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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_4

sp. nov., isolated from chilled food Free

Abstract

A Gram-stain-negative, aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated J22, was isolated from fresh pasta filled with pork (tortellini) that had been stored at 6 °C. Based on 16S rRNA gene sequence analysis, strain J22 falls within the radiation of species of the genus . On the basis of 16S rRNA gene sequence similarities, the closest species phylogenetically are LMG 23400 (98.7 %), DSM 12126 (98.5 %) and DSM 2366 (98.3 %). The only isoprenoid quinone of strain J22 was menaquinone 7 (MK-7). The major fatty acids were iso-C (34.8 %), C 9 (24.9 %), C (7.3 %), C (4.8 %) and iso-C 3-OH (4.4 %). These chemotaxonomic characteristics supported the assignment of the isolate to the genus . DNA–DNA hybridization experiments showed that the DNA–DNA relatedness between strain J22 and its closest phylogenetic neighbour, LMG 23400, was less than 36 %. This indicated that the isolate was distinct from this species. Biochemical tests allowed strain J22 to be phenotypically differentiated from closely related species of the genus . As a consequence, strain J22 should be classified as a representative of a novel species of the genus for which the name sp. nov. is proposed. The type strain is J22 ( = DSM 27372 = CCUG 64422).

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2014-04-01
2024-04-24
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Pedobacter nutrimenti sp. nov., isolated from chilled food
Int J Syst Evol Microbiol 64, 1310 (2014); https://doi.org/10.1099/ijs.0.058677-0
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