1887

Abstract

A facultatively anaerobic, Gram-reaction-positive, catalase- and oxidase-negative, rod-shaped bacterium isolated from an infected human wound caused by a dog bite was characterized by phenotypic and molecular genetic methods. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IMMIB Q2029717 was a member of the order of the class , displaying 91.6 % to 96 % sequence similarity with members of the family . Phylogentic trees generated by different algorithms indicated that the strain forms an independent phylogenetic line of descent that consistently clustered proximal to the base of the genus . Chemical studies revealed the presence of a cell-wall murein based on -lysine (type B1α), major menaquinone (MK-10) and a DNA G+C content of 56.9 mol%. The distinct phylogenetic position, ribotyping and matrix-assisted laser desorption/ionization time-of-flight MS profiles and the significant phenotypic differences clearly separate strain IMMIB Q2029717 from its nearest phylogenetic neighbour and support its classification as a representative of a novel genus and species, with the suggested name gen. nov., sp. nov. The type strain is IMMIB Q2029717 ( = DSM 27064 = CCUG 64069).

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2014-05-01
2019-10-22
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References

  1. Abrahamian F. M. , Goldstein E. J. C. . ( 2011; ). Microbiology of animal bite wound infections. . Clin Microbiol Rev 24:, 231–246. [CrossRef] [PubMed]
    [Google Scholar]
  2. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  3. Bauer A. W. , Kirby W. M. M. , Sherris J. C. , Turck M. . ( 1966; ). Antibiotic susceptibility testing by a standardized single disk method. . Am J Clin Pathol 45:, 493–496.[PubMed]
    [Google Scholar]
  4. Bruce J. . ( 1996; ). Automated system rapidly identifies and characterizes microorganisms in food. . Food Technol 50:, 77–81.
    [Google Scholar]
  5. Chhour K.-L. , Hinds L. A. , Jacques N. A. , Deane E. M. . ( 2010; ). An observational study of the microbiome of the maternal pouch and saliva of the tammar wallaby, Macropus eugenii, and of the gastrointestinal tract of the pouch young. . Microbiology 156:, 798–808. [CrossRef] [PubMed]
    [Google Scholar]
  6. Dewhirst F. E. , Klein E. A. , Thompson E. C. , Blanton J. M. , Chen T. , Milella L. , Buckley C. M. F. , Davis I. J. , Bennett M.-L. , Marshall-Jones Z. V. . ( 2012; ). The canine oral microbiome. . PLoS ONE 7:, e36067. [CrossRef] [PubMed]
    [Google Scholar]
  7. Evtushenko L. I. . ( 2012; ). Family XI. Microbacteriaceae . . In Bergey’s Manual of Systematic Bacteriology, vol. 5, part A, pp. 807–994. Edited by Goodfellow M. , Kämpfer P. , Busse H.-J. , Trujillo M. E. , Suzuki K. , Ludwig W. , Whitman W. B. . . New York:: Springer;.
    [Google Scholar]
  8. Evtushenko L. I. , Dorofeeva L. V. , Subbotin S. A. , Cole J. R. , Tiedje J. M. . ( 2000; ). Leifsonia poae gen. nov., sp. nov., isolated from nematode galls on Poa annua, and reclassification of ‘Corynebacterium aquaticum’ Leifson 1962 as Leifsonia aquatica (ex Leifson 1962) gen. nov., nom. rev., comb. nov. and Clavibacter xyli Davis et al. 1984 with two subspecies as Leifsonia xyli (Davis et al. 1984) gen. nov., comb. nov.. Int J Syst Evol Microbiol 50:, 371–380. [CrossRef] [PubMed]
    [Google Scholar]
  9. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  10. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  11. Gordon R. E. , Smith M. M. . ( 1955; ). Proposed group of characters for the separation of Streptomyces and Nocardia . . J Bacteriol 69:, 147–150.[PubMed]
    [Google Scholar]
  12. Kong H. H. , Oh J. , Deming C. , Conlan S. , Grice E. A. , Beatson M. A. , Nomicos E. , Polley E. C. , Komarow H. D. . & other authors ( 2012; ). Temporal shifts in the skin microbiome associated with disease flares and treatment in children with atopic dermatitis. . Genome Res 22:, 850–859. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lin Y. C. , Uemori K. , de Briel D. A. , Arunpairojana V. , Yokota A. . ( 2004; ). Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae . . Int J Syst Evol Microbiol 54:, 1669–1676. [CrossRef] [PubMed]
    [Google Scholar]
  14. Ludwig W. , Strunk O. , Westram R. , Richter L. , Meier H. , Yadhukumar , Buchner A. , Lai T. , Steppi S. . & other authors ( 2004; ). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef] [PubMed]
    [Google Scholar]
  15. Manaia C. M. , Nogales B. , Weiss N. , Nunes O. C. . ( 2004; ). Gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of ‘Brevibacterium helvolum’ DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov.. Int J Syst Evol Microbiol 54:, 783–789. [CrossRef] [PubMed]
    [Google Scholar]
  16. Mesbah M. , Premachandran U. , Whitman W. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  17. Rainey F. A. , Ward-Rainey N. , Kroppenstedt R. M. , Stackebrandt E. . ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:, 1088–1092. [CrossRef] [PubMed]
    [Google Scholar]
  18. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Schleifer K. H. . ( 1985; ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18:, 123–156. [CrossRef]
    [Google Scholar]
  20. Sturgeon A. , Stull J. W. , Costa M. C. , Weese J. S. . ( 2013; ). Metagenomic analysis of the canine oral cavity as revealed by high-throughput pyrosequencing of the 16S rRNA gene. . Vet Microbiol 162:, 891–898. [CrossRef] [PubMed]
    [Google Scholar]
  21. Takeuchi M. , Weiss N. , Schumann P. , Yokota A. . ( 1996; ). Leucobacter komagatae gen. nov., sp. nov., a new aerobic gram-positive, nonsporulating rod with 2,4-diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46:, 967–971. [CrossRef] [PubMed]
    [Google Scholar]
  22. Talan D. A. , Citron D. M. , Abrahamian F. M. , Moran G. J. , Goldstein E. J. C. . Emergency Medicine Animal Bite Infection Study Group ( 1999; ). Bacteriologic analysis of infected dog and cat bites. . N Engl J Med 340:, 85–92. [CrossRef] [PubMed]
    [Google Scholar]
  23. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  24. Yassin A. F. , Hupfer H. . ( 2006; ). Williamsia deligens sp. nov., isolated from human blood. . Int J Syst Evol Microbiol 56:, 193–197. [CrossRef] [PubMed]
    [Google Scholar]
  25. Yassin A. F. , Haggenel B. , Budzikiewicz H. , Schaal K. P. . ( 1993; ). Fatty acid and polar lipid composition of the genus Amycolatopsis: application of fast atom bombardment-mass spectrometry to structure analysis of underivatized phospholipids. . Int J Syst Bacteriol 43:, 414–420. [CrossRef]
    [Google Scholar]
  26. Yassin A. F. , Chen W. M. , Hupfer H. , Siering C. , Kroppenstedt R. M. , Arun A. B. , Lai W. A. , Shen F. T. , Rekha P. D. , Young C. C. . ( 2007; ). Lysobacter defluvii sp. nov., isolated from municipal solid waste. . Int J Syst Evol Microbiol 57:, 1131–1136. [CrossRef] [PubMed]
    [Google Scholar]
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