RT Journal Article SR Electronic(1) A1 Spitaels, Freek A1 Wieme, Anneleen A1 Balzarini, Tom A1 Cleenwerck, Ilse A1 Van Landschoot, Anita A1 De Vuyst, Luc A1 Vandamme, PeterYR 2014 T1 Gluconobacter cerevisiae sp. nov., isolated from the brewery environment JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_4 SP 1134 OP 1141 DO https://doi.org/10.1099/ijs.0.059311-0 PB Microbiology Society, SN 1466-5034, AB Three strains, LMG 27748T, LMG 27749 and LMG 27882 with identical MALDI-TOF mass spectra were isolated from samples taken from the brewery environment. Analysis of the 16S rRNA gene sequence of strain LMG 27748T revealed that the taxon it represents was closely related to type strains of the species Gluconobacter albidus (100 % sequence similarity), Gluconobacter kondonii (99.9 %), Gluconobacter sphaericus (99.9 %) and Gluconobacter kanchanaburiensis (99.5 %). DNA–DNA hybridization experiments on the type strains of these species revealed moderate DNA relatedness values (39–65 %). The three strains used d-fructose, d-sorbitol, meso-erythritol, glycerol, l-sorbose, ethanol (weakly), sucrose and raffinose as a sole carbon source for growth (weak growth on the latter two carbon sources was obtained for strains LMG 27748T and LMG 27882). The strains were unable to grow on glucose-yeast extract medium at 37 °C. They produced acid from meso-erythritol and sucrose, but not from raffinose. d-Gluconic acid, 2-keto-d-gluconic acid and 5-keto-d-gluconic acid were produced from d-glucose, but not 2,5-diketo-d-gluconic acid. These genotypic and phenotypic characteristics distinguish strains LMG 27748T, LMG 27749 and LMG 27882 from species of the genus Gluconobacter with validly published names and, therefore, we propose classifying them formally as representatives of a novel species, Gluconobacter cerevisiae sp. nov., with LMG 27748T ( = DSM 27644T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.059311-0