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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_8

Thermotolerant sp. nov. isolated from marine animal resources compost Free

Abstract

A novel Gram-staining-positive, endospore-forming, rod-shaped, facultatively anaerobic, thermotolerant bacterium, designated strain MO-04, was isolated from a marine animal resources (MAR) compost. The 16S rRNA gene sequence of strain MO-04 showed 99.4 % similarity with R-6488, 94.1 % similarity with CNCM I-1378, 93.3 % similarity with LMG 22167, 93.2 % similarity with IFO 15566 and the similarities with other species were less than 93 %. DNA–DNA relatedness between strain MO-04 and DSM 23332 was 45 %. The DNA G+C content of strain MO-04 was 33.4 mol%, comparatively lower than that of R-6488 (35.0 mol%). Strain MO-04 grew at 35–61 °C (optimum 50 °C), pH 4.5–9.0 (optimum pH 7.2) and tolerated up to 8.0 % (w/v) NaCl (optimum 2 %). The MO-04 cell wall peptidoglycan type was -2,6-diaminopimelic acid, and the major fatty acids were C, C, C and C. The major polar lipids were represented by diphosphatidylglycerol and phosphatidylglycerol and two unidentified phospholipids. The analysed polyphasic data presented here clearly indicate that the isolate MO-04 is considered to represent a novel species within the genus for which the name sp. nov. is proposed. The type strain of is MO-04 ( = JCM 19325 = KCTC 33163).

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Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award  23580116)
  • Ministry of Education, Culture, Sports, Science and Technology, Japan
© 2014 IUMS
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2014-08-01
2025-04-28
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Thermotolerant Bacillus kokeshiiformis sp. nov. isolated from marine animal resources compost
Int J Syst Evol Microbiol 64, 2668 (2014); https://doi.org/10.1099/ijs.0.059329-0
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