H., Wakayama M., Moriguchi M.. ( 1998;). Purification and characterization of three thermostable endochitinases of a noble Bacillus strain, MH-1, isolated from chitin-containing compost. . Appl Environ Microbiol 64:, 3397–3402.[PubMed]
    [Google Scholar]
  25. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  26. Schaeffer A. B., Fulton M. D.. ( 1933;). A simplified method of staining endospores. . Science 77:, 194. [CrossRef][PubMed]
    [Google Scholar]
  27. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  28. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  29. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  30. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  31. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  32. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: Molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30:, 2725–2729. [CrossRef][PubMed]
    [Google Scholar]
  33. Tashiro Y., Matsumoto H., Miyamoto H., Okugawa Y., Pramod P., Miyamoto H., Sakai K.. ( 2013;). A novel production process for optically pure l-lactic acid from kitchen refuse using a bacterial consortium at high temperatures. . Bioresour Technol 146:, 672–681. [CrossRef][PubMed]
    [Google Scholar]
  34. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  35. Tittsler R. P., Sandholzer L. A.. ( 1936;). The use of semi-solid agar for the detection of bacterial motility. . J Bacteriol 31:, 575–580.[PubMed]
    [Google Scholar]
  36. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A.. ( 1982;). Numerical taxonomy of moderately halophilic Gram-negative rods. . J Gen Microbiol 128:, 1959–1968.
    [Google Scholar]
  37. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.059329-0
Loading
/content/journal/ijsem/10.1099/ijs.0.059329-0
Loading

Data & Media loading...

Supplements

Supplementary Material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error