1887

Abstract

A halophilic, Gram-staining-positive, non-motile, endospore forming rod-shaped bacterial strain, S1LM8, was isolated from a sediment sample collected from an inland solar saltern located in La Malahá, Granada (Spain). Growth was observed in media containing 7.5–30 % total salts (optimum 15 % total salts), at pH 7–10 (optimum pH 8) and at 15–50 °C (optimum 35–38 °C). The predominant isoprenoid quinone was MK-7. It contained A1γ-type peptidoglycan with -diaminopimelic acid as the diagnostic diamino acid. The major cellular fatty acids were anteiso-C, iso-C, anteiso-C and iso-C. The G+C content of its genomic DNA was 38.2 mol%. The affiliation of strain S1LM8 with the species of the genus was determined by 16S rRNA gene sequence comparison. The most closely related species were YIM 012 with 99.8 % similarity, BH163 with 99.8 % similarity and ISL-17 with 98.1 % similarity between their 16S rRNA gene sequences. However, DNA–DNA relatedness between the novel isolate and the related species of the genus was less than 34 %. Based on the phylogenetic, phenotypic and chemotaxonomic features, a novel species, sp. nov. is proposed. The type strain is S1LM8 ( = CECT 8373 = DSM 27545).

Funding
This study was supported by the:
  • , Ministry of Science and Innovation (Spain) , (Award CGL2008-00447)
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2014-06-01
2020-07-13
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