1887

Abstract

A Gram-stain-negative, non-spore-forming, rod-shaped bacterium, designated strain 1GB, was isolated from anodic biofilms of a glucose-fed microbial fuel cell. Strain 1GB was facultatively anaerobic and chemo-organotrophic, having both a respiratory and a fermentative type of metabolism, and utilized a wide variety of sugars as carbon and energy sources. Cells grown aerobically contained Q-8 as the major quinone, but excreted Q-9 and a small amount of Q-10 when cultured with an electrode serving as the sole electron acceptor. The G+C content of the genomic DNA of 1GB was 54.5 mol%. Multilocus sequence typing (MLST) analysis showed that strain 1GB represented a distinct lineage within the genus (98.5–99.4 % 16S rRNA gene sequence similarity and 94.0–96.5 % sequence similarity based on the three concatenated housekeeping genes , and . Strain 1GB exhibited DNA–DNA hybridization relatedness of 7–43 % with type strains of all established species of the genus . On the basis of these phenotypic, phylogenetic and genotypic data, the name sp. nov. is proposed for strain 1GB. The type strain is 1GB ( = NBRC 109676 = KCTC 32430).

Funding
This study was supported by the:
  • , Japanese Science and Technology Agency (JST
  • , Japanese Society for the Promotion of Science , (Award 23656324)
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2014-04-01
2020-07-12
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