1887

Abstract

The genus gen. nov. (to replace the illegitimate genus name ) is proposed for strains of Gram-negative bacteria causing corky root of lettuce, a widespread and important lettuce disease worldwide. Only one species of the genus was described, , which was subsequently reclassified as based on 16S rRNA gene sequences and the presence of sphingoglycolipid in the cell envelope. However, the genus is so diverse that further reclassification was deemed necessary. Twenty new gen. nov.- and -like isolates were obtained from lettuce or sow thistle roots, or from soil using lettuce seedlings as bait. These and previously reported isolates were characterized in a polyphasic study including 16S rRNA gene sequencing, DNA–DNA hybridization, DNA G+C content, whole-cell fatty acid composition, morphology, substrate oxidation, temperature and pH sensitivity, and pathogenicity to lettuce. The isolates causing lettuce corky root belonged to the genera gen. nov., , and gen. nov. More specifically, we propose to reclassify as gen. nov., comb. nov. (type strain, CA1 = LMG 17323 = ATCC 49355), and also propose the novel species sp. nov., sp. nov. and gen. nov., sp. nov. with the type strains NL9 ( = LMG 12560 = ATCC 51296), WI4 ( = LMG 11032 = ATCC 51292) and SP1 ( = LMG 12581 = ATCC 51289), respectively. Several strains isolated from lettuce roots belonged to the genus , but none of them were pathogenic.

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2014-04-01
2019-10-17
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