1887

Abstract

A Gram-stain-positive, aerobic, endospore-forming, moderately halophilic rod, designated strain R1, was isolated from rice husks and subjected to a taxonomic study using a polyphasic approach. Strain R1 produced spherical or ellipsoidal endospores at a subterminal position in swollen sporangia, and was catalase- and oxidase-positive. The isolate grew optimally at 37 °C and pH 6.0–7.0, and could grow in the presence of up to 9 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R1 belongs to the genus . The closest relatives of strain R1 were subsp NCIB 3610, TF-12, and TF-11, with 16S rRNA gene sequence similarities of 96.0 %, 98.4 %, and 98.7 %, respectively. DNA–DNA relatedness values between the isolate and the reference strains were ≤42±3 %. The predominant menaquinones were MK-5 (50 %) and MK-7 (50 %). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylethanolamine. The major cellular fatty acids were iso-C (48.6 %) and anteiso-C (20.6 %), and the cell-wall diamino acid was -diaminopimelic acid. On the basis of 16S rRNA gene sequence analyses and chemotaxonomic and phenotypic characteristics, it is concluded that strain R1 represents a novel species of the genus , for which we propose the name sp. nov. The type strain is R1 ( = KACC 17217 = KCCM 90231 = JCM 19602).

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2014-08-01
2019-10-21
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